In-Vitro and In-Vivo Tools in Emerging Drug Delivery Scenario: Challenges and Updates

Tandel, Hemal; Bhatt, Priyanka; Jain, Keerti; Shahiwala, Aliasgar; Misra, Ambikanandan

doi:10.1201/b22448-1

Cited by 17 publications

(9 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo studies, though more complex than in vitro ones, demand techniques not so accessible such as PET/SPECT, but provide a more “realistic” picture of the fate of the NCs and encapsulated drugs, which has a direct impact on the evaluation of the therapeutic efficacy of the NCs and their clinical relevance. In vitro studies on the other hand are more accessible in terms of availability of techniques, which are usually fluorescence based, and provide a useful pre-screening evaluation of the potential of NCs to deliver drugs in cells and tissues, and bring insight into the fate of NCs and drugs at the cellular level, which is highly complementary to in vivo studies [ 127 ]. The advantages and disadvantages of the different in vitro and in vivo techniques presented in this manuscript have been summarized in Table 1 , highlighting which information can be obtained from each technique for the assessment of the fate of NCs and NC/drug conjugates, which we believe also hints at the importance of combining in vitro and in vivo studies.…”

Section: In Vivo Techniquesmentioning

confidence: 99%

Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate

et al. 2021

View full text Add to dashboard Cite

Nanoformulations offer multiple advantages over conventional drug delivery, enhancing solubility, biocompatibility, and bioavailability of drugs. Nanocarriers can be engineered with targeting ligands for reaching specific tissue or cells, thus reducing the side effects of payloads. Following systemic delivery, nanocarriers must deliver encapsulated drugs, usually through nanocarrier degradation. A premature degradation, or the loss of the nanocarrier coating, may prevent the drug’s delivery to the targeted tissue. Despite their importance, stability and degradation of nanocarriers in biological environments are largely not studied in the literature. Here we review techniques for tracing the fate of nanocarriers, focusing on nanocarrier degradation and drug release both intracellularly and in vivo. Intracellularly, we will discuss different fluorescence techniques: confocal laser scanning microscopy, fluorescence correlation spectroscopy, lifetime imaging, flow cytometry, etc. We also consider confocal Raman microscopy as a label-free technique to trace colocalization of nanocarriers and drugs. In vivo we will consider fluorescence and nuclear imaging for tracing nanocarriers. Positron emission tomography and single-photon emission computed tomography are used for a quantitative assessment of nanocarrier and payload biodistribution. Strategies for dual radiolabelling of the nanocarriers and the payload for tracing carrier degradation, as well as the efficacy of the payload delivery in vivo, are also discussed.

show abstract

Section: In Vivo Techniquesmentioning

confidence: 99%

Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This review will particularly focus on different targets being targeted by NPs, the possible techniques performed to conjugate NPs with targeting agents, and their biological responses in both in vitro and in vivo studies 19 .…”

Section: These Challenges Can Be Overcome As Followsmentioning

confidence: 99%

Active targeting of nanoparticles: An innovative technology for drug delivery in cancer therapeutics

Jani

Krupa

2019

J. Drug Delivery Ther.

View full text Add to dashboard Cite

In nanomedicines, currently a wide array of reported nanoparticle systems is being explored by targeting schemes which suggests great potential of targeted delivery to revolutionize cancer therapeutics. This review gives insight into recent challenges in modification of nanoparticle systems for enhanced cancer therapy acknowledged by researchers to date and also outlines different major targeting strategies of nanoparticle systems that have been utilized for the delivery of therapeutics or imaging agents, targeting ligand and cross-linking agent to cancer which was divided into three sections: 1) Angiogenesis associated targeting, 2) Uncontrolled cell proliferation targeting and 3) Tumor cell targeting. Keywords: nanoparticles, tumor cells, active targeting, targeting strategies, targeting ligands

show abstract

“…Exactly 5ml of fresh buffer was added to the dissolution vessel after each withdrawal, to maintain a constant volume. Then the withdrawal samples were analyzed by using a U.V 19,20,21…”

Section: Dissolution Studymentioning

confidence: 99%

Floating type drug delivery system: a review

Datir

Patil

Saudagar³

2019

J. Drug Delivery Ther.

View full text Add to dashboard Cite

Floating Type drug delivery system retains the dosage form for a long span. They provide local delivery to specific region like stomach and proximal small intestine and shows better bioavailability and improve therapeutic activity and substantial benefit to patients. FDDS is one of the novel drug delivery system to prolong gastric retention time. Various forms of gastro retentive drug delivery system, such as floating and non- floating. Keywords: Floating Drug Delivery System, Classification effervescent, non-effervescent, and Factors affecting FDDS.

show abstract

In-Vitro and In-Vivo Tools in Emerging Drug Delivery Scenario: Challenges and Updates

Cited by 17 publications

References 1 publication

Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate

Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate

Active targeting of nanoparticles: An innovative technology for drug delivery in cancer therapeutics

Floating type drug delivery system: a review

Contact Info

Product

Resources

About