In vitro anthelmintic effect of Vicia pannonica var. purpurascens on trichostrongylosis in sheep

Kozan, Esma; Anul, Serap Arabaci; Tatlı, I. İrem

doi:10.1016/j.exppara.2013.03.018

Cited by 30 publications

(19 citation statements)

References 34 publications

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“…The phenolic acid contents for wormwood and the mix (but not for mallow) were within the range of 3.6 to 57.3 g/kg DM, as was reported for plant mixtures used previously in infected lambs [16,17]. In relation to flavonoids with antioxidant properties, we identified mainly flavones (apigenin and luteolin), flavonols (kaempferol and quercetin) and flavanones (naringenin) [28], which may also have anthelmintic activity [3,29]. However, the total content of flavonoids in wormwood in the present study was lower (0.35 g/kg DM) versus mallow or the mix (6.48 or 5.51 g/kg DM, respectively) or compared to previous studies (9.96 and 29.5 or 41.5 g/kg DM, respectively) [16,17].…”

Section: Discussionsupporting

confidence: 81%

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

Mravčáková

Komáromyová

Babják

et al. 2020

Animals

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The objective of this study is to evaluate the effect of dry wormwood and mallow on the gastrointestinal parasite of small ruminants Haemonchus contortus. Twenty-four experimentally infected lambs were randomly divided into four groups of six animals each: unsupplemented lambs, lambs supplemented with wormwood, lambs supplemented with mallow and animals supplemented with a mix of both plants. Faecal samples from the lambs were collected on day 23, 29, 36, 43, 50, 57, 64 and 75 post-infection for quantification of the number of eggs per gram (EPG). The mix of both plants contained phenolic acids (10.7 g/kg DM) and flavonoids (5.51 g/kg DM). The nematode eggs were collected and in vitro egg hatch test was performed. The aqueous extracts of both plants exhibited strong ovicidal effect on H. contortus, with ED50 and ED99 values of 1.40 and 3.76 mg/mL and 2.17 and 5.89 mg/mL, respectively, in the in vitro tests. Despite the great individual differences between the treated lambs in eggs reduction, the mean EPG of the untreated and treated groups did not differ (p > 0.05). Our results indicate that using wormwood and mallow as dietary supplements do not have a sufficient effect on lambs infected with H. contortus.

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Section: Discussionsupporting

confidence: 81%

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

Mravčáková

Komáromyová

Babják

et al. 2020

Animals

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“…Results from the present study confirm the anthelmintic properties against H. contortus previously reported for rutin [ 23 ]. On the contrary, in the case of quercetin results obtained in the present study differ from those previously reported by Kozan et al (2013) [ 24 ]. However, in this latter study quercetin was used at a higher concentration than that evaluated in the present study.…”

Section: Discussioncontrasting

confidence: 99%

“…More specifically, Garcia et al (2003) [ 22 ] reported the in vivo anthelmintic activity of mangiferin on Trichinella spiralis . Rutin and quercetin demonstrated in vitro anthelmintic activity on sheep trichostrongyles in the studies of Barrau et al [ 23 ] and of Kozan et al [ 24 ], respectively. Finally, Villaseñor et al reported the in vitro efficacy of β-sitosterol against Ascaris suum [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes

Giovanelli

Mattellini

Fichi

et al. 2018

Veterinary Sciences

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By using the egg hatch test (EHT), the larval development test (LDT) and the larval mortality/paralysis test (LMT), the in vitro anthelmintic activity on sheep gastrointestinal strongyles (GIS) of four plant-derived pure compounds, mangiferin (at 0.25%, 0.125% and 0.0625%), rutin (at 1%, 0.75%, 0.5%), quercetin (at 1%), and β-sitosterol (at 1%, 0.75%, 0.5%), was investigated. For comparison, untreated and treated (0.1% thiabendazole, 0.1% TBZ) controls were used. Six repetitions were made throughout the experiment. Data were statistically elaborated using the χ2 test. The concentration able to inhibit the development of the 50% of L1s to L3s and causing the mortality of the 50% of L3s (EC50) was also calculated. L3s recovered from untreated Petri dishes were identified at the genus level. In EHT, all tested compounds at all concentrations significantly (p < 0.01) inhibited the hatch of the eggs when compared to the untreated controls, but none of them was as effective as 0.1% TBZ. In LDT, rutin (at 1%, 0.75% and 0.5%), mangiferin (at 0.25% and 0.125%), β-sitosterol (at 1%) and 0.1% TBZ completely prevented the larval development from L1 to L3 in respect to the untreated controls (p < 0.01). In LMT, all tested compounds significantly (p < 0.01) increased the death of L3s compared to the untreated controls, except for β-sitosterol at 0.5%. However, only rutin at all concentrations and 0.25% and 0.125% mangiferin were as effective as 0.1% TBZ. Haemonchus, Trichostrongylus, Chabertia and Teladorsagia/Ostertagia GIS genera, were identified.

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“…The literature survey performed on the antiparasitic activity of the identified compounds (1-8) revealed the antiparasitic activity of quercetin, kaempferol, astragalin, tiliroside, and quercetin-3-O- glucoside against the unicellular parasitic protozoa belonging to the Trypanosoma genus, known to parasitize animals and humans [24][25][26]. Kaempferol and astragalin were reported to be the most potent anthelmintic agents (vermicides) against several animal parasite worms such as Fasciolopsis buski, Fasciola gigantical, Taenia solium, Schistosoma mansoni, or Pheretima posthuma [27][28][29], whereas quercetin-3-O-glucoside was found to possess significant anthelmintic activity against trichostrongylose larvae present in sheep [30]. Hence, it is worth hypothesizing that the ungulates preference for H. nummularium could be explained by the anthelmintic effect of the reported compounds that animals use for selfmedication.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and Biological Investigation of Helianthemum nummularium, a High-Altitude Growing Alpine Plant Overrepresented in Ungulates Diets

et al. 2020

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Abstract Helianthemum nummularium is a European shrub growing at high altitude where it copes with a high level of stress. It was found to be overexpressed in ungulates diets compared to more abundant surrounding plants. These elements combined with the fact that H. nummularium from the Alps has never been investigated prompted us to study the phytochemical composition of its aerial parts. The analysis of the polar extract allowed for the isolation of eight compounds: p-hydroxybenzoic acid, tiliroside, kaempferol, astragalin, quercetin, plantainoside B, quercetin-3-O-glucoside, and quercetin-3-O-glucuronide. We investigated the effect of the polar extract and isolated compounds on nuclear factor erythroid 2-related factor 2 transcription factor, which regulates the expression of a wide variety of cytoprotective genes. We found that the ethanolic extract activates the expression of nuclear factor erythroid 2-related factor 2 in a dose-dependent manner, whereas the pure compounds were much less active. The activation of the nuclear factor erythroid 2-related factor 2 pathway by the plant extract could pave the way for studies to promote healthy aging through protection of cells against oxidative stress. Moreover, the isolated compounds could be investigated alone or in combination in the perspective of making the link between the ungulateʼs preference for this plant and possible use of it for self-medication.

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In vitro anthelmintic effect of Vicia pannonica var. purpurascens on trichostrongylosis in sheep

Cited by 30 publications

References 34 publications

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes

Phytochemical and Biological Investigation of Helianthemum nummularium, a High-Altitude Growing Alpine Plant Overrepresented in Ungulates Diets

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