In vitro activity of propolis against Streptococcus pyogenes

Bosio, K.; Avanzini, Claudio; D’Avolio, Antonio; Ozino, O. I.; Savoia, Dianella

doi:10.1046/j.1365-2672.2000.00785.x

Cited by 131 publications

(108 citation statements)

References 16 publications

(25 reference statements)

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“…Propolis has been shown to kill Bacillus larvae, the most important bacterial disease of bees (20). Flavonoids such as Chrysin and Quercetin account for much of the biological activity in propolis.…”

Section: Discussionmentioning

confidence: 99%

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Kapur²,

Jm³

et al. 2007

Int J Oncol

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Abstract. Propolis is a resinous substance collected by bees (Apis mellifera) from various tree buds which they then use to coat hive parts and to seal cracks and crevices in the hive. Propolis, a known ancient folk medicine, has been extensively used in diet to improve health and to prevent disease. In the present study, we have evaluated the effects of ethanolic extracts of Brazilian propolis group l2 and bud resins of botanical origin (B. dracunculifolia), and propolis group 3 on proliferation of metastasis (DU145 and PC-3) and primary malignant tumor (RC58T/h/SA#4)-derived human prostate cancer cells. The strongest inhibition was observed in propolis group 3 (sample #3) extracts whereas moderate growth inhibition was observed in human prostate epithelial cells. In the RC58T/h/SA#4 cells, resins of botanical origin of propolis group 12 (sample #1) and propolis group 12 (sample #2) induced growth inhibition that was associated with S phase arrest whereas propolis group 3 (sample #3) induced growth inhibition that was associated with G2 arrest. The mechanisms of cell cycle effects of propolis were investigated. The resins of botanical origin of propolis group 12 and propolis group 12 showed similar inhibition of cyclin D1, CDK4 and cyclin B1 expression. Propolis group 3 showed higher induction of p21 expression but no inhibition of cyclin D1, CDK4 and cyclin B1 expression. The results obtained here demonstrate that the Brazilian propolis extracts have significant inhibitory effect on proliferation of human prostate cancer cells. Inhibition was achieved through regulation of protein expression of cyclin D1, B1 and cyclin dependent kinase (CDK) as well as p21. Our results indicate that the Brazilian propolis extracts show promise as chemotherapeutic agents as well as preventive agents against prostate cancer. IntroductionProstate cancer is the most common male cancer in USA and other Western countries, and the second leading cause of male cancer death in USA (1). Despite its pervasive impact, the etiology of prostate cancer and the factors that promote its progression are not well understood. To date, there is no secure way to tell whether prostate cancer, once found, should receive treatment. Although prostatectomy, radiation therapy and hormone therapy have been used for curing prostate cancer patients, recurrence and metastasis of prostate cancer are major problems in prostate cancer therapy. Current available treatment often has troubling side-effects such as urinary incontinence and erectile dysfunction. There is no effective treatment modality once the cancer has evolved into the hormone refractory stage. Preventive strategies are currently emerging. These is an increase in the usage of nutritional supplements such as soybeans, garlic, green tea and Vitamin D3 etc., to augment the prescribed anticancer therapies.Propolis is a resinous substance collected by Apis mellifera from various tree buds which they then use to coat hive parts and to seal cracks and crevices in the hive (2). Propolis has been used as ...

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Section: Discussionmentioning

confidence: 99%

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Kapur²,

Jm³

et al. 2007

Int J Oncol

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“…This test was apparently not influenced by the solubility of the active components in the broth. Using serial dilution in tubes for European propolis, MBC of 1.5 mg/ mL for S. aureus (14) and of 3.0 mg/mL for S. aureus and S. epidermidis (8) Bosio et al (4) used both dilution and diffusion methods to study the activity of Italian propolis against S. pyogenes concluding that, whereas the results of the agar diffusion tests with disks were not suitable for comparing specimens, the agar dilution tests gave results that corresponded to the results of the HPLC tests.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

Sawaya¹,

Souza²,

Marcucci³

et al. 2004

Braz. J. Microbiol.

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Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of the extracts ranging between 2.5 and 20.0 mg/mL, for the species of Gram-positive bacteria tested. The results of the Agar Plate Diffusion were directly proportional to the hydro-solubility of the extracts and did not evaluate their bactericidal activity correctly. The bactericidal activity of this sample of propolis was due to the combined effect of several components that were identified by HPLC and were best extracted using 50% ethanol as a solvent.

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“…Owing the ability to synthesize many different substances, the propolis is one of the top richest sources of new drugs (1,11). It is showed that ethanol extract of propolis has a high potential as an alternative source of antibacterial compounds (2,3,4,5,13,19,20,22,33).…”

Section: Introductionmentioning

confidence: 99%

In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae)

Tukmechi¹,

Ownagh²,

Mohebbat³

2010

Braz. J. Microbiol.

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The ''in vitro'' antibacterial activity of ethanol extract of propolis (EEIP) from Urmia, Iran was investigated against three prevalent species of fish bacterial pathogens including: Aeromonas hydrophila LMG 3770, Yersinia ruckeri LMG 3279 and Streptococcus iniae LMG 14520. In this study two standard susceptibility testing techniques (Micro-broth dilution method and Agar-well diffusion method) were used to evaluation of the antibacterial activity of EEIP against the mentioned micro-organisms. Also the chemical composition of propolis was determined by the method of Gas chromatography-mass spectrometry (GC-MS). Twenty-six compounds were identified by gas chromatography-mass spectrometry analysis. Results showed Chemical composition of EEIP contained significant amounts of flavonoids, Sesquiterpenes -mainly Eudesmol and Caryophyllene oxide -aromatic acid, and low amounts of aldehydes and triterpens. Furthermore the ethanol extract of propolis inhibited the growth of all examined micro-organisms with the highest antimicrobial activity against Gram-positive bacteria Streptococcus iniae. Ethanol did not influence the antimicrobial effect of EEIP. These antibacterial properties would warrant further studies on the clinical applications of propolis in aquaculture field.

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In vitro activity of propolis against Streptococcus pyogenes

Cited by 131 publications

References 16 publications

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae)

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