In vitro and in vivo Antidiabetic and Antiglycation Properties of Apium graveolens in Type 1 and 2 Diabetic Rat

Gutierrez, Rosa Martha Pérez; Juarez, V; Sauceda, Jahel Valdés; Sosa, Irasema Anaya

doi:10.3923/ijp.2014.368.379

Cited by 15 publications

(10 citation statements)

References 20 publications

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“…To determine the antiglycation activity, the method of [ 17 ] was followed with some modifications. The extract containing 0.02% sodium azide was incubated at 37°C, with BSA (10mg/ml) and 500mM of phosphate buffer for a final volume of 5 ml at pH 7.4, with a final concentration of 2mg/ml of BSA, 40 mM glucose, and 0.30-5mg/ml CFH dissolved in DMSO.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity and In Vitro Antiglycation of the Fruit of Spondias purpurea

Muñiz

Garcia

González

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Hyperglycemia in diabetes mellitus causes irreversible life-threatening micro- and macrovascular complications. There is evidence that the glycation reaction leads to a chemical modification of the proteins contributing to the complications of diabetes. It is known that advanced glycation end products (AGEs) are formed by glycation and oxidation reactions called glycoxidation. CML, a nonfluorescent AGE, has become a biomarker of glycoxidative damage; other AGEs appear to induce oxidative stress, which results in cytotoxicity. To determine antioxidant activity, the FRAP, DPPH, and TEAC tests were used, as well as the polyphenols content using Folin-Ciocalteu's method. To evaluate the antiglycation activity, the BSA/glucose system was used, and the fructosamine concentration, protein carbonyl content, thiol, and CML groups were determined. The results obtained show that the hexane extract of the fruit of Spondias purpurea (CFH) effectively inhibits the glycation reaction, in addition to increasing the thiol groups and decreasing levels of fructosamine, protein carbonyl, and CML. In addition, CFH presented significant antioxidant activity. CFH inhibits the glycation reaction; therefore, it can help prevent complications related to AGEs in diabetes mellitus; it also reduces oxidative stress and is effective in protecting proteins from oxidative damage.

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Section: Methodsmentioning

confidence: 99%

Antioxidant Activity and In Vitro Antiglycation of the Fruit of Spondias purpurea

Muñiz

Garcia

González

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…The antioxidant properties of this plant that prevented the oxygen species activity and reduced the oxidative stress may be a justification for this result. Gutierrez et al indicated that administering A. graveolens extract could decrease the level of FBS in plasma of the diabetic rat(19). The decline in insulin level by A. graveolens extract may stem from the presence of compounds such as luteolin flavonoids and other compounds such as apoysyl glucoside 7-O., Apigenin 7-O apysyl glucoside, and Cryosurial 7-O apysyl glucoside…”

mentioning

confidence: 99%

Investigating the Role of Hydroalcoholic Extract of Apium graveolens and Cinnamon zeylanicum on Metabolically Change and Ovarian Oxidative Injury in a Rat Model of Polycystic Ovary Syndrome

Khodaeifar

Fazljou

Khaki

et al. 2018

International Journal of Women’s Health and Reproduction Scienc

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Objectives: The present study aimed to compare the role of the hydroalcoholic extract of Apium graveolens and Cinnamon zeylanicum on metabolically change and ovarian oxidative injury in a rat model of polycystic ovary syndrome (PCOS). Materials and Methods: In this experimental research, 64 female Wistar rats with breeding were used including the following groups (G): (I) G1: healthy control; (II) G2: PCOS which received no therapy; (III) G3: PCOS + A. graveolens; (IV) G4: A. graveolens; (V) G5: PCOS + C. zeylanicum; (VI) G6: no PCOS + C. zeylanicum; (VII) G7: PCOS + C. zeylanicum and A. graveolens; and (VIII) G8: C. zeylanicum and A. graveolens. The PCOS was induced by a single dose of the intramuscularly injected estradiol valerate (16 mg/ kg). After 14 days, the animals were anesthetized, then their plasma samples were used to check the blood sugar (BS), insulin, and lipid profile. The ovaries of the rats were removed and fixed for histopathological assessment. In addition, the oxidative stress marker in ovarian tissue was evaluated. Results: The levels of BS, insulin, and lipid profile in plasma significantly enhanced in G2 (P < 0.05) while decreasing significantly in the therapy groups, (P < 0.05). Moreover, a significant decline was observed in the serum level of high-density lipoprotein (HDL) in G2 (P < 0.05) while it enhanced significantly in the therapeutic animals (P < 0.05). Furthermore, a negative change was found in the PCOS group on the ovarian tissue. Besides the oxidative stress enhanced in this tissue while in the treated groups this change was improved. Conclusions: Generally, it was revealed that the extract of A. graveolens and C. zeylanicum had a useful impact on regulating the serum levels of fast blood sugar (FBS), insulin, lipid profile, and oxidative stress markers in the palliation of the PCOS complications.

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“…25 - 28 Celery has some potent hypoglycemic agents, such as essential oils, phenolics, triterpenes, and flavonoids (kaempferol, quercetin, triterpenes and luteolin), which possess anti-diabetic activity. 29 Flavonoids act as anti-diabetics by reducing apoptosis, increasing pancreatic beta cell proliferation, stimulating insulin secretion, controlling glucose metabolism in the liver and decreasing hyperglycemia. 29 They also play a role in decreasing insulin resistance, decreasing inflammation in adiposity cells, and inhibiting oxidative stress in skeletal muscles.…”

Section: Discussionmentioning

confidence: 99%

“… 29 Flavonoids act as anti-diabetics by reducing apoptosis, increasing pancreatic beta cell proliferation, stimulating insulin secretion, controlling glucose metabolism in the liver and decreasing hyperglycemia. 29 They also play a role in decreasing insulin resistance, decreasing inflammation in adiposity cells, and inhibiting oxidative stress in skeletal muscles. 30 Kaempferol acts as an anti-diabetic due to its action in pancreatic beta cell protection, a function associated with type 2 diabetes.…”

Section: Discussionmentioning

confidence: 99%

The effects of celery leaf (apium graveolens L.) treatment on blood glucose and insulin levels in elderly pre-diabetics

Yusni

Zufry

Meutia

et al. 2018

SMJ

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Objectives:To analyze the effect of celery leaf extract on blood glucose and plasma insulin levels in elderly pre-diabetics.Methods:This study was conducted between March and November 2014 at the Faculty of Medicine, Syiah Kuala University, Banda Aceh, Indonesia. A quasi-experimental pretest-posttest with a control group was conducted with elderly pre-diabetic volunteers. The subjects included 16 elderly pre-diabetics older than 60 (6 males and 10 females). The subjects were randomly divided into 2 groups: a control group (placebo-treated) and a treatment group (celery-treated). The treatment consisted of celery leaf extract capsules at the dose of 250 mg, 3 times per day (morning, afternoon and evening), 30 minutes before a meal, for 12 days. Data analysis was performed using the t-test (p<0.05).Results:There was a significant decrease in pre-prandial plasma glucose levels (p=0.01) and post-prandial plasma glucose levels (p=0.00), but no significant increase in plasma insulin levels (p=0.15) after celery leaf treatment in elderly pre-diabetics.Conclusion:Celery was effective at reducing blood glucose levels, but there was a lack of association between blood glucose levels and plasma insulin levels in elderly pre-diabetics.

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In vitro and in vivo Antidiabetic and Antiglycation Properties of Apium graveolens in Type 1 and 2 Diabetic Rat

Cited by 15 publications

References 20 publications

Antioxidant Activity and In Vitro Antiglycation of the Fruit of Spondias purpurea

Antioxidant Activity and In Vitro Antiglycation of the Fruit of Spondias purpurea

Investigating the Role of Hydroalcoholic Extract of Apium graveolens and Cinnamon zeylanicum on Metabolically Change and Ovarian Oxidative Injury in a Rat Model of Polycystic Ovary Syndrome

The effects of celery leaf (apium graveolens L.) treatment on blood glucose and insulin levels in elderly pre-diabetics

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