In vitro anthelmintic and cytotoxicity activities the Digitaria insularis (Poaceae)

Santos, Francianne Oliveira; Lima, Hélimar Gonçalves de; Santos, Nathália Silva de Souza; Serra, Taiane Menezes; Uzêda, Rosângela Soares; Reis, Isabella Mary Alves; Botura, Mariana Borges; Branco, Alexsandro; Batatinha, Maria José Moreira

doi:10.1016/j.vetpar.2017.08.007

Cited by 20 publications

(14 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3,4,5-Trimethoxycinnamic acid (Peak B21 , m / z 239.0915 [M + H] + ), exhibiting fragments at m / z 224.0684 [M + H − 15] + (loss of a methyl) and 195.1019 [M + H − 44] + (loss of a CO 2 molecule), was identified based on the literature [ 36 ]. Peak B22 ( m / z 331.0796 [M + H] + ), with fragments at m / z 316.0568 [M + H − 15] + (loss of a methyl) and 301.0340 [M + H − 30] + (loss of two methyls), was tentatively assigned as tricin [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

et al. 2018

View full text Add to dashboard Cite

To provide further insights into the potential health-promoting antioxidants from wild rice (Zizania latifolia), which is an abundant but underutilized whole grain resource in East Asia, a partial purification based on D101 macroporous resin was carried out for the purification and enrichment of the antioxidants from the bioactive ethanol extracts of wild rice. On that basis, 34 phenolic compounds in the antioxidant fractions were identified by a high-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC-LTQ-Orbitrap-MSn). The results suggested that phenolic acids could be enriched in the 10% ethanol-eluted fraction whereas flavonoids (including procyanidins and flavonoid glycosides) could be enriched in 20–30% ethanol-eluted fractions. A quantitative analysis determined by the multiple reaction monitoring mode of the ultra-performance liquid chromatography-triple quadrupole-tandem mass spectrometry (UPLC-QqQ-MS/MS) revealed a high content of procyanidins in wild rice. Compared with phenolic acids, flavonoids may contribute more to the potent antioxidant activity of wild rice. This is the first study on the antioxidants from wild rice Z. latifolia. These findings provide novel information on the functional components of wild rice, and will be of value to further research and development on Z. latifolia.

show abstract

Section: Resultsmentioning

confidence: 99%

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

et al. 2018

View full text Add to dashboard Cite

show abstract

“…2013; Santos et al . 2017). Despite the variety of investigations about the anthelmintic activity of plant extracts containing flavonoids, the data on the action of these isolated compounds are still scarce (Kerboeuf et al .…”

Section: Discussionmentioning

confidence: 99%

In vitroovicidal and larvicidal activities of some saponins and flavonoids against parasitic nematodes of goats

Santos

Lima

et al. 2018

Parasitology

Self Cite

View full text Add to dashboard Cite

This study assessed the anthelmintic activity of plant-derived compounds against gastrointestinal nematodes of goats using the egg hatch and larval motility assays. The compounds tested were saponins (digitonin and aescin) and their respective sapogenins (aglycones), hecogenin acetate and flavonoids (catechin, hesperidin, isocordoin and a mixture of isocordoin and cordoin). Additionally, cytotoxicity of active substances was analysed on Vero cell through 3-4,5-dimethylthiazol-2-yl,2,5diphenyltetrazolium bromide (MTT) and propidium iodide (PI) tests. Significant reduction on the egg hatching (P 90%). Nevertheless, higher cytotoxicity was observed in the MTT assay, with IC50 of 0.20 mg mL-1 (aescin) and 0.0074 mg mL-1 (digitonin). Aescin and digitonin have a pronounced in vitro anthelmintic effect and the glycone portion of these saponins plays an important role in this activity.

show abstract

“…The author also concluded that the tannins are not the only metabolites secondary involved in the antiparasitic effects of the extract. In other study, Santos et al (2017) evaluated extracts and fractions from Digitaria insularis leaves against eggs of H. contortus and obtained IC 50 values ranging from 0.27 mg mL -1 to 0.96 mg mL -1 , however the nematocidal activity of this plant was attributed to the presence of flavones.…”

Section: Resultsmentioning

confidence: 99%

In vitro ovicidal and larvicidal activity of Psidium cattleianum Sabine leaves against gastrointestinal nematodes of naturally infected sheep

Piza¹,

Féboli²,

Augusto³

et al. 2019

Bol. Ind. Anim.

View full text Add to dashboard Cite

Gastrointestinal nematodes can affect sheep productivity via severe weight loss, anemia and death. Infection control is generally performed with synthetic anthelmintic compounds; however, indiscriminate use of these drugs can stimulate the emergence of resistant nematodes. Thus, alternatives to the use of synthetic anthelmintic compounds have been proposed, such as the inclusion of medicinal plants in animal feed. The objective of this study was evaluate the anthelmintic activity of the leaves of the medicinal plant Psidium cattleianum Sabine (Araçá) through its hydroalcoholic extract, and using in vitro assays with the eggs and larvae of gastrointestinal nematodes obtained from naturally infected donor sheep. The extract exhibited good in vitro ovicidal and larvicidal activity, with IC 50 values of 0.55 mg mL-1 for egg hatching inhibition, 0.20 mg mL-1 for larval development inhibition and an efficiency greater than 80% in the inhibition of larval migration at all concentrations evaluated (IC 50 < 0.19 mg/mL). Phytochemical analysis detected higher concentrations of saponins, flavonoids, cardiac glycosides, anthraquinones and tannins in the extract. Our results demonstrated that the Psidium cattleianum Sabine's leaves exhibit in vitro anthelmintic activity, which suggests that in addition to its other medicinal properties, this plant can help control gastrointestinal nematodes in sheep. Future in vivo assays should be performed to confirm antiparasitic efficacy.

show abstract

In vitro anthelmintic and cytotoxicity activities the Digitaria insularis (Poaceae)

Cited by 20 publications

References 31 publications

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

In vitroovicidal and larvicidal activities of some saponins and flavonoids against parasitic nematodes of goats

In vitro ovicidal and larvicidal activity of Psidium cattleianum Sabine leaves against gastrointestinal nematodes of naturally infected sheep

Contact Info

Product

Resources

About