In vitro and in vivo Assessment of Anti-Leishmanial Efficacy of Leaf, Fruit, and Fractions of Juniperus excelsa Against Axenic Amastigotes of Leishmania major and Topical Formulation in BALB/c Mice

Mirzavand, Somayeh; Hatam, Gholamreza; Moein, Mahmoodreza; Zarshenas, Mohammad M.

doi:10.5812/ircmj.87754

Cited by 4 publications

(3 citation statements)

References 38 publications

(41 reference statements)

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“…For example, in an in vivo study, the anti‐leishmaniasis effect of barberry was proven 24 . Furthermore, the anti‐leishmanial activity of the extract of fruits and leaves of Juniperus excelsa M. Bieb has been proven in both in vitro and in vivo studies 25 26 .…”

Section: Resultsmentioning

confidence: 99%

“…24 Furthermore, the anti-leishmanial activity of the extract of fruits and leaves of Juniperus excelsa M. Bieb has been proven in both in vitro and in vivo studies. 25 In another in vitro study, the antileishmaniasis effect of the extract of leaf of Aloe vera was revealed. 26 In addition, one clinical trial has shown that the extract of Nigella sativa L. mixed with honey accelerates the healing process of CL lesions.…”

Section: Manipulationmentioning

confidence: 98%

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Overview of herbal and traditional remedies in the treatment of cutaneous leishmaniasis based onTraditional Persian Medicine

Parvizi

Zare

Handjani

et al. 2020

Dermatologic Therapy

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This study aims to describe the herbal and traditional remedies in the treatment of cutaneous leishmaniasis (CL) with an overview on related available evidence in modern medicine. This study is a review that focuses on the most important Traditional Persian Medicine (TPM) sources including Avicenna's Canon of Medicine, Jorjani's Zakhīra‐yi Khārazmshāhī, and Aazam‐Khan's Eksir‐e‐Aazam, as well as pertinent information from Embase, PubMed, Scopus, Scientific Information Database, and Google Scholar by using the keywords salak, rīsh‐e‐balkhi, cutaneous leishmaniasis, and leishmaniasis for selected remedies. Several oral and topical herbal remedies, such as Vitis vinifera L. (Unripe grapes), Berberis vulgaris L., Rheum ribes L., Santalum album L., Cinnamomum camphora (L.) J.Presl (Camphor), Brassica nigra (L.) K. Koch, Crocus sativus L., Juniperus excelsa M. Bieb, honey, and Alum root, were mentioned in TPM resources for the treatment of CL. Furthermore, cauterization, cupping, and leech therapy were considered for this purpose. In this review, some evidence‐based studies will also be presented that have demonstrated the therapeutic properties of some of these products. In conclusion, the sages of TPM have recommended several systemic or topical medications, in addition to physical procedures, for treatment of CL, all of which could be a base for conducting further research on its efficacy.

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Section: Resultsmentioning

confidence: 99%

Section: Manipulationmentioning

confidence: 98%

Overview of herbal and traditional remedies in the treatment of cutaneous leishmaniasis based onTraditional Persian Medicine

Parvizi

Zare

Handjani

et al. 2020

Dermatologic Therapy

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“…The concentration of methanolic extract for antileishmanial assay in the cream formulation was considered to be 10% (W/W). The placebo cream was also prepared in the same manner (Mirzavand et al, 2019).…”

Section: Extraction Fractionation and General Proceduresmentioning

confidence: 99%

In vitro and in vivo antileishmanial activity of the hanging sedge flavonoids based on bio‐ guided fractionation assay

et al. 2023

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As a major public health issue, cutaneous leishmaniasis (CL) has a number of complications, including drug resistance and poor response to conventional treatments. Over the last decade, research on natural sources for finding new antileishmanial agents has been a critical part of tropical disease research. Natural products also should be regarded as one of the most valuable applications for CL infection drug development. In this study, we assessed the in vitro and in vivo antileishmanial potential of Carex pendula Huds. (hanging sedge) methanolic extract and its fractions against Leishmania major produced cutaneous infection. Although the methanolic extract and its fractions exhibited suitable activity, the ethyl acetate fraction showed the best activity (with the half maximal inhibitory concentration IC50 = 1.627 ± 0.211 mg/mL). The toxicity and selectivity indices (SI) of all samples were determined in murine peritoneal macrophage cells (J774A.1) using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) test. The flavonoid components of the ethyl acetate fraction were identified using liquid chromatography electrospray ionization mass spectrometry (LC‐ESI MS/MS). Nine chemical compounds were identified in this fraction, including three flavonols, four flavanonols, and two flavan derivatives. L. major‐infected mice were used as an in vivo model because the methanolic extract was effective against L. major promastigotes in the mammalian cell line J774A.1 with SI = 2.514 (tail lesion size model). In silico analysis of identified compounds also revealed a favorable interaction between compounds 2–5 and L. major protein targets (3UIB, 4JZX, 4JZB, 5L4N, and 5L42). According to the findings of this study, the ethyl acetate fraction (as flavonoid fraction) exhibited considerable in vitro antileishmanial activity.

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Apigenin effect against Leishmania tropica amastigotes in vitro

Naddaf

Haddad

2020

J Parasit Dis

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In vitro and in vivo Assessment of Anti-Leishmanial Efficacy of Leaf, Fruit, and Fractions of Juniperus excelsa Against Axenic Amastigotes of Leishmania major and Topical Formulation in BALB/c Mice

Cited by 4 publications

References 38 publications

Overview of herbal and traditional remedies in the treatment of cutaneous leishmaniasis based onTraditional Persian Medicine

Overview of herbal and traditional remedies in the treatment of cutaneous leishmaniasis based onTraditional Persian Medicine

In vitro and in vivo antileishmanial activity of the hanging sedge flavonoids based on bio‐ guided fractionation assay

Apigenin effect against Leishmania tropica amastigotes in vitro

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