In vitro and in vivo evaluation of mutagenicity of fucoxanthin (FX) and its metabolite fucoxanthinol (FXOH)

Abstract

fucoxanthin (FX), was evaluated by in vitro Ames test, and of FX by in vivo micronucleus test. In in vitro Ames test, bacterial reverse mutation was examined by using Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537, and Escherichia coli WP2uvr -vation by S9 mix in the preincubation method, and mutagenicity of FXOH was found to be negative in all cases. In in vivo micronucleus test, mice were orally administered with FX at doses of 500, 1,000 and 2,000 mg/kg, and the bone marrow cells were taken 24 hr after the administration to observe the incidence of micronucleus cells, and mutagenicity of FX was found to be negative at all doses. Based on the data of the present study it can be presumed that orally administered FX is a safe compound in terms of mutagenicity under the experimental conditions employed here.

Anticancer effects of seaweed compounds fucoxanthin and phloroglucinol, alone and in combination with 5-fluorouracil in colon cells

“…Beppu and colleagues, observed the incidence of micronucleus and mutagenicity of fucoxanthin in bone marrow cells of mice that were orally administered for 24h. The results for micronucleus and mutagenicity were found to be negative (Beppu et al, 2009). …”

Fucoxanthin, a Marine Carotenoid Present in Brown Seaweeds and Diatoms: Metabolism and Bioactivities Relevant to Human Health

“…Subsequently, Beppu et al . [56] indicated that fucoxanthin had no genotoxic/mutagenic effect on the bone marrow cells of mice. Iio et al .…”

“…In the 13-week oral dose study, the no-observed-adverse-effect level of fucoxanthin was 200 mg/kg body weight under the tested subchronic dose condition. These studies suggested that fucoxanthin was a safe compound and did not exhibit toxicity and mutagenicity under these experimental conditions [3,34,56]. …”

Nutraceutical Effects of Fucoxanthin for Obesity and Diabetes Therapy: A Review

“…Fucoxanthin toxicity has arisen as a concern from results of mice experiments 37,38 . Fucoxanthin was orally administered at doses of 500 and 1,000 mg/kg to male and female ICR mice for 30 days.…”