In vitro and in vivo anti-inflammatory effects of theaflavin-3,3′-digallate on lipopolysaccharide-induced inflammation

Wu, Yanting; Jin, Fujun; Wang, Yiliang; Li, Feng; Wang, Lu; Wang, Qiaoli; Ren, Zhe

doi:10.1016/j.ejphar.2016.11.027

Cited by 69 publications

(51 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the Ligature group, the mRNA expression of IL‐6 , Gro/Cinc‐1 ( IL‐8 ), and Mmp‐9 was significantly increased compared with the Control group, but was significantly downregulated in the TF10 and TF100 groups. These results are in agreement with previous studies, which reported that TFs can inhibit IL‐6 expression by downregulating NF‐κB activation . In addition, TFs can attenuate the secretion of IL‐8 in oral epithelial cells after stimulation with LPS by inhibiting activation of the IkappaB kinase and activator protein‐1 pathways .…”

Section: Discussionsupporting

confidence: 93%

“…41 Moreover, we used qPCR to assess changes in the mRNA expression inhibit IL-6 expression by downregulating NF-κB activation. 20,21,42 In addition, TFs can attenuate the secretion of IL-8 in oral epithelial cells after stimulation with LPS by inhibiting activation of the IkappaB kinase and activator protein-1 pathways. 25 Opg, osteoprotegerin.…”

Section: Number Of Cells/bone Surface Length (Cells/mm)mentioning

confidence: 99%

“…TFs comprise a mixture of theaflavin (TF‐1), theaflavin‐3‐gallate (TF‐2a), theaflavin‐3′‐gallate (TF‐2b), and theaflavin‐3,3′‐digallate (TF‐3) . Similar to catechins, previous studies have shown that TFs have many positive effects on human health including antioxidant, antiinflammatory, and antitumor effects . Furthermore, compared with catechins, TF‐3 more effectively blocked lipopolysaccharide (LPS)‐induced nuclear factor‐kappa B (NF‐κB) activation by inhibiting IkappaB kinase activity in murine macrophages and more effectively inhibited osteoclast formation …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of theaflavins on tissue inflammation and bone resorption on experimental periodontitis in rats

Kuraji

Taya

et al. 2018

J of Periodontal Research

View full text Add to dashboard Cite

Background and ObjectiveTheaflavins (TFs), the major polyphenol in black tea, have the ability to reduce inflammation and bone resorption. The aim of this study was to evaluate the effects of TFs on experimental periodontitis in rats.Material and MethodsThirty rats were divided into five groups: Control (glycerol application without ligation), Ligature (glycerol application with ligation), TF1 (1 mg/mL TF application with ligation), TF10 (10 mg/mL TF application with ligation), and TF100 (100 mg/mL TF application with ligation). To induce experimental periodontitis, ligatures were placed around maxillary first molars bilaterally. After ligature placement, 100 μL glycerol or TFs were topically applied to the rats daily, and rats were euthanized 7 days after ligature placement. Micro‐computed tomography was used to measure bone resorption in the left side of the maxilla, and quantitative polymerase chain reaction was used to measure the expression of interleukin (IL)‐6, growth‐regulated gene product/cytokine‐induced neutrophil chemoattractant (Gro/Cinc‐1, rat equivalent of IL‐8), matrix metalloproteinase‐9 (Mmp‐9), receptor activator of nuclear factor‐kappa Β ligand (Rankl), osteoprotegerin (Opg), and the Rankl/Opg ratio in gingival tissue. With tissue from the right side of the maxilla, hematoxylin and eosin staining was used for histological analysis, immunohistochemical staining for leukocyte common antigen (CD45) was used to assess inflammation, and tartrate‐resistant acid phosphatase (TRAP) staining was used to observe the number of osteoclasts.ResultsThe TF10 and TF100 groups, but not the TF1 group, had significant inhibition of alveolar bone loss, reduction in inflammatory cell infiltration in the periodontium, and significantly reduced numbers of CD45‐positive cells and TRAP‐positive osteoclasts compared with the Ligature group. Correspondingly, the TF10 and TF100 groups had significantly downregulated gene expression of IL‐6, Gro/Cinc‐1(IL‐8), Mmp‐9, and Rankl, but not of Opg. Consequently, Rankl/Opg expression was significantly increased in the Ligation group but was attenuated in the TF10 and TF100 groups.ConclusionThe results of this study suggest that topical application of TFs may reduce inflammation and bone resorption in experimental periodontitis. Therefore, TFs have therapeutic potential in the treatment of periodontal disease.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Number Of Cells/bone Surface Length (Cells/mm)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of theaflavins on tissue inflammation and bone resorption on experimental periodontitis in rats

Kuraji

Taya

et al. 2018

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

“…Recently, it is reported to exhibit anticancer effects in various cancer types by inhibiting cell growth and inducing apoptosis as well as some antiinflammatory effects through downregulating proinflammatory cytokines. [24][25][26][27] Mechanically, TF-3 prevents tumorigenesis by decreasing MYC expression level. 28 Interestingly, TF-3 is identified as an inhibitor of NSD3/MYC interaction in our assay.…”

Section: Discussionmentioning

confidence: 99%

Development of a Time-Resolved Fluorescence Resonance Energy Transfer Ultrahigh-Throughput Screening Assay for Targeting the NSD3 and MYC Interaction

Xiong

González-Pecchi

Qui

et al. 2018

ASSAY and Drug Development Technologies

View full text Add to dashboard Cite

Epigenetic modulators play critical roles in reprogramming of cellular functions, emerging as a new class of promising therapeutic targets. Nuclear receptor binding SET domain protein 3 (NSD3) is a member of the lysine methyltransferase family. Interestingly, the short isoform of NSD3 without the methyltransferase fragment, NSD3S, exhibits oncogenic activity in a wide range of cancers. We recently showed that NSD3S interacts with MYC, a central regulator of tumorigenesis, suggesting a mechanism by which NSD3S regulates cell proliferation through engaging MYC. Thus, small molecule inhibitors of the NSD3S/MYC interaction will be valuable tools for understanding the function of NSD3 in tumorigenesis for potential cancer therapeutic discovery. Here we report the development of a cell lysate-based timeresolved fluorescence resonance energy transfer (TR-FRET) assay in an ultrahigh-throughput screening (uHTS) format to monitor the interaction of NSD3S with MYC. In our TR-FRET assay, anti-Flag-terbium and anti-glutathione S-transferase (GST)-d2, a paired fluorophores, were used to indirectly label Flag-tagged NSD3 and GST-MYC in HEK293T cell lysates. This TR-FRET assay is robust in a 1,536-well uHTS format, with signal-to-background >8 and a Z 0 factor >0.7. A pilot screening with the Spectrum library of 2,000 compounds identified several positive hits. One positive compound was confirmed to disrupt the NSD3/MYC interaction in an orthogonal protein-protein interaction assay. Thus, our optimized uHTS assay could be applied to future scaling up of a screening campaign to identify small molecule inhibitors targeting the NSD3/MYC interaction.

show abstract

“…Antiinflammatory agents may mitigate the effect of MeHg by reducing the amount of ROS formed. A natural compound derived from black tea, possess anti-inflammatory properties and it might be a potential therapeutic candidate for reducing inflammation [18]. Theaflavin-3,3'digigallate possess several active properties, one of which is the ability to fight ovarian cancer [19,20].…”

Section: Adipokines and Anti-inflammatory Agentsmentioning

confidence: 99%

Methyl Mercury, Adipokines, 3T3-L1 Cells and Diabetes

Lk¹

2016

ACT

View full text Add to dashboard Cite

Diabetes is a contributor to morbidity across the globe and is often associated with obesity and metabolic syndrome. In addition to genetic and lifestyle factors, environmental factors such as metals and persistent organic pollutants may increase the severity or lower the threshold of these conditions. Studies are showing an association between these contaminants and both insulin sensitivity and glucose transport. In cell culture, mercury and methyl mercury are toxic to adipocytes and impact the secretion of cytokines and adipokines. We propose a research model using contaminants like methyl mercury on adipocytes to enhance the existing knowledge on the mechanistic influence of adipokines and reactive oxygen species on 3T3-L1 cell functioning. With this enhanced signaling model, anti-inflammatory agents could be tested at the biochemical level and lead to studies in animal models. Prospective model studies on mixtures of contaminants can contribute to better understanding about the development or severity of diabetes.

show abstract

In vitro and in vivo anti-inflammatory effects of theaflavin-3,3′-digallate on lipopolysaccharide-induced inflammation

Cited by 69 publications

References 39 publications

Effects of theaflavins on tissue inflammation and bone resorption on experimental periodontitis in rats

Effects of theaflavins on tissue inflammation and bone resorption on experimental periodontitis in rats

Development of a Time-Resolved Fluorescence Resonance Energy Transfer Ultrahigh-Throughput Screening Assay for Targeting the NSD3 and MYC Interaction

Methyl Mercury, Adipokines, 3T3-L1 Cells and Diabetes

Contact Info

Product

Resources

About