In Vitro Antibacterial Activity of Different Honey Samples Against Clinical Isolates

Abstract

BackgroundThe emergence of multi–drug resistant organisms has created a lot of clinicalproblems. Hence there is a need to find natural alternative treatment to counter the multi–drug resistant organisms. Honey has a well-established usage as wound dressing in ancient and traditional medicine. The aim of this work is to establish a base-line of the antibacterial activity of 32 natural and commercial various honey samples against 8 clinical isolates.MethodsThirty two honey samples (raw and commercial honey) collected from different countries with different floral origin were tested in vitro for antibacterial activity against 8 clinical isolates collected from patients, at private Hospital from Sudan, using disk diffusion technique. The following 6 Epsilometer test (E Test), Amoxicillin, Augamentin, Ceftaxime, Chloramphenicol, Gentamicin and tetracycline were used against 8 clinical isolates for Minimum Inhibitory Concentration.ResultsThe following 8 clinical isolates were identified by conventional bacteriological methods; Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aerouginosa, Proteus.vulgaris, Salmonella typhi, Shigella sonnei and Methicillin Resistant Staphylococcus aureus (MRSA). Honey has been shown to have antibacterial properties against both Gram-positive and Gram-negative bacteria. The tested organisms showed low sensitivity to antibiotics E test.ConclusionMost of the bacterial species studied were uniformly receptive to all the tested honey. In contrast The tested organisms showed low sensitivity to antibiotics with low MIC.

Bioactivity and Chemical Characterization of Sudanese Bee Honey: Crude Acacia and Its Organic Extracts

“…The following 10 standard organisms Gram-positive, Gram-negative, Microbiology Reference Laboratories were obtained, the American Type Culture Collection (ATCC), 12301 Drive, Rockville, MD 20852, USA: Enterobacter aerogenes (E. aerogenes) : ATCC: 13048, Enterococcus faecalis (E.faecalis) : ATCC: 29212, Escherichia coli (E.coli) : ATCC: 25922, Klebsiella pneumoniae (K. pneumoniae) : ATCC: 700603, Pseudomonas aeruginosa (P.aeruginosa) : ATCC: 27853, Serratia marcescens (S.marcescens) : ATCC: 8100, Staphylococcus aureus (S.aureus ) : ATCC: 29213, Staphylococcus epidermidis (S.epidermidis) : ATCC: 12228, Staphylococcus Methicillin Sensitive (MSSA): ATCC: 29213, and Staphylococcus Methicillin -Resistant (MRSA): ATCC: 23591 [ 42 ].…”

“…Control organisms were suspended in a sterile saline to match 0.5 McFarland standard tube, which is commercially available, and provide an optical density of 1.5 × 10 8 colony-forming units (CFU/ml). The well plate technique was used for testing honey antibacterial activity due to its high viscosity [ 42 ]. .…”

“…Four cups (8 mm diameter) were cut using 8-mm sterile cork borer, and the cut disc of agar was removed, and 0.2 ml of each honey sample was carefully added to diffuse. The seeded plates were incubated at 37°C for 18-24 hours [ 42 ]. The diameter of the resultant growth inhibition zone was measured in mm ± standard deviation (SD.…”

Antibacterial Activity of Honey in Combination with Cinnamon

“…Recently, a number of studies have suggested several mechanisms for the antibacterial activity of honey. The high osmotic nature of honey contributes mainly to its antibacterial activity due to the presence of high sugar and low water content (5,12). High osmotic pressure inhibits bacterial growth by drawing water out of bacterial cells.…”

“…Honey has been proven to have important biological properties such as antimicrobial, antioxidant and anti-inflammatory activities (4). Because of that, honey is a popular natural compound used in traditional home remedies and Ayurvedic medicinal system, and it has a long history of being used in the treatment of various infections (5). In previous studies, honey has shown both bactericidal and bacteriostatic properties depending on the concentration of honey used and the type of the bacteria.…”