In-situ porcine corneal matrix hydrogel as ocular surface bandage

Yazdanpanah, Ghasem; Shah, Ritu; Somala, Sri Raghurama R; Anwar, Khandaker N.; Shen, Xiang; An, Song A.; Omidi, Meisam; Rosenblatt, Mark I.; Shokuhfar, Tolou; Djalilian, Ali R.

doi:10.1016/j.jtos.2021.04.004

Cited by 27 publications

(23 citation statements)

References 51 publications

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“…The hydrogel derived from decellularized cornea possesses unique advantages in preserving corneal stromal keratocyte cells, facilitating rapid re-epithelialization, and promoting corneal tissue regeneration, which have brought great interests in potential treatments of corneal diseases, especially for corneal wound healing [ 24 , 44 , 54 , 55 ]. However, the dECM hydrogel often suffer from their low mechanical strength and fast degradation, even after chemical crosslinking [ 56 ], which is unsatisfied for long-term repair of corneal defects with critical optical functional (i.e., enough transparency) and tissue regeneration requirements.…”

Section: Discussionmentioning

confidence: 99%

Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect

Shen

Zhao

et al. 2023

Bioactive Materials

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Section: Discussionmentioning

confidence: 99%

Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect

Shen

Zhao

et al. 2023

Bioactive Materials

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“… 105 Recently, Yazdanpanah et al reported the potential regenerative effects of an ocular bandage hydrogel made from decellularized porcine corneal ECM on a murine corneal epithelial wound healing model. 106 Using soft lithography, silk fibroin can be prepared as highly translucent films and altered to create nanoscale models to imitate the ECM structures. 107 These biomaterials are potential platforms for transplantable tissue-engineered corneal epithelial cell sheets or stem cell niche and may offer promising options for patients with limbal stem cell deficiency.…”

Section: Bioengineered Extracellular Matrixmentioning

confidence: 99%

Current and Emerging Therapies for Limbal Stem Cell Deficiency

Elhusseiny

Soleimani

Eleiwa

et al. 2022

Stem Cells Translational Medicine

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The corneal epithelium serves to protect the underlying cornea from the external environment and is essential for corneal transparency and optimal visual function. Regeneration of this epithelium is dependent on a population of stem cells residing in the basal layer of the limbus, the junction between the cornea and the sclera. The limbus provides the limbal epithelial stem cells (LESCs) with an optimal microenvironment, the limbal niche, which strictly regulates their proliferation and differentiation. Disturbances to the LESCs and/or their niche can lead to the pathologic condition known as limbal stem cell deficiency (LSCD) whereby the corneal epithelium is not generated effectively. This has deleterious effects on the corneal and visual function, due to impaired healing and secondary corneal opacification. In this concise review, we summarize the characteristics of LESCs and their niche, and present the current and future perspectives in the management of LSCD with an emphasis on restoring the function of the limbal niche.

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“…Another promising new approach for ECM scaffolds, particularly for stroma regeneration, is the use of decellularized porcine or human corneas [ 5 , 76 ]. This approach utilizes efficient decellularization of the corneas to remove cells and immunogenic antigens while preserving the structure and functionality of the ECM.…”

Section: Therapeutic Regeneration Of the Limbal Nichementioning

confidence: 99%

“…Another approach utilizing decellularization is the fabrication of bio-active hydrogels. These hydrogels are derived from digested decellularized corneas [ 76 , 82 ]. In the studies by our group, we have fabricated a thermoresponsive hydrogel from a decellularized porcine cornea extracellular matrix (COMatrix, Cornea Matrix) via digestion with pepsin/HCl [ 82 ].…”

Section: Therapeutic Regeneration Of the Limbal Nichementioning

confidence: 99%

“…This bio-active hydrogel is compatible with both epithelial and stromal cells, resulting in promising cell delivery vehicles for three-dimensional structures [ 42 ]. Moreover, in vitro studies have shown a proliferative effect of COMatrix on human corneal epithelial cells as well as enhancing corneal epithelial wound healing in in vivo animal models [ 76 ].…”

Section: Therapeutic Regeneration Of the Limbal Nichementioning

confidence: 99%

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The Limbal Niche and Regenerative Strategies

Amin

Jalilian

Katz

et al. 2021

Vision

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The protective function and transparency provided by the corneal epithelium are dependent on and maintained by the regenerative capacity of limbal epithelial stem cells (LESCs). These LESCs are supported by the limbal niche, a specialized microenvironment consisting of cellular and non-cellular components. Disruption of the limbal niche, primarily from injuries or inflammatory processes, can negatively impact the regenerative ability of LESCs. Limbal stem cell deficiency (LSCD) directly hampers the regenerative ability of the corneal epithelium and allows the conjunctival epithelium to invade the cornea, which results in severe visual impairment. Treatment involves restoring the LESC population and functionality; however, few clinically practiced therapies currently exist. This review outlines the current understanding of the limbal niche, its pathology and the emerging approaches targeted at restoring the limbal niche. Most emerging approaches are in developmental phases but show promise for treating LSCD and accelerating corneal regeneration. Specifically, we examine cell-based therapies, bio-active extracellular matrices and soluble factor therapies in considerable depth.

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In-situ porcine corneal matrix hydrogel as ocular surface bandage

Cited by 27 publications

References 51 publications

Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect

Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect

Current and Emerging Therapies for Limbal Stem Cell Deficiency

The Limbal Niche and Regenerative Strategies

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