In-situ polymerized polypyrrole nanoparticles immobilized poly(ε-caprolactone) electrospun conductive scaffolds for bone tissue engineering

Maharjan, Bikendra; Kaliannagounder, Vignesh Krishnamoorthi; Jang, Se Rim; Awasthi, Ganesh Prasad; Bhattarai, Deval Prasad; Choukrani, Ghizlane; Park, Chan Hee; Kim, Cheol Sang

doi:10.1016/j.msec.2020.111056

Cited by 83 publications

(63 citation statements)

References 69 publications

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“…Huang et al [32] investigated the behavior of silicone scaffolds with and without ES and found a significant increase in osteogenic protein expressions in the groups treated with ES compared with non-treated groups. Similar findings were also reported by Bikendra et al [33] using PCL/polypyrrole (PPy) scaffolds showing better MC3T3-E1cell adhesion, proliferation and differentiation when used together with ES.…”

Section: Discussionsupporting

confidence: 88%

In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering

et al. 2021

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Critical bone defects are considered one of the major clinical challenges in reconstructive bone surgery. The combination of 3D printed conductive scaffolds and exogenous electrical stimulation (ES) is a potential favorable approach for bone tissue repair. In this study, 3D conductive scaffolds made with biocompatible and biodegradable polycaprolactone (PCL) and multi-walled carbon nanotubes (MWCNTs) were produced using the extrusion-based additive manufacturing to treat large calvary bone defects in rats. Histology results show that the use of PCL/MWCNTs scaffolds and ES contributes to thicker and increased bone tissue formation within the bone defect. Angiogenesis and mineralization are also significantly promoted using high concentration of MWCNTs (3 wt%) and ES. Moreover, scaffolds favor the tartrate-resistant acid phosphatase (TRAP) positive cell formation, while the addition of MWCNTs seems to inhibit the osteoclastogenesis but present limited effects on the osteoclast functionalities (receptor activator of nuclear factor κβ ligand (RANKL) and osteoprotegerin (OPG) expressions). The use of ES promotes the osteoclastogenesis and RANKL expressions, showing a dominant effect in the bone remodeling process. These results indicate that the combination of 3D printed conductive PCL/MWCNTs scaffold and ES is a promising strategy to treat critical bone defects and provide a cue to establish an optimal protocol to use conductive scaffolds and ES for bone tissue engineering.

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Section: Discussionsupporting

confidence: 88%

In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering

et al. 2021

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“…Inherently, the chemical compositions (i.e., the phosphorus element and amino acid side groups) of the PAGP and PATGP had contributed to the promotion 25,46 . In the biomineralization study, the conductive PATGP‐based microspheres displayed stronger ability to induce mineral deposition than the nonconductive PAGP‐based microspheres, which were elucidated by charged surfaces of the conductive microspheres to capture more ions from SBF via electrostatic interactions 17,47,48 . This could be an favorable event to activate the osteogenic differentiation of BMSCs as well 22 .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, conductive, and osteocompatible polyorganophosphazene microscaffolds for the repair of infectious calvarial defect

Huang

Zheng

et al. 2021

J Biomedical Materials Res

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Many osteoconductive and osteoinductive scaffolds have been developed for promoting bone regeneration; however, failures would occur in osteogenesis when the defect area is significantly infected while the biomaterials have no antibacterial performances. Herein, a kind of multipurpose PATGP@PDA + Ag microspheres was prepared via emulsion method by using a conductive aniline tetramer (AT) substituted polyphosphazene (PATGP), followed by polydopamine (PDA) modification and silver nanoparticles (AgNPs) loading. The PATGP@PDA + Ag microspheres demonstrated a strong antibacterial activity against Staphylococcus aureus both in vitro and in vivo, while showing no cytotoxicity at an optimized AgNPs loading amount. Due to the electron‐donor structure of the AT moieties, the PATGP@PDA + Ag microspheres displayed antioxidant capacities to scavenge reactive oxygen species (ROS). Due to their phosphorus‐rich feature, the PATGP@PDA + Ag microspheres favored the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). As controls, nonconductive microspheres (PAGP@PDA, PAGP@PDA + Ag) were prepared similarly by using poly[(ethylalanine)(ethylglycyl)]phosphazene (PAGP). By co‐implanting these microspheres with S. aureus into rat calvarial defects, among them, it was determined that the PATGP@PDA + Ag microspheres achieved the most abundant neo‐bone formation, benefiting from their antibacterial, antioxidant and osteogenic activities. These results revealed that AgNPs loaded scaffolds made of conductive polyphosphazenes were promising for the regeneration of infected bone defects.

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“…For instance, electrospun polycaprolactone nanofibrous mats immobilized with polypyrrole nanoparticles via in-situ polymerization were prepared and used for culturing of MC3T3-E1 cells ( Fig. 17 ) [36] . The composite scaffold supported better adhesion, viability, proliferation and osteogenic differentiation of MC3T3-E1 cells, when compared with non-conductive polycaprolactone mats.…”

Section: Bone Regenerationmentioning

confidence: 99%

Electroconductive multi-functional polypyrrole composites for biomedical applications

Zare

Agarwal

Zarepour

et al. 2021

Applied Materials Today

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In-situ polymerized polypyrrole nanoparticles immobilized poly(ε-caprolactone) electrospun conductive scaffolds for bone tissue engineering

Cited by 83 publications

References 69 publications

In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering

In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering

Antibacterial, conductive, and osteocompatible polyorganophosphazene microscaffolds for the repair of infectious calvarial defect

Electroconductive multi-functional polypyrrole composites for biomedical applications

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