In Utero Gene Delivery Using Chitosan-DNA Nanoparticles in Mice

Yang, Patrick T.; Hoang, Lien; Jia, William; Skarsgard, Erik D.

doi:10.1016/j.jss.2010.05.039

Cited by 20 publications

(4 citation statements)

References 44 publications

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“…Recent advances in the use of nanocarriers for gene transfer into reproductive tissues, gametes, and embryos are rapidly creating a powerful tool with which to manipulate and study pathophysiological mechanisms in the reproductive sciences. [25][26][27] For example, our group has recently demonstrated the ability of MSNPs to bind with mammalian sperm without negative effects upon key semen parameters. 22 In addition, customization of the aforementioned nanoparticles with a cell-penetrating peptide coating has been shown to increase the rates of association with sperm.…”

Section: Discussionmentioning

confidence: 99%

Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research

et al. 2016

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Coward, "Development of a laseractivated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research," J. Biomed. Opt. 21(11), 115002 (2016), doi: 10.1117/1.JBO.21.11.115002. Abstract. Nanoparticles have revolutionized medical research over the last decade. One notable emerging area of nanomedicine is research developments in the reproductive sciences. Since increasing evidence indicates links between abnormal gene expression and previously unexplained states of infertility, there is a strong impetus to develop tools, such as nanoparticle platforms, to elucidate the pathophysiological mechanisms underlying such states. Mesoporous silica nanoparticles (MSNPs) represent a powerful and safe delivery tool for molecular and genetic investigations. Nevertheless, ongoing progress is restricted by low efficiency and unpredictable control of cargo delivery. Here, we describe for the first time, the development of a laser-activated MSNP system with heat-responsive cargo. Data derived from human embryonic kidney cells (HEK293T) indicate that when driven by a heat-shock promoter, MSNP cargo exhibits a significantly increased expression following infrared laser stimulus to stimulate a heat-shock response, without adverse cytotoxic effects. This delivery platform, with increased efficiency and the ability to impart spatial and temporal control, is highly useful for molecular and genetic investigations. We envision that this straightforward stimuli-responsive system could play a significant role in developing efficient nanodevices for research applications, for example in reproductive medicine. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Discussionmentioning

confidence: 99%

Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research

et al. 2016

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“…There are ongoing studies concerning optimization of in utero treatment of embryo renal anomalies by gene therapy. Nanomaterial-mediated gene transfer into foetal tissues was demonstrated by Yang et al (2011). The authors utilized intra-amniotic injections into mouse embryos of chitosan NPs conjugated with the enhanced green fluorescent protein (EGFP) gene and observed expression of the transgene in the alveolar epithelium of the lungs and the luminal intestinal epithelium.…”

Section: Embryogenesis During Embryogenesis Thementioning

confidence: 99%

Nanobiotechnology in reproduction – pros and cons. A review

Zielińska‐Górska

Górski

2015

J. Anim. Feed Sci.

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“…Chitosan nanoparticles were also synthesized by coacervation method for high association of DNA with intestinal Caco-2 cells and these nanoparticles protected the plasmid against nuclease degradation [22]. In 2010, Yang et al demonstrated that chitosan-EGFP (epidermal growth factor protein) nanoparticles formed stable aggregates in murine amniotic fluid (AF) and achieved short-term transgene expression in pup lung and intestine [23]. Several studies have reported the feasibility of direct gene transfer by using chitosan-nanoparticles into fish through the oral delivery to develop immunological protection against various diseases [24][25][26][27].…”

Section: Chitosanmentioning

confidence: 99%