In vitro and in vivo evaluation of Bacillus clausii against Schistosoma mansoni

Cruz, Clênio Silva; França, Wilza Wanessa Melo; Arújo, Hallysson Douglas Andrade de; Ximenes, Eulália Camelo Pessoa Azevedo; Souza, Valdênia Maria de; Albuquerque, Mônica C.P.A.; Aires, André L.; Costa, Vlaudia Maria Assis

doi:10.1016/j.actatropica.2022.106669

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…In the treatment with PZQ, the EPG reduction was 95.9% when compared to the control group, in line with the results of Cruz et al [ 80 ]. The reduction achieved in both treatments, PLUM or PZQ, can be attributed to the schistosomicidal action on whole worms and/or female worms.…”

Section: Resultssupporting

confidence: 90%

“…Our results are promising as candidates for schistosomicidal drugs that lead to the reduction in the release of eggs in feces; consequently, contributing to the restricting environmental contamination and interruption of the S. mansoni biological cycle. In the treatment with PZQ, the EPG reduction was 95.9% when compared to the control group, in line with the results of Cruz et al [80]. The reduction achieved in both treatments, PLUM or PZQ, can be attributed to the schistosomicidal action on whole worms and/or female worms.…”

Section: Effect Of Plum On Schistosoma Mansonisupporting

confidence: 89%

“…Unexpectedly, the effect of PLUM on infected mice with the S. mansoni Egyptian strain (John Bruce Egyptian strain) was recently explored by Bakery et al [65]. In this study, the mice were treated with intraperitoneal in- In the treatment with PZQ, the EPG reduction was 95.9% when compared to the control group, in line with the results of Cruz et al [80]. The reduction achieved in both treatments, PLUM or PZQ, can be attributed to the schistosomicidal action on whole worms and/or female worms.…”

Section: Effect Of Plum On Schistosoma Mansonisupporting

confidence: 83%

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Plumbagin: A Promising In Vivo Antiparasitic Candidate against Schistosoma mansoni and In Silico Pharmacokinetic Properties (ADMET)

Silva,

França,

Santos

et al. 2023

Biomedicines

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Schistosomiasis, a potentially fatal chronic disease whose etiological agents are blood trematode worms of the genus Schistosoma spp., is one of the most prevalent and debilitating neglected diseases. The treatment of schistosomiasis depends exclusively on praziquantel (PZQ), a drug that has been used since the 1970s and that already has reports of reduced therapeutic efficacy, related with the development of Schistosoma-resistant or -tolerant strains. Therefore, the search for new therapeutic alternatives is an urgent need. Plumbagin (PLUM), a naphthoquinone isolated from the roots of plants of the genus Plumbago, has aroused interest in research due to its antiparasitic properties against protozoa and helminths. Here, we evaluated the in vivo schistosomicidal potential of PLUM against Schistosoma mansoni and the in silico pharmacokinetic parameters. ADMET parameters and oral bioavailability were evaluated using the PkCSM and SwissADME platforms, respectively. The study was carried out with five groups of infected mice and divided as follows: an untreated control group, a control group treated with PZQ, and three groups treated orally with 8, 16, or 32 mg/kg of PLUM. After treatment, the Kato–Katz technique was performed to evaluate a quantity of eggs in the feces (EPG). The animals were euthanized for worm recovery, intestine samples were collected to evaluate the oviposition pattern, the load of eggs was determined on the hepatic and intestinal tissues and for the histopathological and histomorphometric evaluation of tissue and hepatic granulomas. PLUM reduced EPG by 65.27, 70.52, and 82.49%, reduced the total worm load by 46.7, 55.25, and 72.4%, and the female worm load by 44.01, 52.76, and 71.16%, for doses of 8, 16, and 32 mg/kg, respectively. PLUM also significantly reduced the number of immature eggs and increased the number of dead eggs in the oogram. A reduction of 36.11, 46.46, and 64.14% in eggs in the hepatic tissue, and 57.22, 65.18, and 80.5% in the intestinal tissue were also observed at doses of 8, 16, and 32 mg/kg, respectively. At all doses, PLUM demonstrated an effect on the histopathological and histomorphometric parameters of the hepatic granuloma, with a reduction of 41.11, 48.47, and 70.55% in the numerical density of the granulomas and 49.56, 57.63, and 71.21% in the volume, respectively. PLUM presented itself as a promising in vivo antiparasitic candidate against S. mansoni, acting not only on parasitological parameters but also on hepatic granuloma. Furthermore, in silico, PLUM showed good predictive pharmacokinetic profiles by ADMET.

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Section: Resultssupporting

confidence: 90%

Section: Effect Of Plum On Schistosoma Mansonisupporting

confidence: 89%