In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

Combes, Jeanne; Imatoukene, Nabila; Moussa, Marwen; Coquart, Nicolas; Chemarin, Florian; Athès, Violaine; Fojcik, Clémentine; Chadni, Morad; Ioannou, Irina; Lopez, Michel; Allais, Florent

doi:10.1016/j.seppur.2022.121083

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…Furthermore, it strengthens the perspective that the citrus PW effluent is a high‐quality enzyme inducer. Combes et al 37 . noted that, during LLE using organic solvents, pH was a factor that considerably affected the extraction of the target metabolite ( trans ‐ p ‐coumaric acid), where a yield of 89% was observed at pH 4.5 that decreased considerably to 45% at pH 6.0.…”

Section: Resultsmentioning

confidence: 99%

“…36 Furthermore, it strengthens the perspective that the citrus PW effluent is a high-quality enzyme inducer. Combes et al 37 noted that, during LLE using organic solvents, pH was a factor that considerably affected the extraction of the target metabolite (trans-p-coumaric acid), where a yield of 89% was observed at pH 4.5 that decreased considerably to 45% at pH 6.0. The effect of pH can also be seen in the recovery of the Lac enzyme in the bottom phase and in the recovery of contaminating proteins in the top phase, as shown in Table 2.…”

Section: Effect Of Ph On Partitioningmentioning

confidence: 99%

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Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Santana,

Barros,

Canuto

et al. 2023

J Sci Food Agric

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BackgroundThis study explores the use of liquid‐liquid extraction (LLE) with thermosensitive polymers for producing laccase (Lac) from Pleurotus sajor‐caju. This process leverages liquid waste from the citrus industry, specifically pulp wash. The research delves into extractive fermentation and thermoseparation, both processes were facilitated by a polymer exhibiting a lower critical solution temperature (LCST) transition.ResultsKey factors considered include the choice of polymer, its concentration, pH, separation temperature, and the behavior of the polymer‐rich phase post‐extractive fermentation concerning LCST. Notably, under conditions of 45% by weight of Pluronic L‐61 and a pH of 5.0 at 25°C, the laccase was achieved an enhancement in the purification factor (PF) of 28.4‐fold, compared to the laccase obtained directly from the fermentation process on the 8th day. There was an 83.6% recovery of the laccase enzyme in the bottom phase of the system. Additionally, the unique properties of Pluronic L‐61, which can induce phase separation and also allow for thermoseparation, led to a secondary fraction (aqueous solution) of laccase with PF of 2.1 ± 0.1‐fold (at 32 ± 0.9 °C and 30 ± 0.3 min without stirring) from polymeric phase (top‐phase). The FTIR analysis validated the separation data, particularly highlighting the α‐helix content in the Amide I region (1600 ‐ 1700 cm‐1).ConclusionIn summary, the insights from this study pave the way for broader industrial applications of these techniques, underscoring benefits like streamlined process integration, heightened selectivity, and superior separation efficacy.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Ph On Partitioningmentioning

confidence: 99%

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Santana,

Barros,

Canuto

et al. 2023

J Sci Food Agric

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“…Another recent example is the fermentation of p -coumaric acid by engineered S. cerevisiae with an in-line hollow-fiber membrane unit. 89 This fermentation had two key challenges: p -coumaric acid has very low solubility in water, and can inhibit biocatalytic activity. The extraction unit, in which the aqueous broth flowed into the lumen side, was used mainly to recover p -coumaric acid from the fermentation broth (Fig.…”

Section: Liquid–liquid Extraction For Bioprocess Intensificationmentioning

confidence: 99%

Advances in in situ and in-line liquid–liquid extraction for bioprocess intensification

Chaichol

Weeranoppanant

2023

React. Chem. Eng.

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“…Interestingly, p-coumaric acid, a natural cinnamic acid present in numerous plants, and its esters derivatives exhibit antifungal properties [11,16]. While this compound can be extracted from numerous agricultural by-products [17,18], its bioproduction from engineered strains is also demonstrated and could unlock some purification drawbacks [19,20]. Moreover, it has already been established that alkyl chains with at least eight carbons can efficiently destabilize fungal cell membranes, leading to moderate antifungal activity.…”

Section: Introductionmentioning

confidence: 99%

Chemo-Enzymatic Synthesis and Biological Assessment of p-Coumarate Fatty Esters: New Antifungal Agents for Potential Plant Protection

Thaeder,

Stanek,

Couvreur

et al. 2023

Molecules

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One trend in agriculture is the replacement of classical pesticides with more ecofriendly solutions, such as elicitation, which is a promising approach consisting of stimulating the natural immune system of a plant to improve its resistance to pathogens. In this fashion, a library of p-coumaric-based compounds were synthesized in accordance with as many principles of green chemistry as possible. Then, these molecules were tested for (1) the direct inhibition of mycelium growth of two pathogens, Botrytis cinerea and Sclerotinia sclerotiorum, and (2) plasma membrane destabilization in Arabidopsis and rapeseed. Finally, the protective effect was evaluated on an Arabidopsis/B. cinerea pathosystem. Total inhibition of the growth of both fungi could be achieved, and significant ion leakage was observed using dihydroxylated fatty p-coumarate esters. A direct effect on plants was also recorded as a ca. three-fold reduction in the necrosis area.

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In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

Cited by 9 publications

References 46 publications

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

Advances in in situ and in-line liquid–liquid extraction for bioprocess intensification

Chemo-Enzymatic Synthesis and Biological Assessment of p-Coumarate Fatty Esters: New Antifungal Agents for Potential Plant Protection

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