In vitro and in situ intercellular adhesion molecule-1 (ICAM-1) expression by endothelial cells lining a polyester fabric

Rémy, Murielle; Valli, Nathalie; Brèthes, Daniel; Labrugère, Christine; Durrieu, Marie‐Christine; Dobrova, N. B.; Novikova, S. P.; Gorodkov, A. Yu.; Bordenave, Laurence

doi:10.1016/s0142-9612(98)00167-7

Cited by 22 publications

(16 citation statements)

References 32 publications

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“…5). Scatchard analysis revealed ϳ1.6 ϫ 10 6 binding sites for antiICAMs [which agrees well with previous reports by other groups (Remy et al, 1999)] and ϳ300 binding sites for anti-ICAM/PNCs per cell (insets in Fig. 5).…”

Section: Resultssupporting

confidence: 91%

Endothelial Targeting of High-Affinity Multivalent Polymer Nanocarriers Directed to Intercellular Adhesion Molecule 1

Muro

Dziubla²,

Qiu³

et al. 2006

J Pharmacol Exp Ther

179

278

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Targeting of diagnostic and therapeutic agents to endothelial cells (ECs) provides an avenue to improve treatment of many maladies. For example, intercellular adhesion molecule 1 (ICAM-1), a constitutive endothelial cell adhesion molecule upregulated in many diseases, is a good determinant for endothelial targeting of therapeutic enzymes and polymer nanocarriers (PNCs) conjugated with anti-ICAM (anti-ICAM/PNCs). However, intrinsic and extrinsic factors that control targeting of anti-ICAM/PNCs to ECs (e.g., anti-ICAM affinity and PNC valency and flow) have not been defined. In this study we tested in vitro and in vivo parameters of targeting to ECs of anti-ICAM/ PNCs consisting of either prototype polystyrene or biodegradable poly(lactic-coglycolic) acid polymers (ϳ200 nm diameter spheres carrying ϳ200 anti-ICAM molecules). Anti-ICAM/ PNCs, but not control IgG/PNCs 1) rapidly (t 1/2 ϳ5 min) and specifically bound to tumor necrosis factor-activated ECs in a dose-dependent manner (B max ϳ350 PNC/cell) at both static and physiological shear stress conditions and 2) bound to ECs and accumulated in the pulmonary vasculature after i.v. injection in mice. Anti-ICAM/PNCs displayed markedly higher EC affinity versus naked anti-ICAM (K d ϳ80 pM versus ϳ8 nM) in cell culture and, probably because of this factor, higher value (185.3 Ϯ 24.2 versus 50.5 Ϯ 1.5% injected dose/g) and selectivity (lung/blood ratio 81.0 Ϯ 10.9 versus 2.1 Ϯ 0.02, in part due to faster blood clearance) of pulmonary targeting. These results 1) show that reformatting monomolecular anti-ICAM into high-affinity multivalent PNCs boosts their vascular immunotargeting, which withstands physiological hydrodynamics and 2) support potential anti-ICAM/PNCs utility for medical applications.

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Section: Resultssupporting

confidence: 91%

Endothelial Targeting of High-Affinity Multivalent Polymer Nanocarriers Directed to Intercellular Adhesion Molecule 1

Muro

Dziubla²,

Qiu³

et al. 2006

J Pharmacol Exp Ther

179

278

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“…At present, polyester fiber is being used for artificial blood vessels. Several studies have reported that chitosan possesses the ability to adhere to the polyester fiber with some modification [14][15][16]. Thus, the CS/CNTs composite films would be highpotential candidates for artificial blood vessel coating materials.…”

Section: Discussionmentioning

confidence: 99%

Improved blood biocompatibility of composite film of chitosan/carbon nanotubes complex

et al. 2009

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Single-walled carbon nanotubes are novel molecular-scale wires having excellent anti-adhesion properties with regard to platelets. On the other hand, chitosan is a partially de-acetylated derivative of chitin that has a critical role in cell attachment and growth. The aim of this study was to investigate how carbon nanotubes improve the blood biocompatibility of chitosan film. We prepared composite films with various concentrations of chitosan/carbon nanotubes (CS/CNTs) (1.3-6.3 wt%). The sample surfaces were characterized by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and contact angle measurements. The surface characterization revealed that the surface of the CS/CNTs composite film became more hydrophobic with increasing amounts of CNTs. Cell attachment tests using bovine aortic endothelial cells (BAECs) indicated that CS/ CNTs composite films retained their cell adhesion ability. The blood compatibility of the CS/CNTs composite films was evaluated using the blood platelet adhesion and activation tests in vitro. Platelet adhesion results confirmed that platelet adhesion and the formation of a platelet network were inhibited on composite films with higher concentrations of CNTs (5.1 wt%). Our experimental results show that the novel composite film containing CS/CNTs possesses two paradoxical characteristics, namely, good adherence of endothelial cells and minimum adherence and activation of platelets, making this film a promising antithrombogenic material for use in the biomedical field.

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“…ICAM-1, a cell-surface glycoprotein member of the Ig super-family is expressed by normal vascular endothelium at a basal level of surface density 23,24 that markedly increases under pathological conditions including inflammation as a result of endothelial activation. [23][24][25] BAOECs were subjected to 24 h or more of flow at 12 dyn/cm 2 and were finally activated with TNF-a to study surface ICAM-1 expression. TNF-a was mixed in media at a concentration of 10 Units (U)/ml, and the treatment was performed for 2 h on a confluent endothelial cell layer (>80%).…”

Section: Tnf-a Treatmentmentioning

confidence: 99%

Biomimetic channel modeling local vascular dynamics of pro-inflammatory endothelial changes

et al. 2016

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Endothelial cells form the inner lining of blood vessels and are exposed to various factors like hemodynamic conditions (shear stress, laminar, and turbulent flow), biochemical signals (cytokines), and communication with other cell types (smooth muscle cells, monocytes, platelets, etc.). Blood vessel functions are regulated by interactions among these factors. The occurrence of a pathological condition would lead to localized upregulation of cell adhesion molecules on the endothelial lining of the blood vessel. This process is promoted by circulating cytokines such as tumor necrosis factor-alpha, which leads to expression of intercellular adhesion molecule-1 (ICAM-1) on the endothelial cell surface among other molecules. ICAM-1 is critical in regulating endothelial cell layer dynamic integrity and cytoskeletal remodeling and also mediates direct cell-cell interactions as part of inflammatory responses and wound healing. In this study, we developed a biomimetic blood vessel model by culturing confluent, flow aligned, endothelial cells in a microfluidic platform, and performed real time in situ characterization of flow mediated localized pro-inflammatory endothelial activation. The model mimics the physiological phenomenon of cytokine activation of endothelium from the tissue side and studies the heterogeneity in localized surface ICAM-1 expression and F-actin arrangement. Fluorescent antibody coated particles were used as imaging probes for identifying endothelial cell surface ICAM-1 expression. The binding properties of particles were evaluated under flow for two different particle sizes and antibody coating densities. This allowed the investigation of spatial resolution and accessibility of ICAM-1 molecules expressed on the endothelial cells, along with their sensitivity in receptor-ligand recognition and binding. This work has developed an in vitro blood vessel model that can integrate various heterogeneous factors to effectively mimic a complex endothelial microenvironment and can be potentially applied for relevant blood vessel mechanobiology studies. V C 2016 AIP Publishing LLC. [http://dx

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In vitro and in situ intercellular adhesion molecule-1 (ICAM-1) expression by endothelial cells lining a polyester fabric

Cited by 22 publications

References 32 publications

Endothelial Targeting of High-Affinity Multivalent Polymer Nanocarriers Directed to Intercellular Adhesion Molecule 1

Endothelial Targeting of High-Affinity Multivalent Polymer Nanocarriers Directed to Intercellular Adhesion Molecule 1

Improved blood biocompatibility of composite film of chitosan/carbon nanotubes complex

Biomimetic channel modeling local vascular dynamics of pro-inflammatory endothelial changes

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