In vitro activation of NADPH-dependent O2- generating system in a plasma membrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin

Doke, Noriyuki; Miura, Yoshio

doi:10.1006/pmpp.1995.1002

Cited by 77 publications

(36 citation statements)

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“…A Abbreviations: AM, arbuscular mycorrhiza; AMF arbuscular mycorrhizal fungi; APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; GST, glutathione S-transferase; HR, hypersensitive reaction; ROS, reactive oxygen species; SOD, superoxide dismutase. (Auh & Murphy, 1995;Bolwell et al, 1995;Croft et al, 1990;Doke, 1983;Doke & Miura, 1995;Levine et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

et al. 2009

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The bioprotection performance of Piriformospora indica against the root parasite Fusarium verticillioides was studied. We found that maize plants first grown with F. verticillioides and at day 10 inoculated with P. indica showed improvements in biomass, and root length and number as compared with plants grown with F. verticillioides alone. To validate our finding that inoculation with P. indica suppresses colonization by F. verticillioides, we performed PCR analyses using P. indica-and F. verticillioides-specific primers. Our results showed that inoculation with P. indica suppresses further colonization by F. verticillioides. We hypothesized that as the colonization by P. indica increases, the presence of/colonization by F. verticillioides decreases. In roots, catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST) and superoxide dismutase (SOD) activities were found to be higher in F. verticillioides-colonized plants than in noncolonized plants. Increased activity of antioxidant enzymes minimizes the chances of oxidative burst (excessive production of reactive oxygen species), and therefore F. verticillioides might be protected from the oxidative defence system during colonization. We also observed decreased antioxidant enzyme activities in plants first inoculated with F. verticillioides and at day 10 inoculated with P. indica as compared with plants inoculated with F. verticillioides alone. These decreased antioxidant enzyme activities due to the presence of P. indica help the plant to overcome the disease load of F. verticillioides. We propose that P. indica can be used as a bioprotection agent against the root parasite F. verticillioides. INTRODUCTIONMycorrhizal associations are among the most widespread, intimate and important symbioses in terrestrial ecosystems. Among these, arbuscular mycorrhiza (AM) represent the most ancient and widespread mycorrhizal symbiosis, and are found in a wide range of land plant species (Harrison, 1997). The successful establishment of this mutualistic association constitutes a strategy to fulfil the nutritional demands of both partners . This requires a balance between the defence responses of the host plant and the nutrient demands of the endophyte, resulting in an altered defence-related gene expression, which has been extensively studied during host colonization by obligate biotrophic arbuscular mycorrhizal fungi (AMF). Induction of defence-related genes is most prominent during the early stages of colonization (García-Garrido & Ocampo, 2002), but can also be detected during arbuscule development (Grunwald et al., 2004).The active resistance of plants to colonization by fungi is often expressed by the hypersensitive reaction (HR) of challenged plant cells, and is characterized by reactive oxygen species (ROS), as well as by induced rapid and localized death of plant tissue at the site of infection (Ingram, 1978;Tenhaken et al., 1995). At the biochemical level, the rapid generation of ROS, such as superoxide (O { 2 ) and hydrogen peroxide (H 2 O 2 ), kno...

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Section: Introductionmentioning

confidence: 99%

“…A variety of enzyme systems have been proposed to generate ROS in plants. These include a neutrophil-analogous, membrane-bound NADPH oxidase, a lipoxygenase and apoplastic peroxidases (Auh & Murphy, 1995;Bolwell et al, 1995;Croft et al, 1990;Doke, 1983;Doke & Miura, 1995;Levine et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

et al. 2009

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“…The respiratory burst is attributed to the activation of a host plasma membrane-bound NADPH oxidase that transfers electrons from NADPH on the interior of the cell to molecular oxygen on the outside, leading to the generation of 02- (Segal and Abo, 1993). Speculation is that a plasma membrane-localized NADPH oxidase similar to that in the phagocyte is responsible for the oxidative burst in plants (Vera-Estrella et al, 1992;Baker et al, 1993;Lamb, 1994;Mehdy, 1994;Doke and Miura, 1995).…”

Section: Resultsmentioning

confidence: 99%

“…the oxidative burst, may affect the attacking pathogen and the host plant cells at the infection site, thereby limiting the spread of the pathogen (Mehdy, 1994). Speculation on the involvement of a plasma membraneassociated NADPH oxidase in the oxidative burst has been published ( Aposto1 et al, 1989;Vera-Estrella et al, 1992;Baker et al, 1993;Legendre et al, 1993b;Lamb, 1994;Mehdy, 1994;Doke and Miura, 1995). Studies of this enzyme in phagocyte cells indicated that an assembly process involving two plasma membrane subunits and three cytosolic components was triggered in these cells upon pathogen attack (Sega1 and Abo, 1993;Jones, 1994).…”

Section: The Release Of These Active Oxygen Species (Aos) Termedmentioning

confidence: 99%

Race-specific elicitors of Cladosporium fulvum promote translocation of cytosolic components of NADPH oxidase to the plasma membrane of tomato cells.

1997

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The effect of race-specific elicitors on NADPH oxidase was examined in vivo by treating tomato cells with elicitor-containing intercellular fluids prepared from infected tomato leaves inoculated with specific Cladosporium fulvum races. Treatment of Cf-4 or Cf-5 cells with intercellular fluids from incompatible but not from compatible races of C. fulvum increased oxidase activity and the amount of p67-phox, p47-phox, and rac2 in the plasma membrane. Comparison of these three components in the cytosol and plasma membrane indicated that elicitors promoted the translocation of cytosolic components of NADPH oxidase t o the plasma membrane of tomato cells carrying the appropriate resistance gene. Protein kinase C activators and inhibitors did not affect enzyme activity or the binding of these three components t o the plasma membrane. In contrast, staurosporine, calmodulin antagonists, and EGTA inhibited elicitor-induced oxidase activity and the translocation of the cytosolic components. The assembly process involves a Ca 2+-dependent protein kinase that catalyzes the phosphorylation of p67-phox and p47-phox, facilitating their translocation t o the plasma membrane. Our data suggest that although both plants and animals share common elements in eukaryotic signal transduction, the involvement of different protein kinases mediating the activation of phosphorylation of p67-phox and p47-phox may reflect the unique spatial and temporal distribution of signal transduction pathways in plants.

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“…H 2 O 2 is formed by the dismutation of superoxide. Superoxide synthesis has been observed in purified preparations of plasma membrane from several plant systems (Vianello and Macrí, 1989;Qiu et al, 1994;Doke and Miura, 1995;Murphy and Auh, 1996;Van Gestelen et al, 1997).…”

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confidence: 99%

Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

et al. 1998

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In vitro activation of NADPH-dependent O2- generating system in a plasma membrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin

Cited by 77 publications

References 0 publications

Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

Race-specific elicitors of Cladosporium fulvum promote translocation of cytosolic components of NADPH oxidase to the plasma membrane of tomato cells.

Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

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