Introduction: Selena-diazole has antioxidant, and antitumor activities. Also selena-diazol exhibited promising antifungal, antibacterial, viral infection and neurodegenerative disease. The aim of the study is to evaluate the antioxidant activity of a novel -(4,5,6,7-tetrahydro- [1,2,3-] selenadiazolo [4,5 e] pyridine-4,6-diyl) bis(benzene-1,3-diol) (T) against dipyrone (Di) induced oxidative stress. Methods: In vitro antioxidant using DPPH, concentrations of T and ascorbic acid (AA) at 10, 20, 30, 40 and 50μg was measured. In vivo study conducted using four groups, received 50mg/kg of T or/and Di and DW for 30 days. Antioxidant estimated in vivo by serum superoxide dismutase activity (SOD); Glutathione Peroxidase enzyme GPx measured by using Rat SOD1 kit and Rat GPX1 ELISA Kit respectively. Furthermore, Malondialdehyde (MDA) is reliable biomarkers to predict oxidative stress. Results: The results indicate IC50 rate using DPPH of T compound 48.888μg/ml. GPx of T and T&Di groups were significantly increased. SOD of T was significantly increased than other groups. MDA results presented essential reduction in T group value than Di group. Conclusion: The study concluded that synthesized novel selena-diazole derivative T has a good effect as an anti-oxidant.

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The current study has been carried out at the department of pharmaceutical chemistry, College of pharmacy. Novel synthetically selenium-containing compounds have potential therapeutic effects towards several diseases, such as: cancer microbial infections and neurodegenerative diseases. Therefore, the present study accentuated mainly on two significant items. A novel selenadiazole derivative i.e, 4’, 4”- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) (T) and Dipyrone (Di) were used to detretmine their Biochemical effects on female rats. Biochemical test including; liver function tests; Renal functions tests; in addition lipid profile. Invivo study conducted using four groups, one as control (DW) and three treated groups (T, Di, and T&Di). The rats received 50mg/kg body weight (BW) of one of test treatments T and/or Di dissolved in 2 milliliter of distilled water and control group received same volume of distilled water for 30 days. Blood sample were collected directly from the rats heart under chloroform effect. The results indicated that Liver function test showed following results; Aspartate aminotransferase levels(AST) measurement it was cleared that only (T&Di) group (87.52 U/L ±12.20) was increased significantly than both DW(57.23 U/L ±10.43) and T(57.62 U/L ±16.54) groups. Alanine transaminase (ALT) concentration measurements showed only (T&Di) group (70.11 U/L ±13.09) value increased significantly than (DW), (T), (Di) groups. Alkaline Phosphatase (ALP) value of Di group (128.24 U/L ±27.9) highly elevated than in DW (66.68 U/L±15.29) and other test groups. Total protein (TP) concentrations of (Di) (4.97g/dL±1.02), (T) (10.87 g/dL ±3.25) and (T&Di) (5.05 g/dL ±0.76) groups highly reduced than (DW) group (14.80 g/dL ±1.98) level. Lipid profile test results show significant increase of Cholesterol (TC) level of (T) group (533.8mg/dL±52.5) than both DW (335.8mg/dL±27.01) and (T&Di) (390.3mg/dL±25.8) groups. Triglyceride (TG) serum levels only (T) group (100.1 mg/dL ±9.1) showed a significant reduction of TG value than in (Di) group (221.0572mg/dL ±39.8). Levels of HDL of (T) treated group (337.9 mg/dL±26.6) significant increased than all groups. VLDL levels results showed only Di group (43.4mg/dL±4.3) increased significantly than DW group; however there was significant decreased of T group VLDL level (20.03mg/dL±1.8) compare with Di (43.4mg/dL±4.3) and T&Di (35.9mg/dL±3.7) levels. Renal function data reveals significant reduction in blood urea levels of (T) (5.471 mg/dL ±3.745) and (T&Di) (10.633±5.431). Serum uric acid values showed significant decline of (T) group (2.601±0.743) than DW group (5.515±2.046). Also, the results of the present study illustrate only Di treated group (2.33±0.209) had essential increased of Creatinine values than all other study groups. The study concluded that synthesized novel selenadiazole derivative, and Dipyrone have mild effects on liver, kidney, and lipid profile. However, the companion of both drugs has some of undesirable effects.

Reactive oxygen species ROS scavenging activity of whole Blood and White Blood Cells were measured. The estimation depends on the principle of luminol oxidation by ROS produced during phagocytosis. The study aimed to evaluate ROS production of Whole Blood and WBCs activity. CL measured using Luminol-dependent CL reader, and the recording system is Scaler Time ST7. The female rats in four groups received T and/or Di, or received only 2mL of DW for 30 days. CL Invitro antioxidant activity the result exhibited T&Di group was significant reduction in CL. Invivo CL of T group CL activity of whole blood was significant elevated CL than all groups. While T&Di group CL activity was reduced essentially than T, Di group; Functional WBC/100cells invitro CL activity of T&Di decreased significantly than DW CL while the Di, and T groups had no important changed than DW group WBCs CL activity. The results of WBCs invivo CL were T&Di activity was significantly higher than DW activity. Also, in Di the CL activity of WBCs significantly elevated than DW. While T the WBC activity showed non-significant alteration with DW. It was concluded that T compound had a potent ROS scavenging activity with reduced WBCs activity.