In vitro and in vivo evaluation of recombinant silk-elastinlike hydrogels for cancer gene therapy

Megeed, Zaki; Haider, Mohamed; Li, Daqing; O’Malley, Bert W.; Cappello, Joseph; Ghandehari, Hamidreza

doi:10.1016/j.jconrel.2003.10.027

Cited by 193 publications

(166 citation statements)

References 30 publications

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“…Mageed et al reported the use of recombinant silk-elastin-like polymer hydrogels (SELP) for the delivery of pRL-CMV for the treatment of human breast cancers. Their results suggested an increase in the transfection efficiency when SELP hydrogels were used [75]. A recent study describes encapsulation of C2C12 myoblasts in a biocompatible permselective hydrogel such as alginate-PLL-alginate (APA) to protect the cell from host immune response; while allowing diffusion of gene products.…”

Section: Application Of Hydrogels For Gene Deliverymentioning

confidence: 99%

Polymeric Hydrogels: Characterization and Biomedical Applications

Pal

Banthia

Majumdar

2009

Designed Monomers and Polymers

300

182

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Hydrogels are cross-linked polymeric networks, which have the ability to hold water within the spaces available among the polymeric chains. The hydrogels have been used extensively in various biomedical applications, e.g., drug delivery, cell carriers and/or entrapment, wound management and tissue engineering. Though far from extensive, this article has been devoted to study the common methods used for the characterization of the hydrogels and to review the range of applications of the same in health care.

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Section: Application Of Hydrogels For Gene Deliverymentioning

confidence: 99%

Polymeric Hydrogels: Characterization and Biomedical Applications

Pal

Banthia

Majumdar

2009

Designed Monomers and Polymers

300

182

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“…Encapsulation studies have shown that the presence of either charged or uncharged solutes disrupts the formation of the gel. Release studies were performed using fluorescent dextran, various proteins, and DNA [171,174]. While an increase in polymer concentration greatly reduced the release rate, the MW of the released compound had no effect on the release rate.…”

Section: Silk-elp Hybridsmentioning

confidence: 99%

“…While an increase in polymer concentration greatly reduced the release rate, the MW of the released compound had no effect on the release rate. For charged molecules such as DNA, the ionic strength of the hydrogel affects the release rate, but encapsulation does not reduce the bioactivity of the encapsulated compounds [174]. Furthermore, degradation of SELPs does not appear to produce cytotoxic intermediates, as shown by histological data [175].…”

Section: Silk-elp Hybridsmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

286

231

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Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers and other biopolymers based on repetitive peptide motifs. Applications of these engineered biomolecules in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.

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“…Many of these gels can serve as an injectable, in situ, gel-forming depot, with different DNA release kinetics depending on properties such as the polymer density, DNA conformation, extent of crosslinking, swelling ratio, and the vector affinity for the material [93][94][95]. For viral vectors, scaffold encapsulation can stabilize the vector and minimize the immune response, both of which can contribute to the long-term expression [96].…”

Section: Hydrogels-hydrogelsmentioning

confidence: 99%

Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

241

208

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Regenerative medicine aims to create functional tissue replacements, typically through creating a controlled environment that promotes and directs the differentiation of stem or progenitor cells, either endogenous or transplanted. Scaffolds serve a central role in many strategies by providing the means to control the local environment. Gene delivery from the scaffold represents a versatile approach to manipulating the local environment for directing cell function. Research at the interface of biomaterials, gene therapy, and drug delivery has identified several design parameters for the vector and the biomaterial scaffold that must be satisfied. Progress has been made towards achieving gene delivery within a tissue engineering scaffold, though the design principles for the materials and vectors that produce efficient delivery require further development. Nevertheless, these advances in obtaining transgene expression with the scaffold have created opportunities to develop greater control of either delivery or expression and to identify the best practices for promoting tissue formation. Strategies to achieve controlled localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies.

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In vitro and in vivo evaluation of recombinant silk-elastinlike hydrogels for cancer gene therapy

Cited by 193 publications

References 30 publications

Polymeric Hydrogels: Characterization and Biomedical Applications

Polymeric Hydrogels: Characterization and Biomedical Applications

Peptide-based biopolymers in biomedicine and biotechnology

Matrices and scaffolds for DNA delivery in tissue engineering

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