In vitro and in vivo activity of gallic acid and Toona sinensis leaf extracts against HL-60 human premyelocytic leukemia

Huang, Pei-Jane; Hseu, You‐Cheng; Lee, Meng-Shiou; Kumar, K. J. Senthil; Wu, Chi‐Rei; Hsu, Li‐Sung; Liao, Jiunn‐Wang; Cheng, I-Shiung; Kuo, Ya‐Ting; Huang, Shiying; Yang, Hsin‐Ling

doi:10.1016/j.fct.2012.06.046

Cited by 31 publications

(23 citation statements)

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“…These include both secreted molecules, such as the chemokine family of chemoattractant cytokines, and surface-expressed cell adhesion molecules, including the selectin and immunoglobulin families [35]. In our previous study, we reported that treatment with TS (25–75 μ g/mL) significantly as well as dose-dependently inhibited the VEGF expression in human premyelocytic leukemia HL-60 cells [16]. In this study we demonstrate that TS (25–100 μ g/mL) and GA (5 μ g/mL) significantly diminish the expression of VEGF, PDGF, and VCAM-1 in LPS-challenged SMCs, the pronounced inhibition was observed at a concentration of 75 μ g/mL of TS, whereas GA (5 μ g/mL) inhibits LPS-induced VEGF and PDGF expression, but not VCAM-1 expression in A7r5 cells.…”

Section: Discussionmentioning

confidence: 99%

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Toona sinensisInhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-κB Signaling Pathway

Yang

Huang

Liu

et al. 2014

Oxidative Medicine and Cellular Longevity

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Toona sinensis is one of the most popular vegetarian cuisines in Taiwan and it has been shown to possess antioxidant, antiangiogenic, and anticancer properties. In this study, we investigated the antiatherosclerotic potential of aqueous leaf extracts from Toona sinensis (TS; 25–100 μg/mL) and its major bioactive compound, gallic acid (GA; 5 μg/mL), in LPS-treated rat aortic smooth muscle (A7r5) cells. We found that pretreatment with noncytotoxic concentrations of TS and GA significantly inhibited inflammatory NO and PGE2 production by downregulating their precursors, iNOS and COX-2, respectively, in LPS-treated A7r5 cells. Furthermore, TS and GA inhibited LPS-induced intracellular ROS and their corresponding mediator, p47phox. Notably, TS and GA pretreatment significantly inhibited LPS-induced migration in transwell assays. Gelatin zymography and western blotting demonstrated that treatment with TS and GA suppressed the activity or expression of MMP-9, MMP-2, and t-PA. Additionally, TS and GA significantly inhibited LPS-induced VEGF, PDGF, and VCAM-1 expression. Further investigation revealed that the inhibition of iNOS/COX-2, MMPs, growth factors, and adhesion molecules was associated with the suppression of NF-κB activation and MAPK (ERK1/2, JNK1/2, and p38) phosphorylation. Thus, Toona sinensis may be useful for the prevention of atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

“…To identify these compounds, their spectral data (IR, NMR, and mass) were compared with analogous information reported in the literature [23, 24]. Gallic acid (GA), a natural phenolic component purified from TS extracts, was the main subject of this study, and it was produced with a yield of 10% [16]. …”

Section: Methodsmentioning

confidence: 99%

Toona sinensisInhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-κB Signaling Pathway

Yang

Huang

Liu

et al. 2014

Oxidative Medicine and Cellular Longevity

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“…Decreased levels of cyclin D1 and E, CDK 4 and hyperphosphorylated retinoblastoma protein were observed as well as increased levels of p27. Huang et al (2012) reported that Toona sinensis derived gallic acid caused G 1 arrest in HL-60 human promyelocytic cells. The PST was shown to contain gallic acid, proanthocyanidins as well as dihydroxycoumarins, and it is suspected that these compounds may be involved in the G 0 /G 1 phase block of the cell cycle observed in the Jurkat cell line.…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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Context Pelargonium sidoides DC (Geraniaceae) is an important medicinal plant indigenous to South Africa and Lesotho. Previous studies have shown that root extracts are rich in polyphenolic compounds with antibacterial, antiviral and immunomodulatory activities. Little is known regarding the anticancer properties of Pelargonium sidoides extracts. Objective This study evaluates the anti-proliferative effects of a Pelargonium sidoides radix mother tincture (PST). Materials and methods The PST was characterized by LC-MS/MS. Anti-proliferative activity was evaluated in the pre-screen panel of the National Cancer Institute (NCI-H460, MCF-7 and SF-268) and the Jurkat leukaemia cell line at concentrations of 0-150 mg/mL. The effect on cell growth was determined with sulphorhodamine B and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays after 72 h. The effect on cell cycle and apoptosis induction in Jurkat cells was determined by flow cytometry with propidium iodide and Annexin V: fluorescein isothiocyanate staining. Results Dihydroxycoumarin sulphates, gallic acid as well as gallocatechin dimers and trimers were characterized in PST by mass spectrometry. Moderate anti-proliferative effects with GI 50 values between 40 and 80 mg/mL were observed in the NCI-pre-screen panel. Strong activity observed with Jurkat cells with a GI 50 value of 6.2 mg/mL, significantly better than positive control 5-fluorouracil (GI 50 value of 9.7 mg/mL). The PST arrested Jurkat cells at the G 0 /G 1 phase of the cell cycle and increased the apoptotic cells from 9% to 21%, while the dead cells increased from 4% to 17%. Conclusion We present evidence that P. sidoides has cancer cell type-specific anti-proliferative effects and may be a source of novel anticancer molecules. ARTICLE HISTORY

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“…By using MTT assays in vitro, Chen et al (2011a) found that ethyl acetate extracts of T. sinensis (ACTSL) significantly inhibited the proliferation of leukemia K562 cell line with the IC 50 was 102.53 g/ml. Later in 2012, it is reported that water extracts of T. sinensis (TSL) could arrest leukemia HL-60 cells at G1-S transition phase and down-regulate VEGF (Huang et al, 2012). In 2016, research of Yang et al (2017) found that TSL (50 mg/kg) had notable antitumor effect against leukemia in WEHI-3 cells bearing mice.…”

Section: Anti-tumor Effectmentioning

confidence: 99%

Toona sinensis: a comprehensive review on its traditional usages, phytochemisty, pharmacology and toxicology

Peng

Liu

et al. 2019

Revista Brasileira de Farmacognosia

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a b s t r a c tToona sinensis (Juss.) M.Roem, Meliaceae, a deciduous plant native to eastern and southeastern Asia, is widely used in Traditional Chinese Medicine. This paper was aimed to summarize the current advances in traditional usage, phytochemistry, pharmacology and toxicology of T. sinensis. In this review, various types of data of T. sinensis are discussed in the corresponding parts of this paper, and perspectives for possible future studies of this plant are discussed. The main constituents of T. sinensis are terpenoids, phenylpropanoids and flavonoids, etc., and its pharmacological activities include anti-tumor effects, antioxidant activities, anti-diabetic effects and anti-inflammatory effects. Although a series of phytochemical and pharmacological researches of this plant have been conducted, the active constituents and action mechanism of these activities should be also further explored. Furthermore, the present review also indicates that T. sinensis has potentials to develop into drugs for treating various diseases with high efficacy and low toxicity, particularly in cancer, diabetes and inflammatory disorders. In conclusion, the paper provides a full-scale profile of the traditional usage, phytochemistry, pharmacology and toxicology of T. sinensis, and also provides potential therapeutic uses and drug development prospects of this plant.

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In vitro and in vivo activity of gallic acid and Toona sinensis leaf extracts against HL-60 human premyelocytic leukemia

Cited by 31 publications

References 50 publications

Toona sinensisInhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-κB Signaling Pathway

Toona sinensisInhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-κB Signaling Pathway

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Toona sinensis: a comprehensive review on its traditional usages, phytochemisty, pharmacology and toxicology

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In vitro and in vivo activity of gallic acid and Toona sinensis leaf extracts against HL-60 human premyelocytic leukemia

Cited by 31 publications

References 50 publications

Toona sinensisInhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-κB Signaling Pathway

Toona sinensisInhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-κB Signaling Pathway

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Toona sinensis: a comprehensive review on its traditional usages, phytochemisty, pharmacology and toxicology

Contact Info

Product

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Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells