In vitro and in vivo mechanism of immunomodulatory and antiviral activity of Edible Bird's Nest (EBN) against influenza A virus (IAV) infection

Haghani, Amin; Mehrbod, Parvaneh; Safi, Nikoo; Aminuddin, Nur Ain; Bahadoran, Azadeh; Omar, Abdul Rahman; Ideris, Aini

doi:10.1016/j.jep.2016.03.020

Cited by 65 publications

(62 citation statements)

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“…The modification and manupulation of cytokines production is a critical step in influenza pathogenesis, which can recruit a variety of innate immune cells [59]. In this study, in completing our previous study [54,60], interleukin-6 (IL-6) and chemokine C-C motif ligand 2 (CCL-2) from the category of pro-inflammatory cytokines and interferon-β (IFN-β) from the category of anti-inflammatory cytokines were tested at the genome and protein levels.…”

Section: Discussionmentioning

confidence: 77%

“…Chemokine (C-C motif) ligand 2 (CCL-2) was one of the affected chemokines evaluated in this study. This chemokine recruits memory T cells, exudate macrophages (exMACs) and monocytes to the site of the infection [60,63,64]. Attraction of these CCR2 + inflammatory cells to the site would lead to naive T cell proliferation, NO synthesis 2 (NOS2) and TNF-α production [63]; however, the extreme recruitment of these cells causes extra cytokine production and apoptosis induction by tumor necrosis factor-related apoptosis inducing legend (TRAIL) activation [65].…”

Section: Discussionmentioning

confidence: 99%

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Experimental validation and computational modeling of anti-influenza effects of quercetin-3-O-α-L-rhamnopyranoside from indigenous south African medicinal plant Rapanea melanophloeos

Mehrbod

Ebrahimi

Fotouhi

et al. 2019

BMC Complement Altern Med

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Background: Influenza A virus (IAV) is still a major health threat. The clinical manifestations of this infection are related to immune dysregulation, which causes morbidity and mortality. The usage of traditional medication with immunomodulatory properties against influenza infection has been increased recently. Our previous study showed antiviral activity of quercetin-3-O-α-L-rhamnopyranoside (Q3R) isolated from Rapanea melanophloeos (RM) (L.) Mez (family Myrsinaceae) against H1N1 (A/PR/8/34) infection. This study aimed to confirm the wider range of immunomodulatory effect of Q3R on selective pro-and anti-inflammatory cytokines against IAV in vitro, to evaluate the effect of Q3R on apoptosis pathway in combination with H1N1, also to assess the physical interaction of Q3R with virus glycoproteins and RhoA protein using computational docking. Methods: MDCK cells were exposed to Q3R and 100CCID 50 /100 μl of H1N1 in combined treatments (co-, pre-and post-penetration treatments). The treatments were tested for the cytokines evaluation at RNA and protein levels by qPCR and ELISA, respectively. In another set of treatment, apoptosis was examined by detecting RhoA GTPase protein and caspase-3 activity. Molecular docking was used as a tool for evaluation of the potential anti-influenza activity of Q3R. Results: The expressions of cytokines in both genome and protein levels were significantly affected by Q3R treatment. It was shown that Q3R was much more effective against influenza when it was applied in co-penetration treatment. Q3R in combination with H1N1 increased caspase-3 activity while decreasing RhoA activation. The molecular docking results showed strong binding ability of Q3R with M2 transmembrane, Neuraminidase of 2009 pandemic H1N1, N1 and H1 of PR/8/1934 and Human RhoA proteins, with docking energy of − 10.81, − 10.47, − 9.52, − 9.24 and − 8.78 Kcal/mol, respectively.(Continued on next page)

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Experimental validation and computational modeling of anti-influenza effects of quercetin-3-O-α-L-rhamnopyranoside from indigenous south African medicinal plant Rapanea melanophloeos

Mehrbod

Ebrahimi

Fotouhi

et al. 2019

BMC Complement Altern Med

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“…Of several Chinese medicine regimens, the use of the edible bird's nest (EBN) has been widely proposed for its possible effectiveness against new and emerging influenzas, and its utility has been extensively studied. Haghani et al studied the "in vitro and in vivo mechanism of immunomodulatory and antiviral activity of EBN against influenza A virus infection [9] . Immunomodulation via cytokine induction due to EBN has been reported [10] .…”

Section: Chinese Medicine For Emerging Influenzamentioning

confidence: 99%

Chinese Medicine in the Management of New and Emerging Infectious Diseases

Wiwanitkit

2018

Digital Chinese Medicine

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“…As many studies have characterized functional bioactive peptides from a variety source of proteins [9,10] , the similar method of studies was applied to explore the benefits of consuming EBN in the form of soup, supplements, food products, and ingredients. [6] The EBN was proven scientifically to possess high medicinal benefits in enhancing complexion, strengthening immune system [11] , stimulating epidermal growth [7] , depressing the production of TNF-α [12] , inhibiting viral infection [13] , improving respiratory and digestive problems. [14][15][16] Incorporating EBN into food products can improve the variety, quality, taste, and availability of the EBN products for consumption.…”

Section: Introductionmentioning

confidence: 99%

Edible Bird’s Nest: Physicochemical Properties, Production, and Application of Bioactive Extracts and Glycopeptides

Daud

Yusop

Babji

et al. 2019

Food Reviews International

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Edible bird's nest (EBN), the swiftlet's nest, has been consumed for centuries as a tonic or health food. The information upon the usage and benefits of the EBN are largely based on historical and observational reports since its discovery, until some were proven scientifically at recent times. Studies have been conducted on the major components of the EBN, i.e. glycoprotein, in attempts to elucidate the roles of the glycoprotein in terms of functionality toward health improvement. This article presents a review of the physicochemical and nutritional composition of EBN from different harvested locations and its functional properties. The conversion of EBN glycoproteins to glycopeptides via suitable hydrolysis methods to produce bioactive EBN glycopeptides was demonstrated, and it showed improvements in the functionality and nutritional values of EBN. Some applications of EBN glycopeptides into the new development of food and beverage products showed positive results, implicated to the bioavailability of the EBN glycopeptides. The combined information in this review will benefit the scientific communities, EBN manufacturers, and nutraceutical industries.

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In vitro and in vivo mechanism of immunomodulatory and antiviral activity of Edible Bird's Nest (EBN) against influenza A virus (IAV) infection

Abstract: The findings of this study support the antiviral activity of EBN against influenza virus and validate the traditional usage of this natural remedy by elucidation of toxicity and the molecular mechanism of action.

Cited by 65 publications

References 59 publications

Experimental validation and computational modeling of anti-influenza effects of quercetin-3-O-α-L-rhamnopyranoside from indigenous south African medicinal plant Rapanea melanophloeos

Experimental validation and computational modeling of anti-influenza effects of quercetin-3-O-α-L-rhamnopyranoside from indigenous south African medicinal plant Rapanea melanophloeos

Chinese Medicine in the Management of New and Emerging Infectious Diseases

Edible Bird’s Nest: Physicochemical Properties, Production, and Application of Bioactive Extracts and Glycopeptides

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