In Vitro Antibacterial Activities and Brine Shrimp Lethality Bioassay of Ethanolic Extract From Moringa Oleifera Lam. Leaves

Rafshanjani, Abu Shuaib; Parvin, Shahnaj; Kader, Abdul

doi:10.7897/2230-8407.0511175

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…The results are in correspondence to the previously conducted studies in which the lethal dose of the ethanol fraction was 8.12 μg/mL when compared to the standard vincristine, which exhibited a value of 6.76 μg/mL. 20,21 In the case of the extracts of leaves, the LD 50 value increased as the polarity of the solvent was increased. The range of LD 50 values for leaves was 24.3 μg/mL to 55.6 μg/mL.…”

Section: Cytotoxicity: Brine Shrimp Lethality Assaysupporting

confidence: 88%

Cytotoxic, in vitro Enzyme Inhibitory Potential and Phytochemical Profiling of Odontonema strictum

Nadeem

Khan

Koala

et al. 2023

Pharmacognosy Magazine

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Background Odontonema strictum is an herbaceous plant, indigenous to Central America and Central Africa, and has been used traditionally by the local community against various ailments. The current piece of work was carried out to evaluate the cytotoxic potential of O. strictum by using brine shrimp lethality and the in vitro cytotoxicity/viability assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]), as well as the in vitro enzyme inhibitory potential by using alpha-amylase, protein kinase enzymes and phytochemical analysis of the different extracts of aerial parts of O. strictum. Materials and Method Extracts of aerial parts of O. strictum were prepared through the successive extraction method using solvents with the order of polarity as n-hexane, dichloromethane, ethyl acetate, methanol and water. Cytotoxicity was assessed through brine shrimp lethality assay and in vitro cell viability assay (MTT), whereas enzyme inhibition potential was evaluated through Alpha-amylase and protein kinase assay. Phytochemical analysis was done by assessing the total phenolic and total flavonoid contents of both leaves and flowers. Results All the extracts exhibited various phytochemical and biological activities. Remarkable toxicity profiles against the brine shrimp lethality assay (LD50:11.5 µg/mL) were shown by the leaf extracts, whereas both leaves and flowers exhibited promising anti-proliferative activity, assessed by the MTT assay. Significant alpha-amylase and protein kinase inhibition potential were manifested by leave extracts with IC50:195.6 µg/mL and minimum inhibitory concentration (MIC): 16.3 µg/mL respectively. The phytochemical analysis of the plant showed that it was sufficiently rich in phenolic compounds with a total phenolic content and total flavonoid content (96.8 µg GAE/mg and 65.1 µg QE/mg, respectively). Conclusion It is plausible to believe that the cytotoxic and enzymatic activity of the aerial parts of O. strictum aerial parts might be, at least partly, to the presence of phenolic chemicals and could be a possible source of cytotoxic agent and an enzyme inhibitor.

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Section: Cytotoxicity: Brine Shrimp Lethality Assaysupporting

confidence: 88%

Cytotoxic, in vitro Enzyme Inhibitory Potential and Phytochemical Profiling of Odontonema strictum

Nadeem

Khan

Koala

et al. 2023

Pharmacognosy Magazine

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“…No mortality was observed in animal group treated with n-hexane extract. In a previous study [10] , the ethanol extract of Moringa oleifera Lam at a concentration of 80 µg/ml exhibited similar lethality potential. In another cytotoxicity study, 50 % aqueous ethanol extract of C. bonplandianum showed 100 % mortality at 1 mg/ml concentration [11] .…”

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confidence: 72%

In vitro Screening of Berberis lycium Root Extract on HCT-116 and MCF-7 Cell Lines

Hu¹,

Islam²,

Ameen³

et al. 2020

pharmaceutical-sciences

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Hu et al.: Cytotoxic Screening of Berberis lycium The objective of the current study was to evaluate the antioxidant, lethality and anticancer potential of root extracts of Berberis lycium. Equal weight of powdered roots was used to prepare water, methanol, ethyl acetate and n-hexane extracts. Methanol extract of Berberis lycium showed promising 2,2-diphenyl-1-picrylhydrazyl free radical scavenging potential with an IC 50 of 144.31 µg/ml. In the brine shrimp lethality assay, the ethyl acetate extract produced 53.33 % mortality at 1000 µg/ml and its LD 50 was 907.7 µg/ml. In the sulforhodamine B colorimetric cytotoxicity screening assay, methanol extracts of Berberis lycium caused 45 % inhibition in growth of HCT116, without producing much effect against MCF7 cell line. In addition, the potential mediators that could be involved in mediating these activities include AKT1, TP53, JUN, and TLR4. In conclusion, methanol extract of Berberis lycium exhibited excellent antioxidant, brine shrimp lethality, and cytotoxic activities, indicating the potential of this plant against colorectal and breast cancer.

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“…Antioxidant activity of the extracts was determined on the basis of their scavenging potential of the stable DPPH (1, 1-Diphenyl-2-Picrylhydrazyl) free radical in a quantitative assay. [22][23][24]In short, 2.5 mg extract was mixed with 25 mL of ethanol to prepare 100 μg/mL solution of the extract as stock solution. From the stock solution of the extract, a serial dilution was carried out to obtain a concentration of 1, 5, 10, 50, 100, 500 µg/ml.…”

Section: Antioxidant Testmentioning

confidence: 99%

Comparative Studies on Antioxidant and Thrombolytic Activities of Methanol and Ethylacetate Extracts of Trema Orientalis

Azad¹,

Lasker²,

Rahaman³

et al. 2016

IJIBCS

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The present study was carried out to evaluate in-vitro antioxidant, and thrombolytic activity of MeOH and EtOAc extracts Trema orientalis flowers. Antioxidant activity of the extracts was tested by using DPPH (1, 1diphenyl-2-picrylhydrazyl) free radical scavenging assay method whereas the thrombolytic activity was tested by in-vitro clot lysis assay method. The MeOH extract of T. orientalis showed mild free radical scavenging activity (IC 50 : 131.2µg/ml) compared to ascorbic acid (IC 50 :12.4µg/ml) used as a standard. On the other hand, both MeOH and EtOAc extract produced 21.4 and 21.5% clot lysis respectively compared to streptokinase used as a positive control that caused 51% clot lysis.

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In Vitro Antibacterial Activities and Brine Shrimp Lethality Bioassay of Ethanolic Extract From Moringa Oleifera Lam. Leaves

Cited by 6 publications

References 23 publications

Cytotoxic, in vitro Enzyme Inhibitory Potential and Phytochemical Profiling of Odontonema strictum

Cytotoxic, in vitro Enzyme Inhibitory Potential and Phytochemical Profiling of Odontonema strictum

In vitro Screening of Berberis lycium Root Extract on HCT-116 and MCF-7 Cell Lines

Comparative Studies on Antioxidant and Thrombolytic Activities of Methanol and Ethylacetate Extracts of Trema Orientalis

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