In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella

Abstract

Punica granatum is commonly used in Korea as a traditional medicine for the treatment of pathogenic bacteria. In this study, we investigated the in vitro and in vivo antimicrobial activity of P. granatum peel EtOH extract (PGPE) against 16 strains of Salmonella. The minimal inhibitory concentrations of PGPE were in the range of 62.5–1000 x03BCg mL−1. In addition, the in vivo antibacterial activity of the PGPE extract was examined in a S. typhimurium infection mouse model. Mice were initially infected with S. typhimurium and then with PGPE. The extract was found to have significant effects on mortality and the numbers of viable S. typhimurium recovered from feces. Although clinical signs and histological damage were rarely observed in the treated mice, the untreated controls showed signs of lethargy and histological damage in the liver and spleen. Taken together, the results of this study indicate that PGPE has the potential to provide an effective treatment for salmonellosis.

In vitro antifungal activity of indirubin isolated from a South Indian ethnomedicinal plant Wrightia tinctoria R. Br.

“…The methanol extract of Punica granatum fruit rind showed dermatophytic activity at low concentrations. Choi et al (2009) reported anti-salmonella activity of Punica granatum ethanol extract.…”

Polyphenol Extracts from <i>Punica granatum</i> and <i>Terminalia chebula</i> Are Anti-inflammatory and Increase the Survival Rate of Chickens Challenged with <i>Escherichia coli</i>

“…The polyphenol content is 25-30% in P. granatum, and 23-37% in T. chebula RETZ. 24) Their extracts have shown multiple pharmacological activities, such as antimicrobial, 8,15,25) cardiac action, antioxidant, 11,26) antitumor, 10,27,28) antiviral, 29) and the promotion of wound healing. 12,13) The intestinal tract contains the largest reservoir of bacteria and endotoxins in an animal's body.…”

Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel

“…Parekh et al mentioned that plants extracts from organic solvent have been found to give more consistent antimicrobial activity compared to water extract [29] . Recently, many research studies have been done to discover the presence of various bioactive compounds like flavonoids, tannins, phenol in fruits peels like mangosteen, pomegranate, citrus, and grape [30][31][32][33][34] . However, there are no reports available on the antibacterial activities of the tropical pumpkin.…”