In vitro and in vivo studies of N,N′-bis[2(2-pyridyl)-methyl]pyridine-2,6-dicarboxamide–copper(II) and rheumatoid arthritis

“…Administration of the agents was performed intravenously through the tail vein and percutaneously on the anterior dorsal side of the mice. Synthesis of peptides mimics ( Figure 1) in this study has been reported in our previous studies [10,11]. Cu(II) complexes of…”

Section: Introductionmentioning

confidence: 77%

“…-pyridine-dicarboxamide (L3) characterized from our previous studies, were prepared in sterile saline solution at pH 7.4 [10,11]. These solutions were spiked with 10-14m Ci …”

Section: Animalsmentioning

confidence: 99%

In Vivo Bio-Distribution Study of 64Cu (II)-Labelled Copper (II) Complexes of Peptides Mimics in Balb/C Mice-Development of Copper Based Anti-Inflammatory Agents

Odisitse

Jackson

2017

MOJBOC

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Bio-distribution of 64Cu-labelled copper(II) complexes of N,N'-di(aminoethylene)-2,6-pyridinedicarbonylamine, Bis-(N,N-dimethylethyl)-2,6-pyridinedicarboxamide and N,N'-bis[2(2-pyridyl)-methyl]pyridine-2,6-pyridinedicarboxamide was determined using Balb/c mice. Radio labelled copper (II) complexes of peptides mimics were injected in animals intravenously via tail vein and by applying on anterior dorsal side. After 1, 6 and 24 hour post-injection animals were sacrificed and organs or samples removed for measuring activity using gamma counter. Compounds accumulated in various parts of the body 1, 6 and 24 hour post administration. Intravenous and dermal absorption studies showed that these compounds have 50% retention in the body 24 hours after administration.

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“…[8] Copper is a biologically pertinent element, with its compounds having shown special biochemical and spectroscopic attributes, including cleavage and DNA binding, antibacterial, anti-oxidant, antineoplastic, and anti-inflammatory attributes. [9][10][11][12][13][14] Besides, cancer cell membranes are selectively permeable to Cu(II) and are active in hypoxic situations. [15,16] The features of Copper (II) compounds are tightly correlated with the essence of donor atoms and ligands bound to the ion.…”

Section: Introductionmentioning

confidence: 99%

In‐silico and In‐vitro Studies on the DNA/BSA‐Binding Features of a Mixed Ligand Copper (II) Complex Containing 2‐Methyl Imidazole and a Schiff Base Ligand

et al. 2019

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The study aimed at investigating the binding features of the mixed ligand Copper (II) complex to BSA and DNA. Both in‐silico and in‐vitro analyses were made to fully assess the binding mechanism and mode of the Copper (II) complex to BSA and DNA. Spectroscopic tests demonstrated the Copper (II) complex reacted with DNA through binding to its minor grooves with the association constant 3.36×103 M−1. This type of interaction was confirmed by molecular docking simulations. Tryptophan quenching analyses at 298, 303 and 308 K showed that the Copper (II) complex reacted with the BSA's ground‐state fluorophore and created a non‐fluorescent adduct. In other words, a static quenching approach was offered with the quenching rate constants within the span of 3.4×1012‐1.28×1013 M−1s−1. Besides, the values of binding constants at various temperatures were achieved, implying the moderate similarity of the Copper (II) complex to the serum's albumin. Research on thermodynamic factors suggested the complex‐BSA bond was an enthalpy‐induced reaction where Van der Waals forces and the hydrogen bond performed the leading role. The simulations of molecular docking verified the test results and demonstrated the amino acids engaged in the bond between BSA and the Copper (II) complex.

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In vitro and in vivo studies of N,N′-bis[2(2-pyridyl)-methyl]pyridine-2,6-dicarboxamide–copper(II) and rheumatoid arthritis

Cited by 17 publications

References 43 publications

New measurement and evaluation of the excitation function of 64Ni(p,n) reaction for the production of 64Cu

New measurement and evaluation of the excitation function of 64Ni(p,n) reaction for the production of 64Cu

In Vivo Bio-Distribution Study of 64Cu (II)-Labelled Copper (II) Complexes of Peptides Mimics in Balb/C Mice-Development of Copper Based Anti-Inflammatory Agents

In‐silico and In‐vitro Studies on the DNA/BSA‐Binding Features of a Mixed Ligand Copper (II) Complex Containing 2‐Methyl Imidazole and a Schiff Base Ligand

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