In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova, Olivera; Buschmann, Johanna

doi:10.22203/ecm.v034a02

Cited by 68 publications

(79 citation statements)

References 149 publications

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“…We analyzed the presence and amount of two isoforms of PDGF, finding that PDGF-AB increased 7 days after collection, remaining at the same level at T14. Very interestingly, PDGF-BB was released at very high levels at T0, remained at the same level at T7, and further increased at T14, suggesting that this factor is primarily responsible for the fast healing of oral wounds, in accordance with what was demonstrated by Evrova et al in tendon healing [30].…”

Section: Discussionsupporting

confidence: 86%

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Serafini

Lopreiato

Lollobrigida

et al. 2020

JCM

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Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and cell counts. The concentration of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor-AB (PDGF-AB), platelet-derived growth factor-BB (PDGF-BB), bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF) released by liquid fibrinogen were examined with ELISA at three time points (T0, time of collection; T7, 7 days; T14, 14 days). The cellular content of the liquid fibrinogen and whole blood was also calculated for each volunteer. A mean accumulation of platelets of almost 1.5-fold in liquid fibrinogen compared to whole blood samples was found. An increase of TGF-β1, PDGF-AB, FGF-2, and VEGF levels was detected at T7. At T14, the level of TGF-β1 returned to T0 level; PDGF-AB amount remained high; the levels of FGF-2 and VEGF decreased with respect to T7, but remained higher than the T0 levels; PDGF-BB was high at all time points; BMP-2 level was low and remained constant at all time points. TGF-β1, PDGF-AB, and PDGF-BB showed a correlation with platelet amount, whereas BMP-2, FGF-2, and VEGF showed a mild correlation with platelet amount. Due to the high concentration of platelets, liquid fibrinogen does contain important growth factors for the regeneration of both soft and hard tissue. The centrifugation protocol tested in this study provides a valid solution to stimulate wound healing in oral and periodontal surgery.

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Section: Discussionsupporting

confidence: 86%

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Serafini

Lopreiato

Lollobrigida

et al. 2020

JCM

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“…When PDGFR is bound by its ligands, intracellular tyrosine kinases are activated and transduce the signal further into the cell. All PDGF ligands form homodimers through disulfide bonds except PDGFA and PDGFB, which form heterodimers [26,27]. The different ligands (including AA, BB, AB, CC, and DD) differ in their affinity for the different receptors (αα, αβ, and ββ) and activate different types of downstream signaling pathways [22].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma

Lin

Yang

et al. 2020

IJMS

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Oral squamous cell carcinoma (OSCC) is a cancerous disease with poor prognosis. According to the statistics, the 5-year survival rate has not improved significantly over the past 20 years. The platelet-derived growth factor (PDGF) and its signaling pathway is a key regulator of angiogenesis and tumorigenesis. High level of PDGF and its receptor (PDGFR) have been reported in several types of malignancies. In this study, we investigated the relationship of the molecular expression levels of PDGF and PDGFR with clinicopathological parameters in OSCC. To this end, we measured the mRNA and protein levels of PDGF and PDGFR by real-time quantitative PCR (qRT-PCR), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), respectively. We found positive correlations of the mRNA levels of PDGFA, PDGFB, and PDGFRB with lymph node metastasis and poor overall survival (OS). High expression of PDGF, PDGFRA, and PDGFRB were remarkably associated with lymph node metastasis and poor OS, as determined by immunohistochemistry. Preoperative serum levels of PDGF-AA and PDGF-BB had a positive correlation with preoperative platelet count. Elevated serum levels of PDGF-AA. PDGF-BB, and platelet count correlated with lymph node metastasis and an unfavorable outcome. In multivariate Cox regression analysis, PDGFA mRNA, PDGFB mRNA, PDGFRB mRNA, PDGF immunoexpression, PDGFRB immunoexpression, serum PDGF-AA, serum PDGF-BB, and platelet count emerged as significant independent prognostic factors for OS. In vitro, we found that elevated PDGF promotes colony formation, migration, and invasiveness of SAS and OECM-1 cancer cell lines. Our results suggest that the expression level of serum PDGF has the potential to become a useful diagnostic marker for the prognosis of OSCC. In addition, PDGFR should be considered as a potential therapeutic target for OSCC. Furthermore, research should be undertaken to elucidate the role of PDGF and PDGFR regarding the behavior of tumor cells in OSCC.

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“…PDGF‐BB is a growth factor known to enhance mitogenesis, and it has been shown to support tendon healing . We therefore designed a polymer tube with incorporated PDGF‐BB and assessed it in a full‐transection rabbit Achilles tendon model.…”

Section: Discussionmentioning

confidence: 99%

“…Further development of the tube consisted in the incorporation of a growth factor, PDGF‐BB, into the electrospun mesh by spinning an emulsion . PDGF‐BB enhances tendon healing with respect to accelerated mitogenesis, chemotaxis and vascularization .…”

mentioning

confidence: 99%

Impact of PDGF‐BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post‐operation

et al. 2020

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Current methods for tendon rupture repair suffer from two main drawbacks: insufficient strength and adhesion formation, which lead to rerupture and impaired gliding. A novel polymer tube may help to overcome these problems by allowing growth factor delivery to the wound site and adhesion reduction, and by acting as a physical barrier to the surrounding tissue. In this study, we used a bilayered DegraPol® tube to deliver PDGF‐BB to the wound site in a full‐transection rabbit Achilles tendon model. We then performed histological and immunohistochemical analysis at 3 weeks postoperation. Sustained delivery of PDGF‐BB to the healing Achilles tendon led to a significantly more homogenous cell distribution within the healing tissue. Lower cell densities next to the implant material were determined for +PDGF‐BB samples compared to −PDGF‐BB. PDGF‐BB application increased proteoglycan content and reduced alpha‐SMA+ areas, clusters of different sizes, mainly vessels. Finally, PDGF‐BB reduced collagens I and III in the extracellular matrix. The sustained delivery of PDGF‐BB via an electrospun DegraPol® tube accelerated tendon wound healing by causing a more uniform cell distribution with higher proteoglycan content and less fibrotic tissue. Moreover, the application of this growth factor reduced collagen III and alpha‐SMA, indicating a faster and less fibrotic tendon healing.

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In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Cited by 68 publications

References 149 publications

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma

Impact of PDGF‐BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post‐operation

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