In vitro and in vivo performance of a propolis-coated polyurethane wound dressing with high porosity and antibacterial efficacy

Khodabakhshi, Darioush; Eskandarinia, Asghar; Kefayat, Amirhosein; Rafienia, Mohammad; Navid, Sepehr; Karbasi, Saeed; Moshtaghian, Jamal

doi:10.1016/j.colsurfb.2019.03.010

Cited by 95 publications

(45 citation statements)

References 48 publications

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“…Ethanolic extract added into the minced carp meat was efficient against the spoilage of microorganisms, including total plate counts, psychrotrophic, and lactic acid bacteria (Payandan et al, ). Antimicrobial activity of propolis against microorganisms in vivo and in vitro has been reported by Seibert et al () and Khodabakhshi et al ().…”

Section: Resultsmentioning

confidence: 80%

“…Duman and Özpolat (2015) reported a microbiological shelf life extension of 6, 9, and 15 days by addition of water extracts of propolis at doses of 0.1, 0.3, and 0.6%, respectively, for vacuum-packed fresh shibuta fillets at 2 C. Ethanolic extract added into the minced carp meat was efficient against the spoilage of microorganisms, including total plate counts, psychrotrophic, and lactic acid bacteria (Payandan et al, 2017). Antimicrobial activity of propolis against microorganisms in vivo and in vitro has been reported by Seibert et al (2019) and Khodabakhshi et al (2019).…”

Section: Microbiological Analysismentioning

confidence: 95%

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The impacts of water and ethanolic extracts of propolis on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans during chilly storage

Yazgan

Burgut

Durmuş

et al. 2020

Journal of Food Safety

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The impacts of water and ethanolic extracts of propolis at a dose of 0.4 or 0.8% on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans DSM 17886 during storage at 3 ± 1°C for 15 days were investigated. All fish groups were inoculated with M. psychrotolerans (108 cfu/ml) at a rate of 1%. Sensory, colorimetric, chemical analysis (total volatile basic nitrogen, thiobarbituric acid, peroxide values, and free fatty acids), pH value, and microbiological analysis (viable mesophilic and psychrophilic bacteria, coliform, and lactic acid bacteria count) were carried out. An enhance in L* values was found in the group treated with 0.8% ethanolic extracts of propolis on the seventh day of the storage. Application of propolis extract on fish fillets significantly inhibited bacterial growth during storage and extended shelf life of sardine for 4 and 6 days by the use of water extract and for 8 days by the use of ethanolic extract at doses of 0.4 and 0.8%, respectively. The result of the study revealed that application of propolis extracts, mainly ethanolic propolis extracts on sardine fillets resulted in lower lipid oxidation and bacterial growth, therefore, could be natural food additive for preservation of fish fillets.

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Section: Resultsmentioning

confidence: 80%

Section: Microbiological Analysismentioning

confidence: 95%

The impacts of water and ethanolic extracts of propolis on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans during chilly storage

Yazgan

Burgut

Durmuş

et al. 2020

Journal of Food Safety

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“…The antimicrobial activity of PE has been ascribed to the presence of bioactive compounds such as flavonoids (e.g., tectochrysin, pinobanksin, pinocembrin, chrysin, galangin, apigenin, and kaempferol), which show antibacterial effect. [42] The in vitro and in vivo antimicrobial activity of propolis against bacteria has been reported by Seibert et al [49] and Khodabakhshi et al [50] Duman and Özpolat [17] stated how bacterial growth in PE-coated Barbus grypus reached less than that in uncoated samples. The result of the present study indicated that the PE used in fresh shibuta fillet coated with gelatin leads to a reduction in microbial contamination during long storage time.…”

Section: Changes In Microbiological Analysismentioning

confidence: 83%

Shelf Life of Saurida tumbil during Storage at Refrigeration Condition as affected by Gelatin-Based Edible Coatings Incorporated with propolis Extract

Nessianpour

Khodanazary

Hosseini

2019

International Journal of Food Properties

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The aim of this study was to evaluate the effect of gelatin coating in combination with propolis extract (PE) on the microbiological (total mesophilic bacteria and psychrotrophic count), physicochemical (total volatile basic nitrogen (TVBN), 2-thiobarbituric acid reactive substances (TBARS), and free fatty acid (FFA)), and sensory characteristics in Saurida tumbil fillets during refrigerated storage (4 ± 1°C). The results showed that the gelatin-PE samples reduced the extent of lipid oxidation, as judged by TBARS and FFA, suggesting that there was a synergistic effect between gelatin coating and propolis. Moreover, the application of gelatin coatings with PE improved the TVBN and pH values of the Saurida tumbil samples significantly as compared to untreated sample, thus extending the shelf life of Saurida tumbil fillet approximately 4 days. Gelatin coating with PE was effective in inhibiting bacterial growth. The effect on the TVBN and pH indexes during storage was in accordance with bacterial activity. The gelatin-PE coatings had a positive effect on sensory acceptance of the fillets during storage. Thus, gelatin coatings enriched with PE had a better impact on the sensory characteristics and had more durability (4 days) than control. These results confirmed that gelatin coating enriched with PE can be an improved method of reducing deterioration of refrigerated Saurida tumbil fillets.

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“…Figure 9C illustrates histopathological evaluation of skin specimens from the healed wound area at 15 th www.nature.com/scientificreports www.nature.com/scientificreports/ day after wound creation. The PU/EEP-PCL/Gel group specimens exhibited significantly more developed dermis in comparison with the PU/EEP and Ctrl specimens due to presence of lower number of inflammatory cells and development of more hair follicles 75,76 . Also, the specimens were stained with Masson's trichrome to analysis collagen deposition.…”

Section: Hydrolytic and Enzymatic Degradation Hydrolytic Degradationmentioning

confidence: 95%

“…The animal experiments were done according to our previous studies 30,32 . Briefly, 24 female Wistar rats (6-8 weeks old, 150-180 g) were purchased from Pastor Institute of Tehran, Iran.…”

Section: Animal Model and Histological Evaluationmentioning

confidence: 99%

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Eskandarinia

Kefayat

Agheb

et al. 2020

Sci Rep

Self Cite

155

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one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. in the present study, polycaprolactone/gelatin (pcL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (pU/eep). the pU/eep membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells' adhesion and proliferation. the bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers' diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats' skin wound model. taking together, the pU/eep-pcL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities. Skin is always at the exposer of different types of damages 1. Severe skin damages can be life-threatening due to loss of human body fluids, electrolytes, and nutritional components from the wound area. Therefore, wound dressings have gained lots of attention 2. An ideal wound dressing should protect the wound from external contaminants and facilitate the healing process. However, one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are

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In vitro and in vivo performance of a propolis-coated polyurethane wound dressing with high porosity and antibacterial efficacy

Cited by 95 publications

References 48 publications

The impacts of water and ethanolic extracts of propolis on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans during chilly storage

The impacts of water and ethanolic extracts of propolis on vacuum packaged sardine fillets inoculated with Morganella psychrotolerans during chilly storage

Shelf Life of Saurida tumbil during Storage at Refrigeration Condition as affected by Gelatin-Based Edible Coatings Incorporated with propolis Extract

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

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