In vitro and in vivo studies of osteoblast cell response to a titanium-6 aluminium-4 vanadium surface modified by neodymium:yttrium–aluminium–garnet laser and silicon carbide paper

Abstract

The effects of neodymium:yttrium-aluminium-garnet (Nd:YAG) laser and silicon carbide (SiC) paper on the surface micro-topography of titanium-6 aluminium-4 vanadium (Ti6Al4V) alloy were examined in relation to the response of bone cells. The study was performed in three distinct stages: (1) after surface treatment of samples by laser and SiC paper, the surface hardness, surface roughness, corrosion resistance and surface tension were evaluated; (2) the growth of mouse connective tissue fibroblast cells (L-929) on untreated and treated samples was assessed in vitro; (3) the response of goat osteoblast cells to untreated and treated implanted samples was assessed in vivo. The surface roughness varied between 7 +/- 0.02 for laser-treated samples (LTSs) at 140 J cm(-2) and 21.8 +/- 0.05 for mechanically treated samples (MTSs). The surface hardness was found to vary from 377 Vickers hardness number (VHN) for MTSs to 850 VHN for LTSs. A corrosion potential of -0.21V was achieved for the LTSs compared with -0.51V for the MTSs. The LTSs exhibited a more hydrophilic behaviour (i.e. wettability) than did the MTSs. No cytotoxicity effect, unlike for the MTSs, was observed for the LTSs. The results of in vivo tests indicated longitudinal growth of osteoblast cells along the grooves on the samples formed by the SiC paper, and multidirectional spreading of the cells on the LTSs.

Fundamentals of Biomedical Applications of Biomorphic SiC

“…Also, it has been demonstrated that for primary bovine osteoblasts, the wettability is one of the key factors. In our studies , 2007; Khosroshahi et al, 2009), it is shown that the wettability of the surface can provide a better spreading condition for osteoblast cells due to reduced contact angle. Bearing in mind that the adhesion of bone cells to implant surface consists of two stages.…”

Characterization and Evaluation of Surface Modified Titanium Alloy by Pulse Nd:YAG Laser for Orthopaedic Applications: an Invivo Study

“…Also, it has been demonstrated that for primary bovine osteoblasts, the wettability is one of the key factors [Meyer, 1993]. In our studies [Khosroshahi (a) Khosroshahi et al, 2009] it is shown that the wettability of the surface can provide a better spreading condition for osteoblast cells due to reduced contact angle. Bearing in mind that the adhesion of bone cells to implant surface consists of two stages.…”

Fabrication and characterization of the microporous and nanoporous anodic oxidations of titanium–silver alloys

“…[20,21] The MTT reagent reacts with the tetrazolium ring (produced by the mitochondrial dehydrogenases of living cells) to produce a blue formazan product, which can be measured spectrophotometrically. The amount of formazan produced is proportional to the number of viable cells present.…”

“…The results from three individual experiments were averaged and the percentage of cell viability was calculated. [20,21] We also evaluated initial and long-term cell attachment for 1 day up to 7 days using MTT assay. Results were calculated as the percentage of cell attachment by its optical density (OD) values.…”

“…L-929 cells were cultured as previously described. [20] Cells were maintained in RPMI-1640 culture medium (RPMI 1640, WelGENE, Daegu, Korea) consisting of 1% penicillin-streptomycin (Pen Strep, Gibco, California, USA) and of 10% fetal bovine serum (FBS, Gibco, California, USA). The cells were cultured at 37…”