In vitro and in vivo comparative colonization of Staphylococcus aureus and Staphylococcus epidermidis on orthopaedic implant materials

Barth, Elin; Myrvik, Quentin N.; Wagner, William D.; Gristina, Anthony G.

doi:10.1016/0142-9612(89)90073-2

Cited by 219 publications

(110 citation statements)

References 17 publications

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“…The possible explanation for such subclinical infection status may be that microorganisms identified are most likely to be opportunists and lowly virulent strains. Whether infection recurs or not will eventually rely upon interaction between microorganisms, the implant and the host [36][37][38]. Weakening of the body defenses at the implant surface-tissue interface will facilitate the establishment of an infection around the implant.…”

Section: Discussionmentioning

confidence: 99%

Bacteria detected after instrumentation surgery for pyogenic vertebral osteomyelitis in a canine model

et al. 2013

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Objective This study was designed to identify the presence, type and origin of bacteria adjacent to the metal implant in the infected region in a canine model of pyogenic vertebral osteomyelitis treated with single-stage anterior autogenous bone grafting and instrumentation. Methods Dogs with pyogenic spondylodiscitis underwent one-stage debridement, autogenous bone grafting and titanium plate instrumentation and perioperative antibiotic therapy. The implants and adjacent vertebral bones were removed surgically at various postoperative time points (4, 8, 12 and 24 weeks) for bacteria detection. Bacteria were detected from retrieved spinal implants as well as surrounding bones by culture and/or pyrosequencing methods in 17 (85 %) of all 20 animals. The positive rate for bacteria presence was 45 % by culture and 80 % by pyrosequencing method. Results Radiological or macroscopic examination showed no signs for infection recurrence in any animal regardless of bacteria presence at the surgical site. However, organism identical with the causative bacterium for spinal infection was found in only two of nine culturepositive animals. Conclusion Within the confines of the study, the use of metallic implants in an infected area did not lead to a clinically relevant infection although bacteria may exist at the surgical site. The use of metallic implants in an infected area of the spine is safe. The metallic implants may not be the ''culprit'' for the persistence or recurrence of infection.

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Section: Discussionmentioning

confidence: 99%

Bacteria detected after instrumentation surgery for pyogenic vertebral osteomyelitis in a canine model

et al. 2013

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“…Also, studies have shown that, in vitro, Staphylococcus aureus adhesion was significantly reduced on PLLA compared to metal whereas the inverse has been observed for Staphylococcus epidermis (Barth et al, 1989). Thus, it can then be summarized that the poly(α-hydroxyacid) compositions do not affect significantly bacterial adhesion and infection.…”

Section: Eglin and M Alinimentioning

confidence: 99%

Degradable polymeric materials for osteosynthesis: Tutorial

Eglin

Alini²

2008

eCM

111

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This report summarizes the state of the art and recent developments and advances in the use of degradable polymers devices for osteosynthesis. The current generation of biodegradable polymeric implants for bone repair utilising designs copied from metal implants, originates from the concept that devices should be supportive and as "inert" substitute to bone tissue. Today degradable polymeric devices for osteosynthesis are successful in low or mild load bearing applications. However, the lack of carefully controlled randomized prospective trials that document their efficacy in treating a particular fracture pattern is still an issue. Then, the choice between degradable and non-degradable devices must be carefully weighed and depends on many factors such as the patient age and condition, the type of fracture, the risk of infection, etc. The improvement of the biodegradable devices mechanical properties and their degradation behaviour will have to be achieved to broaden their use. The next generation of biodegradable implants will probably see the implementation of the recent gained knowledge in cellmaterial interactions and cells therapy, with a better control of the spatial and temporal interfaces between the material and the surrounding bone tissue.

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“…Along with changes in the chemical composition of a substratum surface after conditioning film formation (Barth, 1989;Hogt et al, 1985;Oga et al, 1988), physicochemical properties of the surface such as its hydrophobicity, roughness, charge and elasticity may alter (Bakker et al, 2003b). Although many studies have shown relationships between substratum hydrophobicity, charge or roughness with bacterial adhesion, it is still hard to understand how the multiple changes brought about by the adsorption of a conditioning film affect bacterial adhesion.…”

Section: Introductionmentioning

confidence: 99%

Multiple linear regression analysis of bacterial deposition to polyurethane coatings after conditioning film formation in the marine environment

et al. 2004

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Many studies have shown relationships of substratum hydrophobicity, charge or roughness with bacterial adhesion, although bacterial adhesion is governed by interplay of different physico-chemical properties and multiple regression analysis would be more suitable to reveal mechanisms of bacterial adhesion. The formation of a conditioning film of organic compounds adsorbed from seawater affects the properties of substratum surfaces prior to bacterial adhesion, which is a complicating factor in studying the mechanism of bacterial adhesion. In this paper, the impact of conditioning films adsorbed from natural seawater to four polyurethane coatings with different hydrophobicity, elasticity and roughness was studied for three different marine bacterial strains in a multiple linear regression analysis. The water contact angle on hydrophobic coatings decreased on average by 8 degrees and increased on average by the same amount on hydrophilic coatings. These changes were accompanied by increased concentrations of oxygen and nitrogen on the surface as determined by X-ray photoelectron spectroscopy, indicative of adsorption of proteinaceous material. Furthermore, the mean surface roughness increased on average by 4 nm after conditioning film formation. Multiple linear regression analysis revealed that changes in deposition due to conditioning film formation of Marinobacter hydrocarbonoclasticus, Psychrobacter sp. SW5H and Halomonas pacifica in a stagnation-point flow chamber could be explained in a model comprising hydrophobicity and the prevalence of nitrogen-rich components on the surface for the most hydrophobic strain. For the two more hydrophilic strains, deposition was governed by a combination of surface roughness and hydrophobicity. Elasticity was not a factor in bacterial adhesion to conditioning films.

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In vitro and in vivo comparative colonization of Staphylococcus aureus and Staphylococcus epidermidis on orthopaedic implant materials

Cited by 219 publications

References 17 publications

Bacteria detected after instrumentation surgery for pyogenic vertebral osteomyelitis in a canine model

Bacteria detected after instrumentation surgery for pyogenic vertebral osteomyelitis in a canine model

Degradable polymeric materials for osteosynthesis: Tutorial

Multiple linear regression analysis of bacterial deposition to polyurethane coatings after conditioning film formation in the marine environment

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