In vitro Acetyl Cholinesterase Inhibitory assay of Acacia catechu Willd Ethanolic Seed Extract

Abstract

Aim & objective:The aim of this study was to evaluate acetyl cholinesterase inhibitory activity of Acacia catechu ethanolic seed extract to introduce a new source for management of Alzheimer's disease. Background: Alzheimer's disease is a complex, multifactorial, progressive, neurodegenerative disease primarily affecting the elderly population and is estimated to account for 50-60% of dementia cases in persons over 65 years of age. It is likely that the inhibition of acetyl cholinesterase by Acacia catechu ethanolic seed extract that is rich in flavonoids and antioxidants may aid in the protection of neurodegenerative disorders and ultimately Alzheimer's disease. Methods: Anti cholinesterase activity is determined by adopting In vitro standard protocol. Results: According to the obtained results, the inhibitory activity (IC 50 values, μg /ml) of extracts was 204.38 ± 2.54 µg/ ml. Conclusion: The results indicated and confirmed the traditional use of Acacia catechu ethanolic seed extract for management of central nervous system disorders. It showed the moderate activity in inhibition of acetyl cholinesterase at various concentrations. However, further investigations on identification of active components in the extracts are needed.

Searching for New Natural Inhibitors of Acetylcholinesterase Enzyme

“…Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE,pseudocholinesterase) enzymes were inhibited by methanolic extracts of grape (Vitisvinifera L.) seeds [30]. In studies with Acacia catechu willd, Cola acuminata, Cuminum cyminum and Lawsonıa Inermıs (Henna) seeds, IC50 values of seed extracts were found as 204.38 = ± 2.54 (ug/mL) [31], 14.6 ± 1.04 (𝜇g/mL) [32], 0.437μg/mL [33] and 66.6 (mg/L) [34] respectively. Similar to the literature, it was observed that selected seed extracts had inhibitory effects on the enzyme activity (Table 1, Figure 2).…”

Review on the Restorative Impact of Acacia Arabica

“…Though, there is only one clinical trial that examined the effectiveness of this medicinal supplement. Flavocoxid was found to decrease the physical symptoms linked with knee osteoarthritis in a double-blind randomized prohibited examination using 253 volunteers diagnosed with low-to-me osteoarthritis 72 . Naproxen (440 mg/day) was used and the clinical results of the botanical mixture were similar with or even greater to naproxen.…”