In vitro anthelmintic activity of the crude hydroalcoholic extract of Piper cubeba fruits and isolated natural products against gastrointestinal nematodes in sheep

Carlis, Matheus Souza de Paula; Féboli, Aline; Laurentiz, Antônio Carlos de; Filardi, Rosemeire da Silva; Oliveira, Anna Helena Prizantelli de; Silva, Márcio Luís Andrade e; Anjos, Luciano Alves dos; Magalhães, Lizandra Guidi; Laurentiz, Rosangela da Silva de

doi:10.1016/j.vetpar.2019.108932

Cited by 13 publications

(6 citation statements)

References 36 publications

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“…Carlis and co-workers assessed cubebin, its oxidation product hinokinin, and dihydrocubebin for their in vitro anthelmintic activity against gastrointestinal nematodes using the egg hatch test, larval development test and L3 migration inhibition test. 57 These compounds showed ovicidal activity with EC 50 values of 150.00 μg mL −1 , 186.70 μg mL −1 and 68.38 μg mL −1 , respectively. In larval development test, cubebin showed an EC 50 value of 14.89 μg mL −1 , and an EC 50 value of 30.75 μg mL −1 for hinokinin.…”

Section: Resultsmentioning

confidence: 94%

Naturally occurring phenylethanoids and phenylpropanoids: antimalarial potential

Abdelmohsen,

Bayoumi,

Mohamed

et al. 2023

RSC Adv.

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Section: Resultsmentioning

confidence: 94%

Naturally occurring phenylethanoids and phenylpropanoids: antimalarial potential

Abdelmohsen,

Bayoumi,

Mohamed

et al. 2023

RSC Adv.

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“…The hexane partition HF1 that consisted mainly of lignans, although inactive against C. elegans, T. canis and A. caninum, was active against A. suum and T. suis larvae. Some lignans have been suggested to have nematocidal and ovicidal properties 25 . This observation suggests that the weakly anthelmintic lignans may have a contributory effect to the overall anthelmintic action of P. urinaria extracts.…”

Section: Resultsmentioning

confidence: 99%

Anthelmintic Activities of Extract and Ellagitannins from Phyllanthus urinaria against Caenorhabditis elegans and Zoonotic or Animal Parasitic Nematodes

Jato,

Waindok,

Ngnodandi

et al. 2023

Planta Med

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The aerial parts of Phyllanthus urinaria are used in traditional medicine in West Africa against helminthiasis, but their anthelmintic potential has not been evaluated until now. Within the current study, a hydroacetonic extract (AWE) and fractions and isolated ellagitannins from P. urinaria were, therefore, tested in vitro against Caenorhabditis elegans and the larvae of the animal parasites Toxocara canis, Ascaris suum, Ancylostoma caninum, and Trichuris suis. Compounds 1 – 13, mainly representing ellagitannins, were isolated using different chromatographic methods, and their structures were elucidated by HR-MS and 1H/13C-NMR. AWE exerted concentration-dependent lethal effects (LC50 of 2.6 mg/mL) against C. elegans and inhibited larval migration of all animal parasites tested (T. suis L1 IC50 24.3 µg/mL, A. suum L3 IC50 35.7 µg/mL, A. caninum L3 IC50 112.8 µg/mL, T. canis L3 IC50 1513.2 µg/mL). The anthelmintic activity of AWE was mainly related to the polar, tannin-containing fractions. Geraniin 1, the major ellagitannin in the extract, showed the strongest anthelmintic activity in general (IC50 between 0.6 and 804 µM, depending on parasite species) and was the only compound active against A. caninum (IC50 of 35.0 µM). Furosin 9 was least active despite structural similarities to 1. Among the parasites tested, Trichuris suis L1 larvae turned out to be most sensitive with IC50 of 0.6, 6.4, 4.0, 4.8, and 2.6 µM for geraniin 1, repandusinic acid A 3, punicafolin 8, furosin 9, and phyllanthusiin A 10, respectively.

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“…Furthermore, it is known that praziquantel also causes morphological changes in the tegument of schistosomes 42 , 53 . Studies on anthelmintic activity of other lignans were described by de Paula Carlis and colleagues 54 , in a study which evaluated the effects of the hinokinin, cubebin, and dihydrocubebin isolated from Piper cubeta fruits, against eggs and third stage larvae of sheep gastrointestinal nematodes ( Haemonchus contortus and Trichostrongylus sp). The authors reported that lignans caused serious lesions on the surface of parasites that led to death.…”

Section: Resultsmentioning

confidence: 99%

Neolignans isolated from Saururus cernuus L. (Saururaceae) exhibit efficacy against Schistosoma mansoni

Brito

Wilairatana

Roquini

et al. 2022

Sci Rep

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Schistosomiasis, a parasitic disease caused by the blood fluke of the genus Schistosoma, affects over 230 million people, especially in developing countries. Despite the significant economic and public health consequences, only one drug is currently available for treatment of schistosomiasis, praziquantel. Thus, there is an urgent demand for new anthelmintic agents. Based on our continuous studies involving the chemical prospection of floristic biodiversity aiming to discover new bioactive compounds, this work reports the in vitro antiparasitic activity against Schistosoma mansoni adult worms of neolignans threo-austrobailignan-6 and verrucosin, both isolated from Saururus cernuus L. (Saururaceae). These neolignans showed a significant in vitro schistosomicidal activity, with EC50 values of 12.6–28.1 µM. Further analysis revealed a pronounced reduction in the number of S. mansoni eggs. Scanning electron microscopy analysis revealed morphological alterations when schistosomes were exposed to either threo-austrobailignan-6 or verrucosin. These relevant antischistosomal properties were accompanied by low cytotoxicity potential against the animal (Vero) and human (HaCaT) cell lines, resulting in a high selectivity index. Considering the promising chemical and biological properties of threo-austrobailignan-6 and verrucosin, this research should be of interest to those in the area of neglected diseases and in particular antischistosomal drug discovery.

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In vitro anthelmintic activity of the crude hydroalcoholic extract of Piper cubeba fruits and isolated natural products against gastrointestinal nematodes in sheep

Cited by 13 publications

References 36 publications

Naturally occurring phenylethanoids and phenylpropanoids: antimalarial potential

Naturally occurring phenylethanoids and phenylpropanoids: antimalarial potential

Anthelmintic Activities of Extract and Ellagitannins from Phyllanthus urinaria against Caenorhabditis elegans and Zoonotic or Animal Parasitic Nematodes

Neolignans isolated from Saururus cernuus L. (Saururaceae) exhibit efficacy against Schistosoma mansoni

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