IN VITRO ALTERNATIVE IRRITANCY ASSAYS: COMPARISON OF CYTOTOXIC and MEMBRANE TRANSPORT EFFECTS OF ALCOHOLS*

Borenfreund, Ellen; Shopsis, Charles; Borrero, Olina; Sathe, Sonal

doi:10.1111/j.1749-6632.1983.tb47849.x

Cited by 7 publications

References 2 publications

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Dynamic aspects of rhodamine dye photosensitization in vitro with an argon‐ion Laser

Shea¹,

Chen²,

Hasan³

1989

Lasers Surg Med

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Phototoxicity in cultured human bladder carcinoma cells treated by 514.5-nm argon-ion laser irradiation plus rhodamine-123 (R123) or tetrabromo-R123 (TBR) was assessed counting total cell number and percent viability at 1, 24, 48, 72, and 96 h after irradiation. TBR was markedly more efficient than R123 at causing both reversible and persistent phototoxic cytostasis. Furthermore, TBR photosensitization caused net cytocidal effects at 0.5 J/cm2, which were not seen with R123 photosensitization at up to 40 J/cm2. The reduced phototoxic efficiency of R123 as compared to TBR appears, in part, to reflect the presence of a fraction of cells refractory to R123 photosensitization.

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Dynamic aspects of rhodamine dye photosensitization in vitro with an argon‐ion Laser

Shea¹,

Chen²,

Hasan³

1989

Lasers Surg Med

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In Vitro Biochemical Markers of Skin Toxicity

Galli

Marinovich

1990

Skin Pharmacology and Toxicology

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In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test

1984

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A short-term cytotoxicity assay carried out in multiwell test plates and a supplementary colony forming assay are both useful for screening and range finding of toxic concentrations of test agents. The highest tolerated dose (HTD), a concentration at which only minimal morphological changes were observed, was designated as endpoint in the assay. Epithelial rabbit cornea cells, murine fibroblasts, Chinese hamster lung cells, human hepatoma cells and mouse macrophage cultures were used as targets. Several of the alcohols tested at HTD in the colony forming assay were found to inhibit colony formation. An ID50 of colony formation was used as a quantitative corroborating test. The ranking of 34 toxicants was found to be virtually the same with all cell types examined. This easily reproducible, rapid in vitro test is cost-effective and can be used for preliminary large scale screening of potential toxicants.

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IN VITRO ALTERNATIVE IRRITANCY ASSAYS: COMPARISON OF CYTOTOXIC and MEMBRANE TRANSPORT EFFECTS OF ALCOHOLS*

Cited by 7 publications

References 2 publications

Dynamic aspects of rhodamine dye photosensitization in vitro with an argon‐ion Laser

Dynamic aspects of rhodamine dye photosensitization in vitro with an argon‐ion Laser

In Vitro Biochemical Markers of Skin Toxicity

In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test

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