In tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control

González-Pérez, Lien; Perrotta, Lara; Acosta, Alexis; Orellana, Esteban A.; Spadafora, Natasha D.; Bruno, Leonardo; Bitonti, Beatrice M.; Albani, Diego; Cabrera, Juan Carlos; Francis, Dennis; Rogers, Hilary J.

doi:10.1007/s11033-014-3566-y

Cited by 19 publications

(10 citation statements)

References 75 publications

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“…Xyloglucans are the most common hemicelluloses in dicot primary cell walls but they are scarce in monocots [64]. The primary function of xyloglucan is the formation of a network with cellulose microfibrils in the cell walls but some reports have also demonstrated its role in cell signalling [65] and as a storage material in seeds [63]. Our work demonstrated that the hemicellulose that is recognised by the LM25 antibodies (for xyloglucan) was present in the cell walls and the antibody that was used for its detection gave the most abundant signals among all of the antibodies that were used.…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon

et al. 2016

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Brachypodium distachyon L. Beauv. (Brachypodium) is a species that has become an excellent model system for gaining a better understanding of various areas of grass biology and improving plant breeding. Although there are some studies of an in vitro Brachypodium culture including somatic embryogenesis, detailed knowledge of the composition of the main cell wall components in the embryogenic callus in this species is missing. Therefore, using the immunocytochemical approach, we targeted 17 different antigens of which five were against the arabinogalactan proteins (AGP), three were against extensins, six recognised pectic epitopes and two recognised hemicelluloses. These studies were complemented by histological and scanning electron microscopy (SEM) analyses. We revealed that the characteristic cell wall components of Brachypodium embryogenic calli are AGP epitopes that are recognised by the JIM16 and LM2 antibodies, an extensin epitope that is recognised by the JIM11 antibody and a pectic epitopes that is recognised by the LM6 antibody. Furthermore, we demonstrated that AGPs and pectins are the components of the extracellular matrix network in Brachypodium embryogenic culture. Additionally, SEM analysis demonstrated the presence of an extracellular matrix on the surface of the calli cells. In conclusion, the chemical compositions of the cell walls and ECMSN of Brachypodium callus show spatial differences that correlate with the embryogenic character of the cells. Thus, the distribution of pectins, AGPs and hemicelluloses can be used as molecular markers of embryogenic cells. The presented data extends the knowledge about the chemical composition of the embryogenic callus cells of Brachypodium.

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Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon

et al. 2016

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“…A less well‐characterized wall fragment is xyloglucan oligosaccharide (XGO). Tobacco BY‐2 (cv Bright Yellow 2) cell cultures treated with XGO exhibit an increase in fresh weight and an up‐regulation of genes related to abiotic and biotic stresses (González‐Pérez et al ., ), but whether XGO acts as a signal in growth regulation and stress response warrants further investigation.…”

Section: Cell Wall Integrity Sensingmentioning

confidence: 99%

A wall with integrity: surveillance and maintenance of the plant cell wall under stress

2019

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Summary The structural and functional integrity of the cell wall needs to be constantly monitored and fine‐tuned to allow for growth while preventing mechanical failure. Many studies have advanced our understanding of the pathways that contribute to cell wall biosynthesis and how these pathways are regulated by external and internal cues. Recent evidence also supports a model in which certain aspects of the wall itself may act as growth‐regulating signals. Molecular components of the signaling pathways that sense and maintain cell wall integrity have begun to be revealed, including signals arising in the wall, sensors that detect changes at the cell surface, and downstream signal transduction modules. Abiotic and biotic stress conditions provide new contexts for the study of cell wall integrity, but the nature and consequences of wall disruptions due to various stressors require further investigation. A deeper understanding of cell wall signaling will provide insights into the growth regulatory mechanisms that allow plants to survive in changing environments.

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“…Previous studies indicated that Xh may play a role in the regulation of plant growth and development in different plant species ( Fry et al, 1993 ; Vargas-Rechia et al, 1998 ). Indeed, treatment with Xh of tobacco cell cultures and whole plants results in accelerated cell elongation and division, changes in primary root growth, and upregulation of defense-related gene expression ( González-Pérez et al, 2014 ). Moreover, Xh can increase plant resistance to abiotic stress, in particular cold or water stress, when used at low concentrations ( Salvador and Lasserre, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana

et al. 2018

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Damage-associated molecular patterns (DAMPs) are endogenous molecules that can activate the plant innate immunity. DAMPs can derive from the plant cell wall, which is composed of a complex mixture of cellulose, hemicellulose, and pectin polysaccharides. Fragments of pectin, called oligogalacturonides (OG), can be released after wounding or by pathogen-encoded cell wall degrading enzymes (CWDEs) such as polygalacturonases (PGs). OG are known to induce innate immune responses, including the activation of mitogen-activated protein kinases (MAPKs), production of H2O2, defense gene activation, and callose deposition. Thus, we hypothesized that xyloglucans (Xh), derived from the plant cell wall hemicellulose, could also act as an endogenous elicitor and trigger a signaling cascade similar to OG. Our results indicate that purified Xh elicit MAPK activation and immune gene expression in grapevine (Vitis vinifera) and Arabidopsis (Arabidopsis thaliana) to trigger induced resistance against necrotrophic (Botrytis cinerea) or biotrophic (Hyaloperonospora arabidopsidis) pathogens. Xh also induce resveratrol production in grapevine cell suspension and callose deposition in Arabidopsis which depends on the callose synthase PMR4. In addition, we characterized some signaling components of Xh-induced immunity using Arabidopsis mutants. Our data suggest that Xh-induced resistance against B. cinerea is dependent on the phytoalexin, salicylate, jasmonate, and ethylene pathways.

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In tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control

Abstract: In tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control.--Manuscript Draft--

Cited by 19 publications

References 75 publications

Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon

Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon

A wall with integrity: surveillance and maintenance of the plant cell wall under stress

The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana

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