In vitro and in vivo trypanocidal effect of lipophilic extracts of medicinal plants from Mali and Burkina Faso

Aderbauer, Birgit; Clausen, Peter‐Henning; Kershaw, Olivia; Melzig, Matthias F.

doi:10.1016/j.jep.2008.06.024

Cited by 35 publications

(30 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is one of the most important diseases of domestic livestock in sub-Saharan Africa. The disease is most important for cattle but also pigs, camels, goats and sheep are affected (Aderbauer et al, 2008). Infections of livestock as well as companion animals like dogs with T. congolense and T. b. brucei are very common especially, in South Eastern Nigeria (Omamegbe et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Antitrypanosomal Activity of Buchholzia coriacea Seed Extract against a Field Strain of Trypanosoma congolense

Nweze

Anene

Asuzu

2011

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

The antitrypanosomal activity of the methanol extract of Buchholzia coriacea seed against a field strain of Trypanosoma congolense was investigated using experimentally infected mice of both sexes. Monitoring of parasitaemia was by the rapid matching technique. When parasitaemia was approximately log 7.8 (63 x 10 6 parasites/ml), treatment with graded doses of the extract (250, 500 and 1000 mg/kg) was instituted for 5 consecutive days. Diminazene diaceturate (Dimivet ® SKM Pharma Pvt. Ltd.) was given at 3.5 mg/kg i.p. to the positive control mice. No significant differences in body weights were observed. The rectal temperatures of infected mice showed fluctuations. The PCV of infected mice were significantly (p < 0.05) lower than those of the uninfected controls. There was no significant difference between the PCV of the extract-treated and untreated animals. Parasitaemia increased steadily in the extract-treated and untreated mice groups till all the animals died. Three days post-treatment with diminazene diaceturate parasitaemia was cleared. Six days later, there was a relapse of infection. By the end of the experiment, a 50 % relapse rate was recorded in the diminazene diaceturatetreated group. The methanol extract of Buchholzia coriacea seeds did not show any antitrypanosomal activity in mice infected with Trypanosoma congolense at the doses tested.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation of the Antitrypanosomal Activity of Buchholzia coriacea Seed Extract against a Field Strain of Trypanosoma congolense

Nweze

Anene

Asuzu

2011

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

show abstract

“…In vitro evaluation of plant extract for antitrypanosomal effect is a rapid and cheap test for evaluating the efficacy of plant against trypanosomes. Some plants had been reported to exhibit in vitro trypanocidal effect measured by cessation of motility of the parasites (Habila et al, 2011) and minimum inhibitory concentration (Freiburghaus et al, 1996;Aderbauer et al, 2008), while some were reported to exhibit trypanostatic effect measured by reduction in motility of the parasites (Atawodi, 2005). The result of this study showed that crude methanol extract of S. longipedunculata was able to immobilize the parasite completely within 75 min observation period.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the course of the disease in the animals was only slightly longer than that of the animals from in vivo study, indicating that the virulence of the pathogen was not affected. This is very important because hitherto cessation of parasite motility has been taken as trypanocidal effect (Freiburghaus et al, 1996;Aderbauer et al, 2008;Habila et al, 2011) while reduction in trypanosome motility is taken as trypanostatic (Atawodi, 2005). These conclusions should be made with caution when only in vitro test is used.…”

Section: Concentrations (Mg/ml) Pre-patent Period (Days)mentioning

confidence: 99%

Ex vivo trypanostatic effect of stem-bark extracts of Securidaca longipedunculata (Fres. Holl) against Trypanosoma brucei brucei

Tauheed¹,

Сулейман²,

Mamman³

et al. 2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Current treatment of trypanosomosis in sub-Saharan Africa is associated with widespread inefficiency.There is therefore the need to find more effective drugs against the disease from promising traditional medicinal herbs. This work is aimed at evaluating ex vivo anti-trypanosomal effect of stem-bark extracts of S. longipedunculata against T. brucei brucei. One hundred microlitre of crude methanol, ethyl acetate and aqueous methanol extracts of S. longipedunculata at concentrations of 3 and 6 mg/ml each were mixed with 50 µl of blood containing 8.1 × 10 6 trypanosomes and incubated at 37°C for 90 min. Similarly, diminazene aceturate (10 µg/ml), physiological saline solution (50 µl) and blood (100 µl) containing trypanosomes only served as treated, negative and untreated controls, respectively. Motility of the parasite was monitored under light microscope (×400) at 5 min interval throughout the 90 min observation period. All experiments were done in duplicate. The mixtures were subsequently inoculated into rats that were not previously infected with trypanosomes. Phytochemical screening of the extracts revealed the presence of carbohydrates, cardiac glycosides, saponins, steroid, triterpenes, flavonoids and tannins. However, aqueous and ethyl acetate fractions were devoid of flavonoids. The crude methanol immobilized the parasites within 75 min, while ethyl acetate and aqueous extracts induced slight reduction in motility of the parasite at 90 min of incubation. However, inoculated rats developed infection and succumbed to the infection. It is concluded that the stem-bark of the plant possesses trypanostatic, but not trypanocidal, activity against the parasite.

show abstract

“…17 Plants have been reported to be the foundation of traditional treatment for various types of disorders. 6,15,18,19 Nevertheless, there have been lately numerous reports on the assessment of the antitrypanosomal properties of plant extracts and plant derivatives. 2,18,[20][21][22][23][24] Punica granatum L., commonly recognized as the pomegranate, belongs to the family Punicaceae which comprises only one genus and two species, the other one, little-known, being P. protopunica (Balf.)…”

Section: Introductionmentioning

confidence: 99%

In vitro antitrypanosomal potential of chloroform leaf extract of Punica granatum L. on Trypanosoma brucei brucei and Trypanosoma evansi

Fathuddin

Inabo

2017

Microbiol Res (Pavia)

View full text Add to dashboard Cite

The plant Pomegranate (Punica granatum Linn.) selected for this study is native to the region of Eurasia. The objective of this study was to evaluate the antitrypanosomal potential of the plant against Trypanosoma brucei brucei (T.b. brucei) and Trypanosoma evansi (T. evansi). Similarly, the parasites used for this study have two entirely different modes of transmission that is Cyclical Transmission (T.b. brucei) and Mechanical Transmission (T. evansi). The chloroform extract of Punica granatum (P. granatum) was analysed in vitro for trypanocidal activity against T.b. brucei and T. evansi at concentrations of 100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL and 6.25 mg/mL. The chloroform extracts of P. granatum had trypanocidal activity against T. evansi and was inactive against T.b. brucei. These findings suggest that the mode of transmission may have an effect on the parasite-drug reaction and the possible use of the chloroform extract of P. granatum in the management of trypanosomiasis due to T. evansi which may require further elucidation.

show abstract

In vitro and in vivo trypanocidal effect of lipophilic extracts of medicinal plants from Mali and Burkina Faso

Cited by 35 publications

References 29 publications

Investigation of the Antitrypanosomal Activity of <i>Buchholzia coriacea</i> Seed Extract against a Field Strain of <i>Trypanosoma congolense</i>

Investigation of the Antitrypanosomal Activity of <i>Buchholzia coriacea</i> Seed Extract against a Field Strain of <i>Trypanosoma congolense</i>

Ex vivo trypanostatic effect of stem-bark extracts of Securidaca longipedunculata (Fres. Holl) against Trypanosoma brucei brucei

In vitro antitrypanosomal potential of chloroform leaf extract of Punica granatum L. on Trypanosoma brucei brucei and Trypanosoma evansi

Contact Info

Product

Resources

About