In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection

Cho, Il Hoon; Irudayaraj, Joseph

doi:10.1016/j.ijfoodmicro.2013.02.025

Cited by 140 publications

(73 citation statements)

References 32 publications

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“…Traditional culturebased assays, which are regarded as the "gold standards" are widely, used by many organizations, especially by regulatory food safety agencies and food companies, because these are accepted in global regulation for trade and enable detection of low levels of pathogens [77]. However, standardized ways of detecting foodborne pathogenic bacteria by regulatory organizations (i.e., ISO, WHO, FDA) are often labor intensive and time consuming because of the sequence of the following processes: (i) pre-enrichment in non-selective broth (e.g., buffered peptone water, trypticase soy broth); (ii) selective bacterial enrichment using selective broth (e.g., Rappaport- This entire process usually requires 5 to 7 days to complete.…”

Section: Salmonella Detectionmentioning

confidence: 99%

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

Seockmo¹,

Eduardo²,

Thomas³

et al. 2016

J Nutr Food Sci

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Contaminated shell egg is one of the key Salmonella infection routes for human, causing food-borne illnesses. Multiple salmonellosis infections due to egg contamination still occur in developed countries even though preventive methods, such as vaccination or washing followed by rinsing process, are carried out following certified national standards. Recent outbreaks indicated that current strategies for Salmonella control need to be optimized to further minimize contamination of commercial eggs. Therefore, there is a critical need to develop more sensitive and rapid Salmonella detection methods. In this review, we address (i) egg production; (ii) preventive methods that minimize contamination; (iii) mechanisms of Salmonella contamination; (iv) Salmonella detection methods.

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Section: Salmonella Detectionmentioning

confidence: 99%

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

Seockmo¹,

Eduardo²,

Thomas³

et al. 2016

J Nutr Food Sci

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“…[8] CONCLUSIONS Immunological assays with different readouts have been extensively reported for the detection of Salmonella using magnetic particles. [27][28][29][30] However, in the literature there are no conclusive results about the influence of the size of the magnetic particles on the immunomagnetic separation and detection of Salmonella, as well as matrix effect studies. This paper addressed a comparison of the performance of both magnetic micro and nanoparticles in the immunomagnetic separation step for Salmonella using different media and samples by bacteria plating and microscopy studies.…”

Section: Preferred Position For Figmentioning

confidence: 99%

Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. A comparative study

Brandão

Liébana

Campoy

et al. 2015

Talanta

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“…Existing bacteria detection methods such as culturing (Allen et al 2004), immunology assays (ELISA) (Jain et al 2012;Cho and Irudayaraj 2013) and molecular biology methods (PCR) (Opet and Levin 2013; Liu et al 2013), are limited by low sensitivity, complex operation (Wang et al 2007) or high costs (Mollasalehi and Yazdanparast 2013). The requirement for big equipment is a major bottleneck for realtime and rapid detection of bacteria.…”

Section: Introductionmentioning

confidence: 99%

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

Wang

Liu

et al. 2017

Biomed Microdevices

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An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10 m, and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria. The microfluidic chip with interdigitated microelectrodes was manufactured by microfabrication methods. The interdigital microelectrode array has sufficient contact area with a bacterial suspension to improve enrichment efficiency and detection sensitivity. Bacteria in the interdigital microelectrode area of the microfluidic chip were firstly captured and enriched by pDEP. Then, in situ impedance detection of the enriched bacteria was realized by switching test conditions. Using the self-assembly microsystem, a novel quantitative detection method was established and demonstrated to detect Escherichia coli O157:H7. Experimental results showed that the detection limits of Escherichia coli O157:H7 was 5 × 10 cfu mL, and testing time was only 6 min under the optimized detection voltage of 100 mV and frequency of 500 KHz. The method was successfully used to detect Escherichia coli O157:H7 in synthetic chicken synthetic samples.

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In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection

Cited by 140 publications

References 32 publications

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. A comparative study

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

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