In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions

Abstract

Context: Premature aging usually occurred due to free radicals reducing the skins’ physiological functions. Muntingia calabura, a plant containing rich antioxidants, has the potential to overcome this problem. Aims: To evaluate the antioxidant capacity of M. calabura in inhibiting the premature aging process, to be potentially developed into an antiaging active ingredient. Methods: The samples were extracted using ethanol 96%, and processed into n-hexane, ethyl acetate, and ethanol fractions, respectively. Total phenolic content was determined, followed by the evaluation of antioxidant capacity through DPPH, FRAP, and ABTS assay. Further, anti-elastase was conducted using human neutrophil elastase as a skin degradation enzyme, followed by an anti-collagenase test. Finally, normal cell proliferation was also evaluated via the MTT method measuring cell viability on HDFa cells. Results: As the results, ethanol extract, ethyl acetate fraction, and ethanol fraction showed a strong antioxidant effect, having great capacity reducing DPPH, ABTS radicals, and also iron reduction, in contrast to n-hexane fraction that exhibited only weak activity. The antioxidant trend capacities were found directly correlated to total phenolic contents. Furthermore, the ethyl acetate fraction was found to have optimum activity in inhibiting elastase and collagenase enzymes, showing a similar impact on cell viability. Conclusions: The ethyl acetate fraction from M. calabura exhibits the prospect for further development to support its effectiveness as an active ingredient in antiaging cosmetics.

Pharmacognostical and Phytochemical Studies and Biological Activity of Curculigo latifolia Plant Organs for Natural Skin-Whitening Compound Candidate

“…The DPPH• radical reduction method was assayed based on a method described by Nur et al [ 27 ] with slight modification. A series of samples (10–1000 µ g/mL) was prepared.…”

Aktivitas Anti-elastase dan Antioksidan dari Ekstrak Etanol Kayu Bangkal (Nauclea subdita) Korth. Steud. dengan Variasi Metode Ekstraksi

“…Sel fibroblas akan mengalami penurunan populasi seiring bertambahnya usia. Penurunan populasi sel fibroblas menyebabkan penurunan biosintesis kolagen pada lapisan dermis yang dapat menyebabkan kerutan pada kulit serta mengarah pada proses penuaan (Nur et al, 2021).…”

“…Metode Folin-Ciocalteu digunakan untuk penentuan fenolik total, dengan asam galat digunakan sebagai standar, dengan sedikit modifikasi (Nur et al, 2021). Pada pengujian sampel,dan standar dilakukan pemipetan sebanyak 200 μL larutan uji yang sesuai dan masing-masing seri larutan standar ke dalam wadah yang sesuai.…”

“…Reaksi MTT dihentikan dengan reagen stopper (SDS 10% dalam HCl 0,01 N), kemudian diinkubasi semalaman pada suhu kamar dan di tempat gelap. Keesokan harinya, serapan dibaca dengan microplate reader pada 595 nm (Nur et al, 2021). (2,2'-azino-bis-3ethylbenzthiazoline-6-sulfonic acid) dilakukan menggunakan spektrofotometri UV-VIS dengan standar asam askorbat dengan sedikit modifikasi (Nur et al, 2021) (Apak et al, 2010).…”

Standardization and Gc-MS Analysis of Kersen (Muntingia Calabura L.) Fruit Ethanol Extract as an Herbal Raw Material

“…Kersen fruit (Muntingia calabura L) is a plant from the Muntiangiaceae family which has some scientific evidence regarding the activity possessed by kersen fruit, namely antioxidant 5&6 , Has a protective effect against UV exposure 7 , anti-aging 8 , antibacterial 9&10 , anti-inflammatory 11 . Besides having good activity, Kersen fruit also has a variety of nutritional content 12 and compounds 7,9,[13][14][15] which can support the activity of kersen fruit.…”