In vitro anti-cholesterol and anti-hypertensive activity of stem bark the Flacourtia rukam

Abstract

Flacourtia rukam belongs to family Salicaceae and Indonesia is known as rukem. F. rukam is popular among people especially Musi Banyuas in South Sumatera, Indonesia for the treatment of hypertensive. Investigated effect anti-cholesterol and anti-hypertensive activity from extracts the stem bark of F. rukam have been done. The anti-cholesterol activity was measured by the photometric method by reaction cholesterol with Liebermann-Burchard reagent and anti-hypertensive activity using Angiotensin Converting Enzyme (ACE) inhibitory method. The crude ethanol extract showed the highest anti-cholesterol activity compared to the fractions with IC50 value of 157.88 mg/L. Crude ethanol extract also contained the highest phenolic and flavonoid content compared to fractions. The anti-cholesterol activity of extracts is equivalent to the total phenolic and flavonoid contained. In an anti-hypertensive activity study, the crude ethanol extracts exhibited the percentage of ACE inhibitory activity with IC50 119.82 mg/L. The results indicated that the stem bark of F. rukam might reduce or control the cholesterol levels and blood pressure. Anti-cholesterol and anti-hypertensive activity the stem bark of F. rukam is higher in extract form compared to fractions (synergistic).

Syzygium nervosum extraction and potent bioactivities

“…A blank sample was prepared using chloroform, and a negative control was prepared using a 100 mg/L cholesterol solution. Simvastatin (200-1000 μg/mL) was used as a positive control [10]. The remaining cholesterol in the sample was determined by measuring the absorbance at 410 nm using a spectrophotometer.…”

“…The remaining cholesterol in the sample was determined by measuring the absorbance at 410 nm using a spectrophotometer. Anti-cholesterol concentration was calculated according to the following formula [10]:…”