In situ process monitoring and automated multi-parameter evaluation using optical coherence tomography during extrusion-based bioprinting

Yang, Shanshan; Chen, Qi; Xu, Mingzhen

doi:10.1016/j.addma.2021.102251

Cited by 16 publications

(19 citation statements)

References 29 publications

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“…Between 2016 and 2021 the number of publications on the topic automation in bioprinting and tissue engineering indicated a strong increase in interest 1 . Individual publications show groundbreaking results on how the use of automation solutions can help to monitor and adjust crucial process parameters and to draw conclusions about cell biological characteristics or allow the printing of complex 3D structures with hydrogels ( Hinton et al, 2015 ; Shi et al, 2018 ; Armstrong et al, 2019 ; Jin et al, 2021 ; Poologasundarampillai et al, 2021 ; Yang et al, 2021 ). At the same time, the number of patents and companies embracing 3D-Bioprinting continue to grow ( Santoni et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Scalable Biofabrication: A Perspective on the Current State and Future Potentials of Process Automation in 3D-Bioprinting Applications

Lindner

Blaeser

2022

Front. Bioeng. Biotechnol.

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Biofabrication, specifically 3D-Bioprinting, has the potential to disruptively impact a wide range of future technological developments to improve human well-being. Organs-on-Chips could enable animal-free and individualized drug development, printed organs may help to overcome non-treatable diseases as well as deficiencies in donor organs and cultured meat may solve a worldwide environmental threat in factory farming. A high degree of manual labor in the laboratory in combination with little trained personnel leads to high costs and is along with strict regulations currently often a hindrance to the commercialization of technologies that have already been well researched. This paper therefore illustrates current developments in process automation in 3D-Bioprinting and provides a perspective on how the use of proven and new automation solutions can help to overcome regulatory and technological hurdles to achieve an economically scalable production.

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Section: Discussionmentioning

confidence: 99%

Scalable Biofabrication: A Perspective on the Current State and Future Potentials of Process Automation in 3D-Bioprinting Applications

Lindner

Blaeser

2022

Front. Bioeng. Biotechnol.

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“…Compared with FF-OCT, OCT has a larger imaging FOV and imaging depth and has been widely used in materials science, in situ process monitoring, and tissue engineering. 80 – 83 , 93 – 95 But FF-OCT has isotropic micrometric resolution and has the ability of producing a histology-like image from a thick sample within a few milliseconds without physical slicing. Therefore, this technology is also known as optical biopsy.…”

Section: Applications Of Ff-octmentioning

confidence: 99%

Methods and applications of full-filed optical coherence tomography: a review

2022

J. Biomed. Opt.

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“…In the previous work, 3D extrusion-based bioprinter-associated optical coherence tomography (3D P-OCT) has been proposed[ 19 ]. OCT is a non-destructive, label-free, high-resolution, and fast tomographic imaging technique that are widely used in the biomedical and industrial testing fields[ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…OCT enables 3D volumetric imaging with micron-scale resolution over centimeter length scales and 3D P-OCT enables large-field full-depth imaging to meet the imaging requirements of large structures. 3D P-OCT can provide in situ process monitoring and multi-parameter evaluation layer by layer during extrusion-based bioprinting including LT, FS, layer fidelity, and 3D structure quantitative analysis, including material volume, VP, and PC[ 19 ]. This study mainly focuses on in situ defect detection and timely feedback control for print parameter compensation and defect repair.…”

Section: Introductionmentioning

confidence: 99%

In situ defect detection and feedback control with three-dimensional extrusion-based bioprinter-associated optical coherence tomography

Yang¹,

Chen²,

Xu³

2022

IJB

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Extrusion-based three-dimensional (3D) bioprinting is one of the most common methods used for tissue fabrication and is the most widely used additive manufacturing technique in all industries. In extrusion-based bioprinting, printing defects related to material deposition errors lead to a significant deviation from shape to function between the printed construct and design model. Using 3D extrusion-based bioprinter-associated optical coherence tomography (3D P-OCT), an in situ defect detection and feedback system was presented based on the accurate defect analysis and location, and a pre-built feedback mechanism. Using 3D P-OCT, multi-parameter quantification of the material deposition was carried out in real time, including the filament size, layer thickness, and layer fidelity. The material deposition errors under different paths were quantified and located specifically, including the start-stop points, straight-line path, and turnarounds. The pre-built feedback mechanism involving the control inputs, such as printing path, pressure, and velocity, provided the basis for in situ defect detection and real-time feedback control. In particular, the second printing repair can be performed after the broken filament defect is detected and located. After printing, fidelity can be quantitatively analyzed based on the point cloud registration between the 3D P-OCT result and the design model. In conclusion, 3D P-OCT enables in situ defect detection and feedback control, broken filament repair, and 3D fidelity analysis to achieve high-fidelity printing from shape to function.

show abstract

In situ process monitoring and automated multi-parameter evaluation using optical coherence tomography during extrusion-based bioprinting

Cited by 16 publications

References 29 publications

Scalable Biofabrication: A Perspective on the Current State and Future Potentials of Process Automation in 3D-Bioprinting Applications

Scalable Biofabrication: A Perspective on the Current State and Future Potentials of Process Automation in 3D-Bioprinting Applications

Methods and applications of full-filed optical coherence tomography: a review

In situ defect detection and feedback control with three-dimensional extrusion-based bioprinter-associated optical coherence tomography

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