In Vitro and In Vivo Relevance of Infectious Laryngotracheitis Virus gJ Proteins That Are Expressed from Spliced and Nonspliced mRNAs

Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update

“…Some viruses with deleted genes may retain their ability to induce immune response while not producing clinical signs or latency. ILT viruses with deletions of the genes gC (Pavlova et al, 2010), gG (Devlin et al, 2006), gJ (Fuchs et al, 2005), TK (Han et al, 2002), UL0 (Veits et al, 2003), and UL 47 (Helferich et al, 2007) showed attenuation and may be used for vaccine production. A gG-deficient ILTV strain administered to 3-week-old SPF chickens via drinking water and eye drop induced adequate immunity against challenge with a wild strain and may potentially be used for large-scale vaccination; however, further studies are needed before it is applied to commercial poultry flocks (Devlin et al, 2008).…”

Molecular biology of avian infectious laryngotracheitis virus

“…Deletion of gJ or the ILTV-specific UL0 gene rendered ILTV almost completely apathogenic for chickens (Fig. 1A) [26,92]. In contrast, dUTPase negative ILTV was shown to retain a considerable virulence after intratracheal application of high virus doses [25].…”

“…for infectious bovine rhinotracheitis, or Newcastle disease of chickens [18,59]. In particular, the ILTV gJ-and gC-specific MAb might be suitable for this type of assay, since naturally or experimentally infected chickens produce antibodies against these two glycoproteins, which are detectable by indirect immunofluorescence tests on cells expressing the cloned genes [26,90]. It was further shown that bacterial fusion proteins expressed from the cloned gJ (gp60) or gE genes of ILTV can be used as antigens for detection of ILTV-specific serum antibodies in direct ELISA tests [13].…”

“…The pictures show a protected chicken (C) and a control animal suffering from severe dyspnea (D) at day 4 after challenge. For more details see reference [26]. (A colour version of this figure is available at www.edpsciences.org/vetres.)…”

“…multiple protein forms with apparent masses of 85, 115, 160 and 200 kDa. Two different primary translation products are formed from non-spliced and spliced mRNA, modified by N-and O-linked glycosylation, and presumably further processed by proteolytic cleavage [26].…”