In situ studies of pellicle formation on hydroxyapatite discs

Smith, A. M.; Bowen, Willis H.

doi:10.1016/s0003-9969(99)00141-7

Cited by 90 publications

(77 citation statements)

References 41 publications

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“…Therefore, these authors hypothesise a two-stage model of pellicle formation. Vacca-Smith and Bowen [47] recorded that the amounts of amylase and lysozyme detected on hydroxyapatite discs after in vivo exposure for different times remained stable already after 5 min of exposure in the mouth. Thus, we may consider a period of 30 min sufficient for the formation of a layer of salivary proteins on specimens of denture base material, at the same time minimising the risk of formation of a microbial plaque.…”

Section: Discussionmentioning

confidence: 99%

“…Thus it is conceivable that different desorbing solutions may yield different results on the desorbed salivary components from the pellicle layer. Vacca-Smith and Bowen [47] desorbed pellicle proteins from hydroxyapatite discs by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer. Carlén et al [6,7] used rubbing with sponges combined with desorption by A salivary pellicle forms very quickly onto the tooth surface.…”

Section: Discussionmentioning

confidence: 99%

“…The adsorption of salivary proteins is a selective process [20,33,47]. Whereas covalent binding of salivary components after adsorption is discussed for mucosal surfaces [3,4], the formation of a layer of salivary proteins on teeth as well as on dental materials is based on noncovalent binding.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative determination of salivary components in the pellicle on PMMA denture base material

Göcke¹,

Gerath

Schwanewede

2002

Clinical Oral Investigations

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The formation of a salivary pellicle is a protective mechanism of the body for all surfaces in the oral cavity. The nature of the substrate may influence the composition of the pellicle. The aim of this study was to investigate the quantitative composition and individual variation of the salivary pellicle formed on denture base material (PMMA). Cylindrical specimens of PMMA were carried in the mouth and then desorbed with a 0.5-M sodium chloride solution. The solution was analysed for total protein, alpha-amylase, total proteases, protease inhibitors, secretory immunoglobulin A, immunoglobulin G, peroxidases, thiocyanate, lysozyme, and calcium content. All investigated salivary components could be found unequivocally in the desorption solution, indicating that a salivary pellicle had formed on the surface of the PMMA. Large coefficients of variation indicate large individual variations in the adsorbed amounts. The data also point to large intraindividual variations for the bound salivary components. Only the protease inhibitors revealed a strong positive correlation of the bound activity to the salivary activity. It is hypothesised that differences in the bound amounts of antimicrobial components might influence the microbial colonisation of denture bases and that protease inhibitors could be meaningful for the spread of the yeast Candida albicans from denture base material to the oral mucosa and thus might be an explanation for different susceptibility to denture base stomatitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Quantitative determination of salivary components in the pellicle on PMMA denture base material

Göcke¹,

Gerath

Schwanewede

2002

Clinical Oral Investigations

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“…In vitro studies provided strong evidence that acidic proline rich proteins (PRPs), statherin and histatins bind preferentially to hydroxyapatite (HA) and are the major contributors to the acquired enamel pellicle (Lendenmann et al, 2000;Van Nieuw Amerongen et al, 2004). Indeed, this group of peptides contains phosphoserines as posttranslational modifications, which are thought to be a major contributing factor to their high affinity to HA (Bennick et al, 1983;Lamkin et al, 1991;Vacca-Smith and Bowen, 2000). In addition, they have been described as being related to remineralization phenomena occurring at the tooth surface, which is kept in a supersaturated calcium state (Lendenmann et al, 2000;Van Nieuw Amerongen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The role of salivary peptides in dental caries

Vitorino

Lobo²,

Duarte

et al. 2005

Biomed. Chromatogr.

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Dental caries is a complex disease, characterized by demineralization of tooth structure. With a protective role, several salivary phosphopeptides appear to be involved in remineralization processes, delaying the loss of tooth structure. In this work we have correlated peptide saliva composition with dental caries susceptibility through the analysis of saliva and hydroxyapatite-adsorbed salivary peptides samples. Saliva samples were obtained from two groups, a caries-free and a cariessusceptible group, and were analysed using HPLC-MS and a sequential extraction with 6 m of guanidine followed by tri fluoroacetate. Data analysis has allowed us to verify a strong correlation between large amounts phosphopeptides (PRP1/3, histatin 1 and statherin), and the absence of dental caries, which reinforces the importance of these peptides in the maintenance of tooth integrity. In addition, in the caries-susceptible group a high number of peptide fragments was observed, suggesting a high proteolytic activity.

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“…While the chemical analysis of the molecules adsorbed on the enamel surface provides information of the composition of the pellicle layer, especially with regard to its main protein constituents (15,18,19), no conclusive information about its morphology has been obtained with this method. This information may be obtained by SEM and TEM (10,20).…”

Section: Introductionmentioning

confidence: 99%