In vitro andin situ disappearance of ?-carotene and lutein from lucerne (Medicago sativa) hay in bovine and caprine ruminal fluids

Mora, Ofelia; Romano, J. L.; González, Esther Sánchez-Pardo; Ruiz, F. J.; Shimada, Armando S.

doi:10.1002/(sici)1097-0010(199902)79:2<273::aid-jsfa191>3.0.co;2-v

Cited by 13 publications

(13 citation statements)

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“…plants as well as samples rich in chlorophylls such as ruminal, duodenal and ileal content of herbivores (Mora et al 1999). Further methodological advances that allow identification and quantification of as many components as possible contribute to strengthening the knowledge on the bioavailability and physiological role of carotenoids and specially xanthophylls in ruminants, their metabolism, and the use of carotenoids as biomarkers of specific dietary exposure.…”

Section: Discussionmentioning

confidence: 41%

Development of a method to determine carotenoid composition of fresh forages

Cardinault¹,

Lyan²,

Doreau³

et al. 2008

Can. J. Plant Sci.

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Development of a method to determine carotenoid composition of fresh forages. Can. J. Plant Sci. 88: 1057Á1064. Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on b-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C 18 columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoidrich forage. The intra-day (3.5Á7.5%) and inter-day (1.2Á3.5%) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants.Key words: Xanthophylls, carotenoids, fresh forage, HPLC Cardinault, N., Lyan, B., Doreau, M., Chauveau, B., Rock, E. et Grolier, P. 2008. De´veloppement d'une me´thode afin de de´terminer la composition en carote´noı¨des de fourrages frais. Can. J. Plant Sci. 88: 1057Á1064. En raison de l'inte´reˆt limitej usqu'a`maintenant pour les carote´noı¨des dans les re´gimes des ruminants, les analyses sont souvent incomple`tes et se focalisant principalement sur le b-carote`ne et la lute´ine. La composition en carote´noı¨des d'un fourrage vert d'une prairie de moyenne montagne a e´te´analyse´e par CLHP apre`s extraction et e´limination des chlorophylles par une saponification mode´re´e. Cette me´thode d'analyse utilise deux colonnes C18 en se´rie avec un syste`me de gradient quaternaire. Notre me´thode a permis pour la premie`re fois d'identifier et de quantifier plusieurs autres xanthophylles (violaxanthine, antheraxanthine, epiluteine) que la luteine dans un extrait de fourrage riche en carote´noı¨des de´pourvu de chlorophylles Les coefficients de variations intra-jour (3,5%-7,5%) et inter-jour (1,2%-3,5%) sont adapte´s pour une analyse courante des carote´noı¨des dans les fourrages verts. Cette me´thode pourrait aussi eˆtre utilise´e dans des e´tudes me´taboliques sur ces micronutriments chez les ruminants.

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Section: Discussionmentioning

confidence: 41%

Development of a method to determine carotenoid composition of fresh forages

Cardinault¹,

Lyan²,

Doreau³

et al. 2008

Can. J. Plant Sci.

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“…b-carotene and lutein were eluted isocratically with acetonitrileÁtetra-hydrofuranÁwater (77.5:20.0:2.5, v/v/v) at a flow rate of 1 ml/min, with b-carotene detection at 436 nm and lutein detection at 474 nm. In all cases, a 20 ml aliquot was injected into the HPLC system (Mora et al 1999).…”

Section: Chemical Analysesmentioning

confidence: 99%

“…Green forages consumed by cattle contain high concentrations of carotenoids, which undergo practically no change as they pass through the rumen (Van Soest 1994;Mora et al 1999) and are deposited in the adipose tissue, causing a yellow colour to develop (Morgan & Everitt 1969;Mora et al 2000;Mora et al 2001). In bovines, the pigment b-carotene is mainly responsible for this colour, but lutein also contributes (Yang et al 1992;Mora et al 2000;Tian et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Studies on the carotenoid content in forage species and tropical beef cattle in Mexico

Barrón

González

et al. 2011

New Zealand Journal of Agricultural Research

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To determine the effect of feed carotenoids on subcutaneous fat colour in cattle, 4567 animals were sampled at an abattoir; 790 of these were chosen for this study on the basis of instrumental colour measurements. The mean concentrations of b-carotene and lutein were 0.23 (SE 0 0.008) and 0.12 (SE 0 0.004) mgg(1 adipose tissue, respectively. Sun-drying and ensiling resulted in a 97.7% and 67.4% loss of b-carotene respectively (PB0.01), but an average loss of lutein of 71.7% (PB0.1) with no difference (P!0.1) between method of preservation. A 196-day field trial was conducted using 70 cross-bred 320kg (938 kg) bulls, which were grazed on a 32 ha Brachiaria decumbens pasture and offered a commercial supplement. After 28 days, five animals were also offered sun-dried Brachiaria humidicola, while another five received it ensiled. This scheme was repeated every 28 days, until day 168, when all but 10 animals were being offered either hay or silage. There was no difference in the concentration of either carotenoid as a result of the use of hay or silage at any time (P!0.1). Processing method had a greater effect on carotenoid concentration than plant species.

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“…In our study, all steers were fed with 3.349 g d −1 ␤-carotene, this is equivalent to 347 mg ␤-carotene kg −1 DM (3339 mg ␤-carotene/9.618 kg −1 DM feed intake). In vitro and In vivo studies using either sheep or cattle reveal that ruminal microbial degradation of dietary ␤-carotene is limited, even following prolonged incubation (Dawson and Hemington, 1974;Mora et al, 1999). In the present study, ␤-carotene recovery at the proximal duodenum averaged 70.7 ± 8.2% of intake.…”

Section: Discussionmentioning

confidence: 99%

“…Of the plant chromogens involved, ␤-carotene (the most abundant of the pasture carotenoids) is the major contributor to this yellow discoloration (Mora et al, 1999). Discoloration is largely a function of pigment concentration (Kneifel et al, 1992).…”

Section: Introductionmentioning

confidence: 99%