In Vitro and in Vivo Measurements of ROS Scavenging Activity and White Blood Cells Activity by Chemiluminescence of a New Selena-Diazole Derivative Compare to Dipyrone Activity

Abstract

Reactive oxygen species ROS scavenging activity of whole Blood and White Blood Cells were measured. The estimation depends on the principle of luminol oxidation by ROS produced during phagocytosis. The study aimed to evaluate ROS production of Whole Blood and WBCs activity. CL measured using Luminol-dependent CL reader, and the recording system is Scaler Time ST7. The female rats in four groups received T and/or Di, or received only 2mL of DW for 30 days. CL Invitro antioxidant activity the result exhibited T&Di group was significant reduction in CL. Invivo CL of T group CL activity of whole blood was significant elevated CL than all groups. While T&Di group CL activity was reduced essentially than T, Di group; Functional WBC/100cells invitro CL activity of T&Di decreased significantly than DW CL while the Di, and T groups had no important changed than DW group WBCs CL activity. The results of WBCs invivo CL were T&Di activity was significantly higher than DW activity. Also, in Di the CL activity of WBCs significantly elevated than DW. While T the WBC activity showed non-significant alteration with DW. It was concluded that T compound had a potent ROS scavenging activity with reduced WBCs activity.

Comparison of the oxidative respiratory burst and mitogen-induced leukocyte responses of camels, goats, sheep, and cows

“…The procedure for CL assay was described by Neamah et al [26] with minor modification in regard to the amount of luminophores and stimulants as well as using the whole blood in the following manner. Briefly, 96-well microtiter plates were used for the CL assay.…”