In situ self-assembly of peptides in glucan particles for macrophage-targeted oral delivery

Zhang, Xu; Zhao, Yuanyuan; Xu, Yi; Pan, Yuanming; Chen, Fei; Kumar, Anil; Zou, Guozhang; Liang, Xing‐Jie

doi:10.1039/c4tb00626g

Cited by 19 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, ABZ-GPs can be used as a targeting vector for macrophages. From 1 to 2 h, the uptake efficiency of the ABZ-GPs rapidly increased; however, the fluorescence intensity at 4 h was significantly reduced or even lower than that at 1 h. Our findings differ to those of a previous study that reported a maximum uptake of GP capsules by macrophages at 8 h ( 41 ). The reason for this difference may be related to the preparation process of the ABZ-GPs and the characteristics of the drug itself.…”

Section: Resultscontrasting

confidence: 99%

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

Liu¹,

Yang²,

Zhu³

et al. 2022

Ann Transl Med

View full text Add to dashboard Cite

Background: Glucan particles (GPs) are derived from the Saccharomyces cerevisiae cell wall. The hollow particles composed of β-1,3-D-glucan have been extensively studied in terms of immune regulation and macrophage-targeted drug delivery. Albendazole (ABZ) is a benzimidazole drug with good anti-parasitic activity and is the drug recommended by the World Health Organization for the first-line treatment of hydatid disease.Methods: A dynamic light scatterometer, scanning electron microscope, and transmission electron microscope were used to characterize the ABZ-GPs. High-Performance Liquid Chromatography (HPLC), Laser Scanning Confocal Microscope (LSCM), and an in vivo small animal imaging system were used to evaluate theability of ABZ-GPs to be recognized by macrophages, whether ABZ-GPs are more readily absorbed and eliminated in the blood than the original ABZ drug in rats, and the ability of ABZ-GPs to target the mouse liver. Results:The ABZ-GPs were successfully constructed to achieve fluorescence, magnetic resonance imagining, and laser confocal microscopy imaging. The glucan shell effectively protects ABZ from enzymatic degradation and from being pumped out in the gastrointestinal tract. The analysis of ABZ and its major metabolite albendazole sulfoxide in the rat plasma and mouse liver showed that compared to the ABZ suspension group, the degradation of ABZ-GPSs in the blood was low, and the targeting of ABZ-GPSs in the liver was significantly enhanced. Conclusions:In the oral treatment of hepatic hydatid disease, GPs can be used as carriers to achieve the targeted transport of ABZ, which in turn can be used for the targeted therapy of liver echinococcosis. Thus, ABZ-GPs may be a promising form of targeted therapy.

show abstract

Section: Resultscontrasting

confidence: 99%

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

Liu¹,

Yang²,

Zhu³

et al. 2022

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…These results suggest that phagocytosis of NYPs by macrophages is primarily attributed to the recognition of β-glucan by dectin-1, which is in line with previous reports. 10,20 As well, it is clear that laminarin is a competitive inhibitor of dectin-1 for βglucan, as the stronger inhibiting effect was obtained by increasing the amount of added laminarin. Similarly, when the concentration of NYP suspension was set to 50 μg/mL, the uptake inhibition was weakened accordingly at 0.1 or 1 mg/mL of laminarin, as shown in Figures S2−3.…”

Section: Molecular Pharmaceuticsmentioning

confidence: 93%

“…The cellular uptake of double fluorescence-labeled NYPs was performed on Raw 264.7 cells. ,, RBITC was selected to label the YP shells, and a hydrophobic probe coumarin-6 was loaded into the core of particles. The 12-well sterile plate was used to culture the cells with a concentration of 1 × 10 5 cells/mL for 24 h. Raw 264.7 cells were first incubated with NYP suspensions at different concentrations of 1, 10, 20, and 50 μg/mL for 4 h. To confirm a time-dependent behavior of cellular uptake of NYPs, the cells were incubated with NYPs (20 μg/mL) for 2, 4, and 8 h. At these time points, the cells were rinsed with PBS and fixed in 4% paraformaldehyde solution.…”

Section: Methodsmentioning

confidence: 99%

“…Laminarin is a soluble β-1,3-glucan which could also be recognized by the dectin-1 receptor expressed on macrophage cells. In this study, laminarin was used as an inhibitor to study the uptake mechanisms of NYPs. , The cells were seeded in a 12-well sterile plate and incubated with NYPs suspensions with or without added laminarin. The concentration of laminarin was set at 0.1 and 1 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, YPs are ideal to be applied as a vehicle for targeting phagocytic cells, especially intestinal M cells. Until now, YPs have been primarily employed to encapsulate and orally deliver soluble macromolecules, e.g., DNA, siRNA, , vaccines, − and therapeutic proteins. − However, the employment of YPs for oral delivery of active small molecules is quite limited by YPs, as most major small molecule drugs are small in size, neutral in charge, and insoluble in water, and they are thus rarely steadily entrapped in YPs. ,, To solve this problem, a nano-in-micro method, that is, entrapping drug-loaded nanocarriers in the YPs, can be applied in development of new oral delivery systems. Nanoparticles (NPs), with different physicochemical properties, can be selected to realize orally targeted delivery of small molecule drugs, such as CTX, by packaging into YPs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Entrapping of Nanoparticles in Yeast Cell Wall Microparticles for Macrophage-Targeted Oral Delivery of Cabazitaxel

et al. 2018

View full text Add to dashboard Cite

In this work, a nano-in-micro carrier was constructed by loading polymer-lipid hybrid nanoparticles (NPs) into porous and hollow yeast cell wall microparticles (YPs) for macrophage-targeted oral delivery of cabazitaxel (CTX). The YPs, primarily composed of natural β-1,3-d-glucan, can be recognized by the apical membrane receptor, dectin-1, which has a high expression on macrophages and intestinal M cells. By combining electrostatic force-driven self-deposition with solvent hydration/lyophilization methods, the positively charged NPs loaded with CTX or fluorescence probes were efficiently packaged into YPs, as verified by scanning electron microscope (SEM), atomic force mircoscope (AFM), and confocal laser scanning microscopy (CLSM) images. NP-loaded YPs (NYPs) showed a slower in vitro drug release and higher drug stability compared with NPs in a simulated gastrointestinal environment. Biodistribution experiments confirmed a widespread distribution and extended retention time of NYPs in the intestinal tract after oral administration. Importantly, a large amount of NYPs were primarily accumulated and transported in the intestinal Peyer's patches as visualized in distribution and absorption site studies, implying that NYPs were mainly absorbed through the lymphatic pathway. In vitro cell evaluation further demonstrated that NYPs were rapidly and efficiently taken up by macrophages via receptor dectin-1-mediated endocytosis using a mouse macrophage RAW 264.7 cell line. As expected, in the study of in vivo pharmacokinetics, the oral bioavailability of CTX was improved to 32.1% when loaded in NYPs, which is approximately 5.7 times higher than that of the CTX solution, indicating the NYPs are efficient for oral targeted delivery. Hence, this nano-in-micro carrier is believed to become a hopeful alternative strategy for increasing the oral absorption of small molecule drugs.

show abstract

Self‐assembly of Peptides and Chromophores for Design of Theranostic Nanodrugs and Cancer Precision Therapy

Chang,

Xing,

Yan

2024

Peptide Self‐Assembly and Engineering

View full text Add to dashboard Cite

In situ self-assembly of peptides in glucan particles for macrophage-targeted oral delivery

Cited by 19 publications

References 24 publications

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

Entrapping of Nanoparticles in Yeast Cell Wall Microparticles for Macrophage-Targeted Oral Delivery of Cabazitaxel

Self‐assembly of Peptides and Chromophores for Design of Theranostic Nanodrugs and Cancer Precision Therapy

Contact Info

Product

Resources

About