In vitro activities of non-traditional antimicrobials alone or in combination against multidrug-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii isolated from intensive care units

Timurkaynak, Funda; Can, Füsun; Azap, Özlem Kurt; Demirbilek, Müge; Arslan, Hande; Karaman, Sedef Özbalıkçı

doi:10.1016/j.ijantimicag.2005.10.012

Cited by 196 publications

(148 citation statements)

References 30 publications

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“…Various reports from many countries, such as China, Greece, and Turkey have shown 21.5-84% of A. baumannii isolates during 1996-2009 were MDR A. baumannii and 94-100% of these isolates were susceptible to colistin 11,25,26 . Ninety-eight percent of MDR A. baumannii isolates were susceptible to colistin and had MIC 50 and MIC 90 values of 0.75 and 1 µg/ml, which were similar to those of previous studies from Dizbay 7,8,25 . This indicates that colistin was effective antimicrobial agent against MDR A. baumannii.…”

Section: Discussionsupporting

confidence: 85%

“…Previous in vitro studies have demonstrated that a combination of antimicrobial agents such as colistin and rifampicin, colistin and imipenem, imipenem and rifampicin, and cefoperazone/sulbactam combined with imipenem produce better activity against A. baumannii and MDR A. baumannii [6][7][8] . The combination of antimicrobial agents seems to be an alternative in A. baumannii treatment.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial susceptibility of Acinetobacter baumannii isolated from hospital patients

Tunyapanit¹,

Pruekprasert²,

Laoprasopwattana³

et al. 2014

ScienceAsia

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ABSTRACT:The incidence of multidrug-resistant Acinetobacter baumannii (MDR A. baumannii) is increasing worldwide and is leading to therapeutic problems. We investigated the in vitro activities of cefoperazone/sulbactam, colistin, imipenem, and rifampicin alone and in double combinations against 100 A. baumannii isolates from patients at Songklanagarind Hospital in Songkhla Province, Thailand. The E-test method was used to determine antimicrobial susceptibility, the minimal inhibitory concentration (MIC) and for antimicrobial combination testing. A. baumannii isolates were susceptible to colistin (97%), cefoperazone/sulbactam (69%), imipenem (45%), and rifampicin (13%). Fifty-nine percent of them were MDR A. baumannii. Colistin was superior to cefoperazone/sulbactam, rifampicin and imipenem against MDR A. baumannii and the MIC50, MIC90 of colistin were 0.75 and 1 µg/ml, respectively. Non-MDR A. baumannii isolates were susceptible to cefoperazone/sulbactam (100%), colistin (95%), imipenem (93%) and rifampicin (2%). Combinations of cefoperazone/sulbactam plus colistin or rifampicin, imipenem plus colistin or rifampicin and colistin plus rifampicin showed indifferent effects against most MDR isolates. Of all the antimicrobial combinations tested, cefoperazone/sulbactam plus rifampicin produced the highest percentages (42%) of synergy, partial synergy, and additive results. The activity rate of cefoperazone/sulbactam against MDR A. baumannii was higher when combined with rifampicin than colistin. Thus colistin had the greatest activity against most MDR and non-MDR A. baumannii isolates among all of the antibiotics tested. Cefoperazone/sulbactam and imipenem showed good activity against non-MDR isolates, and cefoperazone/sulbactam combined with rifampicin may be useful in treating infections caused by MDR isolates.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility of Acinetobacter baumannii isolated from hospital patients

Tunyapanit¹,

Pruekprasert²,

Laoprasopwattana³

et al. 2014

ScienceAsia

View full text Add to dashboard Cite

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“…As rifampin was shown to be synergistic with CO or imipenem both in vitro and clinically, we enrolled rifampin as a third agent to try in combination. 1,17,24 The best results were obtained in the TIG-CO combination. Synergistic activity was found in 72% of the isolates with the TIG-CO combination, in 60% with the CO-RIF combination, and in only 12% with the TIG-RIF combination.…”

Section: Discussionmentioning

confidence: 98%

“…Susceptibility to CO was reported as 97.9-100% in various studies. [15][16][17] However, poor pharmacokinetic properties and adverse effects such as nephrotoxicity and neurotoxicity limit its wide clinical usage. 18 Although CO was recommended in salvage therapy of nosocomial MDR-A.…”

Section: Discussionmentioning

confidence: 99%

In vitro synergistic activity of tigecycline and colistin against XDR-Acinetobacter baumannii

et al. 2009

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The emergence of extensive drug-resistant (XDR) Acinetobacter baumannii limits the therapeutic options and leads to high mortality in intensive care units. Combined antibiotic therapy is frequently recommended for the treatment of these infections. Colistin (CO) and tigecycline (TIG), alone or in combination with other antimicrobials, are the most commonly used antibiotics in the treatment of these resistant infections. In this study, the in vitro synergistic activity of TIG and CO were tested for 25 XDR-A. baumannii strains isolated from ventilator-associated pneumonia by the Etest method. Resistance to CO was not detected, whereas 8% of the strains were resistant to TIG. The TIG-CO combination was more synergistic than TIG-rifampin and CO-rifampin according to the fractional inhibitory concentration index. No antagonism was detected between the drugs in the study. There was no strong correlation between the activity of the combinations with reference to strains or genotypes. Our results suggest that the combined use of TIG and CO may be useful for the treatment of XDR-A. baumannii infections.

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“…Synergistic effect resulted from combining cyanidin with antibiotics were assessed by determining fractional inhibitory concentration ( (Timurkaynak et al 2006). Finally, the varying rates of synergy between the agents were determined.…”

Section: Fractional Inhibitory Concentrationmentioning

confidence: 99%

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Gopu

Shetty

2016

J Food Sci Technol

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Quorum sensing (QS) is the process of population dependent cell to cell communication used by bacteria to regulate their phenotypic characteristics. Key virulence factors that determine the bacterial pathogenicity and food spoilage were found to be regulated by QS mechanism. Hence, disrupting the QS signaling pathway could be an attractive strategy to manage food borne pathogens. In the current study, QS inhibitory activity of a naturally occurring anthocyanincyanidin and its anti-biofilm property were assessed against an opportunistic pathogen Klebsiella pneumoniae, using a biosensor strain. Further, QS inhibitory property of a naturally occurring anthocyanin cyanidin was further confirmed using in-silico techniques like molecular docking and molecular dynamics simulation studies. Cyanidin at sub-lethal dose significantly inhibited QS-dependent phenotypes like violacein production (73.96 %), biofilm formation (72.43 %), and exopolysaccharide production (68.65) in a concentrationdependent manner. Cyanidin enhanced the sensitivity of test pathogen to conventional antibiotics in a synergistic manner. Molecular docking analysis revealed that cyanidin binds more rigidly with LasR receptor protein than the signaling compound with a docking score of −9.13 Kcal/mol. Molecular dynamics simulation predicted that QS inhibitory activity occurs through the conformational changes between the receptor and cyanidin complex. Our results indicate that cyanidin, can be a potential QS based antibiofilm and antibacterial agent for food borne pathogens.

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In vitro activities of non-traditional antimicrobials alone or in combination against multidrug-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii isolated from intensive care units

Cited by 196 publications

References 30 publications

Antimicrobial susceptibility of Acinetobacter baumannii isolated from hospital patients

Antimicrobial susceptibility of Acinetobacter baumannii isolated from hospital patients

In vitro synergistic activity of tigecycline and colistin against XDR-Acinetobacter baumannii

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

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