In vitro and in vivo assessment of phage therapy against Staphylococcus aureus causing bovine mastitis

Tchamba, Cyrille Ngassam; Duprez, Jean-Noël; Fergestad, M.; Visscher, Alex De; L’Abée-Lund, Trine M.; Vliegher, Sarne De; Wasteson, Yngvild; Touzain, Fabrice; Blanchard, Yannick; Lavigne, Rob; Chanishvili, Nina; Cassart, Dominique; Mainil, Jacques; Thiry, Damien

doi:10.1016/j.jgar.2020.06.020

Cited by 38 publications

(28 citation statements)

References 26 publications

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“…Previous studies demonstrated in vitro e ciency and speci city S. aureus phages against S. aureus isolates from bovine mastitis [22,24,28,[56][57][58][59]. In our research, both phages also demonstrated lytic e ciency mainly against S. aureus and CPS of isolates.…”

Section: Discussionsupporting

confidence: 68%

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Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

Barasuol

Cargnelutti

Sangioni

et al. 2021

Preprint

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Bovine mastitis is an important disease of dairy cows, and Staphylococcus aureus is the etiologic agent most prevalent among the microorganisms. Mastitis caused by S. aureus present low cure rate with antimicrobials treatment and low vaccines efficacy. Bacteriophages or phages have been considered as an alternative for treating this disease. This study, we isolated and characterized two new S. aureus phages, namely B_UFSM4 and B_UFSM5, from bovine milk of cows with mastitis. The adsorptions rates were 10–20 min for B_UFSM4 and 20–30 min for B_UFSM5. Phages activities were relatively stable at pH 3–11; however, at temperatures of 50 °C-60ºC-70ºC/60 min, the phages were completely inactivated. These viruses presented infectivity in various bacteria isolated from bovine mastitis, where the lytic activity of phages B_UFSM4 and B_UFSM5 were 34.2%(13/38) and 42.1%(16/38), respectively, including isolates from S. aureus, Pseudomonas aeruginosa, Staphylococcus sciuri, and Rothia terrae. The complete genomes of B_UFSM4 and B_UFSM5 have 41.396 bp and 41.829 bp, with GC-content 33.97% and 33.98%, respectively. Both phages comprise 61 putative ORFs. The viruses have double stranded DNA and linear architecture. Phylogenic similarity was observed by proteome with Staphylococcus prophage phiPV83 (45,536 nt), Staphylococcus phage CN125 (44,492 nt) and Staphylococcus phage JS01 (43,458 nt). Based on the morphology, the phages belong to Siphoviridae family, presenting icosahedral head with a long tail, Caudovirales order and Biseptimavirus genus. Thus, two S. aureus phages (B_UFSM4 and B_UFSM5) were isolated and characterized, and these phages can be used as therapeutic or prophylactic candidates against S. aureus infections in cattle mastitis.

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Section: Discussionsupporting

confidence: 68%

“…They may be important tools in controlling antimicrobial resistance and multiresistant bacteria [19]. Lytic phages of S. aureus have been isolated from bovine mastitis, and their e ciency and speci city are described [20][21][22]; however, some do not present lytic e ciency [23,24] and few demonstrate a wide host range [25].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

Barasuol

Cargnelutti

Sangioni

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The results are promising, showing 50% survival of larvae in G. mellonella within four days of in vivo treatment with the three phages. In the mouse model, incomplete recovery was obtained after 48 hours post-infection of a single phage [ 53 ]. The diminished effect may be explained by the possible interaction of the phages with proteins and lipids present in milk and by the use of a single phage [ 54 ].…”

Section: Phage Therapy In the Veterinary Fieldmentioning

confidence: 99%

“…The diminished effect may be explained by the possible interaction of the phages with proteins and lipids present in milk and by the use of a single phage [ 54 ]. However, this study demonstrates the potential usefulness of phage therapy as a treatment for mastitis caused by S. aureus [ 53 ]. Recently, more studies on phage therapy for mastitis in cattle have been developed with positive, significant results using phage cocktails in mouse models.…”

Section: Phage Therapy In the Veterinary Fieldmentioning

confidence: 99%

Phage Therapy in Livestock and Companion Animals

Ferriol-González

Domingo‐Calap

2021

Antibiotics

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The irrational use of antibiotics has led to a high emergence of multi-drug resistant (MDR) bacteria. The traditional overuse of antibiotics in the animal feed industry plays a crucial role in the emergence of these pathogens that pose both economic and health problems. In addition, antibiotics have also recently experienced an increase to treat companion animal infections, promoting the emergence of MDR bacteria in pets, which can reach humans. Phages have been proposed as an alternative for antibiotics for the treatment of livestock and companion animal infections due to their multiple advantages as adaptative drugs, such as their ability to evolve, to multiply at the site of infections, and their high specificity. Moreover, phage-derived enzymes may also be an interesting approach. However, the lack of regulation for this type of pharmaceutical hinders its potential commercialization. In this review, we summarize the main recent studies on phage therapy in livestock and companion animals, providing an insight into current advances in this area and the future of treatments for bacterial infections.

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“…Additionally, E.coli mastitis incurs subclinical phenotypes, including an increase in somatic cell count (SCC) in milk (Abdi et al, 2021) and the production of proinflammatory cytokines in blood (Cobirka et al, 2020). Currently, several measures to control mastitis have been taken, such as antibiotic therapy (Mcdougall et al, 2019), antimicrobial peptides (Gurao et al, 2017), bacteriophage therapy (Ngassam-Tchamba et al, 2020), probiotics (Pellegrino et al, 2018), and nanoparticle-based therapy (Orellano et al, 2019). Among them, because of their remarkable effectiveness, antibiotics are the most common method (Gomes and Henriques, 2016).…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage Cocktails Protect Dairy Cows Against Mastitis Caused By Drug Resistant Escherichia coli Infection

Guo

Gao

Xue

et al. 2021

Front. Cell. Infect. Microbiol.

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Mastitis caused by Escherichia coli (E. coli) remains a threat to dairy animals and impacts animal welfare and causes great economic loss. Furthermore, antibiotic resistance and the lagged development of novel antibacterial drugs greatly challenge the livestock industry. Phage therapy has regained attention. In this study, three lytic phages, termed vB_EcoM_SYGD1 (SYGD1), vB_EcoP_SYGE1 (SYGE1), and vB_EcoM_SYGMH1 (SYGMH1), were isolated from sewage of dairy farm. The three phages showed a broad host range and high bacteriolytic efficiency against E. coli from different sources. Genome sequence and transmission electron microscope analysis revealed that SYGD1 and SYGMH1 belong to the Myoviridae, and SYGE1 belong to the Autographiviridae of the order Caudovirales. All three phages remained stable under a wide range of temperatures or pH and were almost unaffected in chloroform. Specially, a mastitis infected cow model, which challenged by a drug resistant E. coli, was used to evaluate the efficacy of phages. The results showed that the cocktails consists of three phages significantly reduced the number of bacteria, somatic cells, and inflammatory factors, alleviated the symptoms of mastitis in cattle, and achieved the same effect as antibiotic treatment. Overall, our study demonstrated that phage cocktail may be a promising alternative therapy against mastitis caused by drug resistant E. coli.

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In vitro and in vivo assessment of phage therapy against Staphylococcus aureus causing bovine mastitis

Cited by 38 publications

References 26 publications

Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

Phage Therapy in Livestock and Companion Animals

Bacteriophage Cocktails Protect Dairy Cows Against Mastitis Caused By Drug Resistant Escherichia coli Infection

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