In vitro and in vivo evaluation of the genotoxicity of Eriobotrya japonica leaf extract

Jeong, Myung-Hwan; Seong, Nak-Won; Lee, Jong-Yun; Kim, Yong-Jae; Shin, Na‐Rae; Kim, Jong-Choon

doi:10.1016/j.yrtph.2018.09.029

Cited by 9 publications

(1 citation statement)

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“…Consequently, in the present studies, no or a low clastogenic or aneugenic effect was obtained in the treatment regimen in bone marrow cells, and a low genotoxicity in barley was obtained after application of hydrosol to R. damascena. Our findings with the plant test system in vivo and animal test system in vivo reciprocate earlier investigations reporting a lack of chromosome damage [62][63][64] in various chemopreventive herbal extracts and phytochemical studies. Since cytogenetic results for geraniol, which together with citronellol are the main constituents of the R. alba and R. damascena hydrosols, are lacking in the literature, our results are expected and logical.…”

Section: Discussionsupporting

confidence: 89%

Study on Cytotoxic and Genotoxic Potential of Bulgarian Rosa damascena Mill. and Rosa alba L. Hydrosols—In Vivo and In Vitro

Gerasimova

Jovtchev

Gateva

et al. 2022

Life

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The Rosa alba L. and Rosa damascena Mill. growing in Bulgaria are known for their extremely fine essential oil and valuable hydrosols. Irrespectively of its wide use in human life, little research exists on the cytotoxic and genotoxic activity of the hydrosols. This set our goal to conduct cytogenetic analyses to study these effects. A complex of classical cytogenetic methods was applied in three types of experimental test systems—higher plant in vivo, ICR mice in vivo, and human lymphocytes in vitro. Mitotic index, PCE/(PCE + NCE) ratio, and nuclear division index were used as endpoints for cytotoxicity and for genotoxicity—induction of chromosome aberrations and micronuclei. Rose hydrosol treatments range in concentrations from 6% to 20%. It was obtained that both hydrosols did not show considerable cytotoxic and genotoxic effects. These effects depend on the type of the tested rose hydrosols, the concentrations applied in the experiments, and the sensitivity and specificity of the test systems used. Human lymphocytes in vitro were the most sensitive to hydrosols, followed by higher plant and animal cells. Chromosomal aberrations and micronucleus assays suggested that R. damascena and R. alba hydrosols at applied concentrations possess low genotoxic risk. Due to the overall low values in terms of cytotoxic and/or genotoxic effects in all test systems, hydrosols are promising for further use in various areas of human life.

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Section: Discussionsupporting

confidence: 89%