In Vitro and Intracellular Production of Peptide‐Encapsulated Fluorescent Silver Nanoclusters

Yu, Junhua; Patel, Sandeep; Dickson, Robert M.

doi:10.1002/anie.200604253

Cited by 303 publications

(224 citation statements)

References 18 publications

Supporting

Mentioning

220

Contrasting

Unclassified

Order By: Relevance

“…Some biomacromolecules, such as cell-penetrating peptides (CPPs), may be capable of penetrating membranes without overt lipid bilayer disruption/poration [12][13][14][15] . Likewise, synthetic nanomaterials with very small dimensions (molecules, metal nanoclusters 16 , small dendrimers 10 and carbon nanotubes 17 ) can also pass through cell membranes. However, to the best of our knowledge, no synthetic material larger than a few nanometres in size can pass through membranes without disrupting the integrity of these biological barriers.…”

mentioning

confidence: 99%

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Verma¹,

Uzun²,

et al. 2008

Nature Mater

1,195

1,203

View full text Add to dashboard Cite

Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic material of comparable size has shown this property yet. Cationic nano-objects pass through cell membranes by generating transient holes, a process associated with cytotoxicity. Studies aimed at generating cell-penetrating nanomaterials have focused on the effect of size, shape and composition. Here, we compare membrane penetration by two nanoparticle 'isomers' with similar composition (same hydrophobic content), one coated with subnanometre striations of alternating anionic and hydrophobic groups, and the other coated with the same moieties but in a random distribution. We show that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes. Our results offer a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio-and macro-molecules. Nanomaterials are of great interest for use in biomedicine as imaging tools 1-3 , phototherapy agents 4,5 and gene delivery carriers 6,7 . Their interactions with cell membranes are of central importance for all such applications. For example, many drugdelivery systems are based on the transport of therapeutic agents to the cytosol or nucleus of cells by nanoparticles; efficient delivery must be achieved while avoiding cytotoxicity during passage through cell membranes to reach intracellular target compartments 8,9 . Indeed, membrane penetration by synthetic 10 as well as by biologically derived 11 molecules/particles is currently under intense investigation. Some biomacromolecules, such as cell-penetrating peptides (CPPs), may be capable of penetrating membranes without overt lipid bilayer disruption/poration 12-15 . Likewise, synthetic nanomaterials with very small dimensions (molecules, metal nanoclusters 16 , small dendrimers 10 and carbon nanotubes 17 ) can also pass through cell membranes. However, to the best of our knowledge, no synthetic material larger than a few nanometres in size can pass through membranes without disrupting the integrity of these biological barriers. For example, charged particles (such as cationic quantum dots or dendrimers, mostly assisted by some degree of hydrophobicity) induce transient poration of cell membranes to enter cells, a process associated with cytotoxicity 18 . Alternatively, nanoparticles have been designed to explicitly disrupt endolysosomal membranes to enter the cell by force 19 or enter the cell aided by exogenous agents such as CPP chaperones 20 . In contrast, most nanoparticles are trapped in endosomes 21 and hence do not reach the cytosol.The surface properties of nanomaterials play a critical role in determining the outcome of their interactions with cells 22 . Recently, we found that when gold nanoparticles are coated with binary mixtures of hydrophobic and hydrophilic organic mo...

show abstract

mentioning

confidence: 99%

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Verma¹,

Uzun²,

et al. 2008

Nature Mater

1,195

1,203

View full text Add to dashboard Cite

show abstract

“…[1][2][3][4] Owing to the strong quantum size confinement in this size range (o 2 nm), NCs possess discrete and size-tunable electronic transitions and display unique molecule-like properties, such as quantized charging and luminescence. [5][6][7][8][9][10][11][12] Strong luminescence is one of the most attractive features of these NCs owing to their ultrafine size, good photostability and low toxicity; 13 some of these properties may not be realized by other luminophores, such as organic dyes (for example, photostability concerns) and semiconductor quantum dots (for example, relatively large size and toxicity concerns). 2,8,9,14,15 Recent studies have shown that luminescent Au and Ag NCs are promising optical probes for bioimaging and biosensing applications.…”

Section: Introductionmentioning

confidence: 99%

Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

et al. 2013

View full text Add to dashboard Cite

This paper reports a facile aqueous-based synthesis method for highly luminescent Ag nanoclusters (NCs) with tunable emissions. The key strategy was to use a reduction-decomposition-reduction cycle to modify the Ag NC intermediates that were further subjected to size/structure focusing. The as-modified Ag NC intermediates were more robust in water against the subsequent etching by thiol ligands, making a mild size-/structure-focusing environment possible, which produced highly luminescent Ag NCs with a well-defined size and structure. Ag NCs with intense red and green emission were successfully synthesized by this method. These Ag NCs possessed a well-defined size and structure (Ag 16 (SG) 9 and Ag 9 (SG) 6 ), and exhibited excellent stability in the aqueous phase. Our highly luminescent Ag NCs also possessed superior antimicrobial properties against the multidrug-resistant bacteria Pseudomonas aeruginosa, via generating a high concentration of intracellular reactive oxygen species. Keywords: antimicrobial; biomedical applications; luminescence; silver nanoclusters; thiol ligands INTRODUCTION Ultrasmall noble metal nanoclusters (NCs), typically composed of several to a hundred metal atoms, present unique physical and chemical properties between single atoms and large nanocrystals (42 nm). [1][2][3][4] Owing to the strong quantum size confinement in this size range (o 2 nm), NCs possess discrete and size-tunable electronic transitions and display unique molecule-like properties, such as quantized charging and luminescence. [5][6][7][8][9][10][11][12] Strong luminescence is one of the most attractive features of these NCs owing to their ultrafine size, good photostability and low toxicity; 13 some of these properties may not be realized by other luminophores, such as organic dyes (for example, photostability concerns) and semiconductor quantum dots (for example, relatively large size and toxicity concerns). 2,8,9,14,15 Recent studies have shown that luminescent Au and Ag NCs are promising optical probes for bioimaging and biosensing applications. 4,[16][17][18][19][20] This finding has led to the development of various methods for the synthesis of highly luminescent Au and Ag NCs. 2,21 On the other hand, insofar as nanostructured Ag-based materials are concerned, they are finding an increasing usage for antimicrobial applications owing to the unique chemistry of Ag interfacing with microorganisms. [22][23][24] Although there is a large volume of work exploring the potential use of large Ag

show abstract

“…In general, passage across the lipid bilayer follows active or passive transport [15]. Active uptake processes follow energy-dependent endocytotic or pinocytotic pathways, while passive transport is energy-independent and characteristically reserved for small molecules/particles that diffuse through small protein channels or the lipid bilayer itself [16]. Most delivery vectors are trapped inside endosomal compartments, leading to a weak pharmacokinetic profile [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles

Carney

Mueller

et al. 2012

Biointerphases

View full text Add to dashboard Cite

Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous investigation, we showed that 66-34OT nanoparticles coated with stripe-like domains of hydrophobic (octanethiol, OT, 34%) and hydrophilic (11-mercaptoundecane sulfonate, MUS, 66%) ligands permeated through the cellular lipid bilayer via passive diffusion, in addition to endo-/pino-cytosis. Here, we show an analysis of NP internalization by DC2.4, 3T3, and HeLa cells at two temperatures and multiple time points. We study four NPs that differ in their surface structures and ligand compositions and report on their cellular internalization by intracellular fluorescence quantification. Using confocal laser scanning microscopy we have found that all three cell types internalize the 66-34OT NPs more than particles coated only with MUS, or particles coated with a very similar coating but lacking any detectable ligand shell structure, or 'striped' particles but with a different composition (34-66OT) at multiple data points.

show abstract

In Vitro and Intracellular Production of Peptide‐Encapsulated Fluorescent Silver Nanoclusters

Cited by 303 publications

References 18 publications

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles

Contact Info

Product

Resources

About