1996
DOI: 10.1002/(sici)1098-2825(1996)10:2<98::aid-jcla7>3.3.co;2-6
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In situ indirect fluorescent antibody: A new specific test to detect ongoing chagasic infections

Abstract: In situ immunofluorescence assay (ISIFA) was developed for the selection for treatment of chronically infected chagasic patients and their follow-up. Trypomastigotes flash fixed in situ on microscopic slides with 0.025% glutaraldehyde were used as antigen. ISIFA results were compared with complement-mediated lysis (CoML), membrane immunofluorescence (MbIFA), immunofluorescence assay (IFA) with epimastigotes, and xenodiagnosis (XENO). ISIFA was able to distinguish nontreated chagasic patients (geometric mean ti… Show more

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