In Vitro antibacterial activity of rumex nervosus, plantago lanceolata, solanum incanum and lepidium sativum against selected bacterial pathogens of human and animals

Abstract

Medicinal plants are used as a primary means of tackling different ailments especially in developing countries. In the present study, the leaves of Rumex nervosus, Plantago lanceolata, Lepidium sativum and Solanum incanum were collected and extracted by maceration method using 70% methanol. The extracts were concentrated and the yields recorded. Antibacterial effects of the extracts were tested on selected bacteria such as Streptococcus, S. aureus, L. monocytogenes, E. coli, and Salmonella which were obtained from Haramaya University, Veterinary Microbiology Laboratory. Preliminary screening test for the extract was made using agar disc diffusion method and subsequently the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by agar dilution assay based on the zone of bacterial growth inhibition. The finding indicated that Rumex nervosus showed have the highest zone of inhibition (20mm) against the genus Salmonella followed by Plantago lanceolata with the zone of inhibition (16mm) against L. monocytogenes. On the other hand, Lepidium sativum and Solanum incanum did not have any antibacterial activity against all the cultured bacteria. The minimum inhibitory concentration and the minimum bactericidal concentration of Rumex nervosus against S. aureus were 1.56x103μg/ml and that of Plantago lanceolata against L. monocytogenes were 6.25 x103μg/ml and 1.25 x 104μg/ml, respectively. From the findings it can be concluded that Rumex nervosus and Plantago lanceolata have shown to be a better antibacterial activity against selected bacterial pathogens, while leave extracts of other medicinal plants do not have any effect. Fractionated components of the plants might show better effect which needs further investigation using different solvent and standard medium.Keywords: Agar disc diffusion, Antibacterial activity, Rumex nervosus, Plantago lanceolata, Solanum incanum, Lepidium sativum

Antimicrobial activity of Rumex nervosus extract collected from Yemen against local selected isolates pathogens

“…This finding was different with other studies revealed that the least activity was recorded against E. coli. 7,16 In addition, our results was different with a study conducted by Kasimala et al, 17 on methanol extract of Rumex nervosus had also showed no antibacterial activity against E.coli and S. aureus. Where as, our results reported that highly antibacterial activity against E.coli and S. aureus.…”

In vitro phytochemical analysis and antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi

“…Although the results of this study support the findings of Al-Nowihi et al [21] on the high activity of R. nervosus against S. aureus, our results contradict their findings with respect to E. coli, where they demonstrated that R. nervosus had a strong growth inhibitory effect on E. coli among Gram-negative bacteria in both ethanolic and methanolic solvents. However, the obtained result regarding the effect of R. nervosus extracts on E. coli is in agreement with other studies, which showed that the extract of R. nervosus had the lowest anti-E. coli activity [49,50].…”

Antibacterial Activity Against Multidrug-Resistant Clinical Isolates of Nine Plants from Chencha, Southern Ethiopia

“… 49 Also, a previous study done in Ethiopia showed that this plant demonstrated antibacterial activities against Listeria monocytogenes, Streptococcus, S. aureus, Salmonella , and E. coli . 50 In addition, P. lanceolata leaf extract in various solvents also exhibited antibacterial activity against S. pneumoniae , MRSA, S. aureus, S. boydii, E. coli , and K. pneumoniae . 51…”

“…49 Also, a previous study done in Ethiopia showed that this plant demonstrated antibacterial activities against Listeria monocytogenes, Streptococcus, S. aureus, Salmonella, and E. coli. 50 In addition, P. lanceolata leaf extract in various solvents also exhibited antibacterial activity against S. pneumoniae, MRSA, S. aureus, S. boydii, E. coli, and K. pneumoniae. 51 Matricaria recutita was another active plant incorporated in our study; EtOAc and EtOH extracts of this plant revealed promising antimicrobial activity against the test ATCC bacteria, while it showed only a lower activity level against MDR isolates.…”