In vitro activity of N-formimidoyl thienamycin (MK0787) against resistant strains of Pseudomonas aeruginosa, Staphylococcus epidermidis, Serratia marcescens, and Enterococcus spp

Abstract

The in vitro activities of N-fonnimidoyl thienamycin (MK0787) and nine other antibiotics were tested against 129 Colonies of isolates to be tested were picked from nutrient agar slants, suspended in 1 ml of MuellerHinton broth, and incubated overnight at 370C. Fifty microliters of a 1:1,000 dilution of this culture in Mueller-Hinton broth was added to each well of the microtiter plate, resulting in final inocula (determined by representative colony counts) of from 4.5 x 104 to 1.0 x 1O colony-forming units per ml. The concentrations of antimicrobial agents in these wells ranged from 0.12 to 100 ug/ml. The MIC was defined as the lowest

In vitro comparison of N-formimidoyl thienamycin (MK0787) and Azlocillin with three aminoglycosides and ticarcillin against Pseudomonas aeruginosa

“…For 24 strains of P. aeruginosa with known mechanisms of resistance for amikacin, tobramycin, or both, all but one strain was inhibited by <8 ,ug/ ml of N-formimidoyl thienamycin (Table 2). Of these 24 strains resistant to azlocillin (MIC 364 ,ug/ml) 14 were susceptible to z 8 ,ug/ml of Nformimidoyl thienamycin.Our data compare closely with published studies which included smaller numbers of P. aeruginosa strains (4,8,9,11,12,15 …”

“…Our data compare closely with published studies which included smaller numbers of P. aeruginosa strains (4,8,9,11,12,15 …”

“…Our data compare closely with published studies which included smaller numbers of P. aeruginosa strains (4,8,9,11,12,15). Of special interest in the present study was the activity of N-formimidoyl thienamycin against strains of P. aeruginosa resistant to one, more than one, or all of the reference antibiotics used in this study.…”

Comparative imipenem treatment of Staphylococcus aureus endocarditis in the rat

“…aureus and Staph. 79 0305-7453/83/12D079 + 09 S02.00/0 © 1983 The British Society for Antimicrobial Chemotherapy epidermidis, including some methicillin-resistant strains (Cherubin et al, 1981;Livingston et al, 1981;Thompson et al, 1982) justified in-vivo studies of this drug in infections caused by these organisms. We selected a model of left-sided Staph.…”

“…Its spectrum of action is unusually broad, with important in-vitro activity against the majority of bacteria often creating therapeutic problems, such as (Cherubin et al 1981;Kesado, Hashizume & Ashai, 1980;Kesado et al, 1982;Livingston, Elliott & Cobbs, 1981;Michael, Alford & McGee, 1981;Thompson, Fischer & Wenzel, 1982;Verbist & Verhaegen, 1981). Only a small incidence of crossresistance to penicillins and cephalosporins has been demonstrated with imipenem.…”