In vitro and in vivo anti-inflammatory activity of Phyllanthus acidus methanolic extract

Hossen, Muhammad Jahangir; Jeon, Sung Ho; Kim, Seung Cheol; Kim, Ji Hye; Jeong, Deok; Sung, Nak Yoon; Yang, Soo Jin; Baek, Ki Hyun; Kim, Jun Ho; Yoon, Deok Hyo; Song, Won O.; Yoon, Kee Dong; Cho, Sang-Ho; Lee, Sukchan; Kim, Jong Hoon; Cho, Jae Youl

doi:10.1016/j.jep.2015.03.043

Cited by 57 publications

(26 citation statements)

References 53 publications

(59 reference statements)

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“…RAW264.7 and HEK293 cells were cultured in RPMI 1640 and Dulbecco's modified Eagle's medium, respectively, supplemented with 10% heat-inactivated fetal bovine serum and 1% antibiotics (penicillin and streptomycin) at 37°C under 5% CO 2 [9].…”

Section: Methodsmentioning

confidence: 99%

Anticancer effect of joboksansam, Korean wild ginseng germinated from bird feces

Park

Kang²,

Park

et al. 2016

Journal of Ginseng Research

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BackgroundJoboksansam, Korean bird wild ginseng, is an artificially cultivated wild ginseng germinated from bird feces. Although numerous pharmacologic activities of wild ginsengs have been reported, the beneficial effect of joboksansam in cancer has not been elucidated. In this study, we investigated the in vivo and in vitro anticancer activities of joboksansam powder.MethodsTo evaluate the in vivo anticancer activity of joboksansam, we established a xenograft mouse model bearing RMA cell-derived cancer. Direct cytotoxicity induced by joboksansam powder was also investigated in vitro using (3-4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) assay. The inhibitory activity of this powder on the activation of cell survival signaling involving Akt and Src was examined with immunoblot analysis.ResultsJoboksansam powder displayed strong inhibitory activity against the increased tumor size, increased weight of total body and cancer tissues, and mortality of tumor-bearing mice. Joboksansam powder also suppressed the activation of survival regulatory enzymes Akt and Src, as assessed by phosphorylation levels in the immunoblot analysis of tumor tissues. Interestingly, the viability of RMA cells in vitro was directly decreased by joboksansam treatment.ConclusionOverall, our results strongly suggest that joboksansam powder has the potential to protect against cancer generation by direct cytotoxic effects on cancer cells resulting from suppression of cell survival signaling.

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Section: Methodsmentioning

confidence: 99%

Anticancer effect of joboksansam, Korean wild ginseng germinated from bird feces

Park

Kang²,

Park

et al. 2016

Journal of Ginseng Research

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“…Tissue-associated macrophages play a critical role in the loss of organ function by releasing toxic and inflammatory molecules such as NO, ROS, PGE 2 , hydrolytic enzymes, and proinflammatory cytokines Hossen et al, 2015b;Kim et al, 2014b). The release of these molecules can cause gastritis, arthritis, hepatitis, and colitis (Yang et al, 2014a;Yang et al, 2014b;Yi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…CRL-1573), were purchased from ATCC (Rockville, MD, USA). Luciferase constructs containing binding sites for NF-κB and PDK1 were used as reported previously (Hossen et al, 2015b) (Byeon et al, 2013). All other chemicals were purchased from Sigma.…”

Section: Methodsmentioning

confidence: 99%

“…The stomach was then excised and gently rinsed under running tap water. After opening the stomach along the greater curvature and spreading it out on a board, the area (mm 2 ) of each mucosal erosive lesion was measured using custom ImageJ-based software, as reported previously (Hossen et al, 2015b).…”

Section: Etoh/hcl-induced Gastritis In Vivomentioning

confidence: 99%

“…In the innate defense system, both acute and chronic inflammatory responses play critical roles in maintaining immune homeostasis . Most studies on inflammation have focused on the systemic, cellular, and molecular mechanisms of inflammatory responses (Hossen et al, 2015b). However, hyperactive and prolonged inflammation is known to cause various chronic diseases including cancer, diabetes, atherosclerosis, autoimmune disorders, arthritis, and vascular diseases (Ham and Moon, 2013;Jung et al, 2014;Kim et al, 2014a;Lyman et al, 2014;Momi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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PDK1 in NF-κB signaling is a target of Xanthium strumarium methanolic extract-mediated anti-inflammatory activities

Hossen

Cho

Kim

2016

Journal of Ethnopharmacology

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Phytochemical Profiling, Antioxidants, Antimicrobial and Anti‐Proliferative Effect of Senna hirsuta against PC‐3 Human Prostate Cancer Cells

et al. 2022

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The phytochemical profile, antioxidant, antibacterial, and cytotoxicity of Senna hirsuta (MÀ Sh) leaf extracts were investigated in human prostate cancer PC-3 cell lines. Antioxidant bioactive components like phenolics, tannins, and flavonoids were measured at 15.36 � 0.19, 2.97 � 0.11, and 19.77 � 0.51, respectively. An intense dose-dependent radical scavenging activity was observed for MÀ Sh with 83 % in 500 μg/mL for DPPH. MÀ Sh boosted ABTS radical inhibition from 35 % at 100 μg/ml to 70 % at 500 μg/ml, whereas hydroxyl radical scavenging was 96 % at 500 μg/ml, significantly greater than butylated hydroxytoluene (91.91 %). The maximum NO scavenging activity of MÀ Sh was 83 %, and the lipid peroxidation inhibition was 87 % at 500 μg/ml. The MÀ Sh exhibited antibacterial efficacy against Escherichia coli (25 mm), Bacillus subtilis (24 mm), and Staphylococcus aureus (21 mm). The MTT assay was used to study anti-proliferative activity, which inhibited PC-3 cell growth with an IC50 of 257.13 μg/mL over 24 hours. These results demonstrate that MÀ Sh inhibits PC-3 cell proliferation by shedding from the surface and assuming a spherical shape. Additionally, the morphology of PC-3 cells showed shrinkage, blebbing membrane condensation, and nuclear chromatin margination, indicating that MÀ Sh was cytotoxic to PC-3 Cells.

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In vitro and in vivo anti-inflammatory activity of Phyllanthus acidus methanolic extract

Cited by 57 publications

References 53 publications

Anticancer effect of joboksansam, Korean wild ginseng germinated from bird feces

Anticancer effect of joboksansam, Korean wild ginseng germinated from bird feces

PDK1 in NF-κB signaling is a target of Xanthium strumarium methanolic extract-mediated anti-inflammatory activities

Phytochemical Profiling, Antioxidants, Antimicrobial and Anti‐Proliferative Effect of Senna hirsuta against PC‐3 Human Prostate Cancer Cells

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