In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia

Keskes, Henda; Mnafgui, Kais; Hamden, Khaled; Damak, Mohamed; Feki, Abdelfattah El; Allouche, Noureddine

doi:10.12980/apjtb.4.201414b114

Cited by 68 publications

(42 citation statements)

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“…Its leaves are used in the form of a decoction to cure many diseases such as diarrhoea, bronchitis, rheumatism (Madar 1989;Bellakhder 1997), and diabetes (Amer et al 1994;Allali et al 2008). Most of the works reported in the literature to date have focused on the chemical composition determination of J. phoenicea essential oil (Adams et al 1996;Rezzi et al 2001;Cavaleiro et al 2001;Barrero et al 2006;Keskes et al 2014), whereas only limited data are reported on the nonvolatile components of leaves and/or twigs of other juniper varieties (Nakanishi et al 2002(Nakanishi et al , 2005(Nakanishi et al , 2004. These studies have shown that the main identified classes of metabolites are flavonoids and biflavonoids.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we evaluated the antioxidant property of various extracts from J. phonicea leaves and the in vitro inhibitory activity against a-amylase and lipase (Keskes et al 2014). This work investigates, for the first time, the chemical composition of hexane extract from J. phonicea leaves and of active fractions obtained from the methanol extract fractionation using gas chromatography coupled with mass spectroscopy (GC-MS) and high-performance liquid chromatography coupled with mass spectroscopy (HPLC-MS) techniques, respectively.…”

Section: Introductionmentioning

confidence: 99%

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LC-MS–MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves

Keskes

Belhadj

Jlail

et al. 2016

Pharmaceutical Biology

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Context: Despite some studies related to Juniperus phoenicea L. (Cupressaceae), phytochemical and biological investigations of this plant remain unexplored. Objective: This work is the first report dealing with the identification and characterization of volatile components and flavonoids in hexane and methanol extracts from J. phoenicea leaves Materials and methods: Antioxidant activity of hexane, and methanol extracts from J. phoenicea leaves were determined by DPPH-radical scavenging assay. a-Amylase inhibitory activity was evaluated by enzyme inhibition using in vitro assay (each extract was dissolved in DMSO to give concentrations of 50, 100 and 200 mg/mL). The chemical composition of fractions (Fr1-Fr3) from methanol extract was determined by high-performance liquid chromatography coupled with mass spectroscopy (HPLC-MS) analysis. Results and discussion: The hexane extract was analyzed by GC-MS technique which allowed the identification of 32 compounds. The main constituents were a-humulene (16.9%), pentadecane (10.2%) and a-cubebene (9.7%). Fraction Fr 2 exhibited a strong DPPH radical-scavenging activity (IC 50 ¼ 20.1 lg/mL) compared to that of BHT as well as the highest a-amylase inhibitory activity (IC 50 ¼ 28.4 lg/mL). Three flavonoids were identified in these fractions using HPLC-MS analysis: Quercetin 3-O-glucoside, isoscutellarein 7-O-pentoside and quercetin 3-O-pentoside. In addition, the more active fraction (Fr 2) was purified with semi-preparative HPLC affording one pure compound (amentoflavone) using 1 H NMR analysis. This compound exhibited powerful DPPH radical-scavenging (IC 50 ¼ 14.1 lg/mL) and a-amylase inhibition (IC 50 ¼ 20.4 lg/mL) effects. Conclusion: This study provides scientific support to some medicinal uses of J. phoenicea found in North Africa. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LC-MS–MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves

Keskes

Belhadj

Jlail

et al. 2016

Pharmaceutical Biology

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“…Various studies were conducted on essential oil composition, phenolic composition and biological activities of J. phoenicea (Medini et al, 2013a;Medini et al, 2013b;Keskes et al, 2014), but till now no morphometric study was conducted on the leaves and cones of Tunisian J. phoenicea despite their large morphological variability. Previously, the biochemical polymorphism based on the essential oil chemical composition of J. phoenicea leaves collected from various localities in Tunisia was studied and existence of two clusters (the continental localities are clearly separated from the coastal ones) was deduced.…”

Section: Introductionmentioning

confidence: 99%

Intraspecific variation and morphometric analysis of Juniperus phoenicea L. branches and cones from Tunisia

Medini¹,

Khouja²,

Chemli³

et al. 2016

JEBAS

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KEYWORDS Juniperus phoenicea Branches Cones Morphometry Taxonomy ABSTRACTThe intra-and inter populational geographic variation of eight distant populations of Juniperus phoenicea L. originating from Tunisia was examined biometrically on the basis of morphological-key characters of branches and cones. Seven and eight qualitative and quantitative (measured) traits of each branches and cones were studied on 45 and 29 vouchers, respectively. The principal component analysis (PCA) and hierarchical cluster analysis (HCA) of euclidean distances were performed to verify the hypothesis of geographic differentiation and for the existence of two J. phoenicea L. subspecies. The sampled populations differed significantly from one another, while the intra-populational variation was lower. Two major groups emerged on the PCA and HCA results, separating the continental populations from the coastal ones. Compared to the morphological traits of J. phoenicea subspecies in the literature and the basic flora, we confirm the existence of two J. phoenicea taxa: J. phoenicea ssp. turbinata was the reported from the coastal sites and J. phoenicea ssp. phoenicea located in the continental ones.

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“…These results showed that Z. album extracts may be suggested as a potential source of natural phenolic compounds which are endowed with Table III Anti-oxidant activity of of some extracts from Zygophyllum album. L e t h a n e E t h y l a c e t a t e A c e t o n e B u t a n o l E t h a n o l M e t h a n o l several activities especially anti-oxidant activity (Keskes et al, 2014). On the other hand, plants have been used traditionally to enhance cognitive function and to alleviate other symptoms associated nowadays with Alzheimer's disease (Natarajan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Anti-oxidant and anti-acetylcholinesterase activities of Zygophyllum album

Kchaou

Salah

Mhiri

et al. 2015

Bangladesh J Pharmacol

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The present study was carried out to explore the anti-acetylcholinesterase activity and anti-oxidant effect as well as content of phenolic compounds of various extracts from Zygophyllum album. It was observed that dichloromethane and ethanol extracts were endowed with a powerful anti-acetylcholinesterase activity with IC50 values of 40 and 58 µg/mL, respectively. These two extracts exhibited the highest DPPH radical scavenging activity (IC50=0.2 mg/mL), the highest total anti-oxidant capacity (185.2 and 222.4 mg vitamin E/mg extract, respectively), the highest reducing power effect, and the highest β-carotene bleaching capacity. The findings showed that the extents of anti-acetylcholinesterase activity of Z. album extracts as well as their anti-oxidant capacity are in accordance with their phenolic contents. Hence, Z. album leaves would be useful against Alzheimer's disease. VIDEO CLIP<a href="https://www.youtube.com/v/M03zi4Mzh6k">Determination of flavonoids content:</a> 2 min 50 sec

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In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia

Cited by 68 publications

References 28 publications

LC-MS–MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves

LC-MS–MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves

Intraspecific variation and morphometric analysis of Juniperus phoenicea L. branches and cones from Tunisia

Anti-oxidant and anti-acetylcholinesterase activities of <i>Zygophyllum album</i>

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